Volume 8, Issue 3 (Autumn[PERSIAN] 2014)                   mljgoums 2014, 8(3): 103-109 | Back to browse issues page

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Elmi Merian M, Faezi Ghasemi M. Isolation and Characterization of Burkholderia Cepacia Strains from Hospitalized Patients in the Hospitals of West Guilan Province. mljgoums 2014; 8 (3) :103-109
URL: http://mlj.goums.ac.ir/article-1-519-en.html
Isolation and Characterization of Burkholderia Cepacia Strains from Hospitalized Patients in the Hospitals of West Guilan Province
M Elmi Merian , M Faezi Ghasemi 1
1- , faezi_m@yahoo.com
Abstract:   (14039 Views)
Abstract Background and Objective: Burkholderia cepacia complex (BCC) is a plant pathogen that is an important mortality factor in immune-compromised and hospitalized patients. We aimed to Isolate and Characterize the Burkholderia Cepacia Strains from Hospitalized Patients in the Hospitals of West Guilan Province. Material and Methods: This study was conducted on 90 saliva and blood samples obtained from patients with blood infection, pneumonia, asthma, patients connected to the monitoring and ventilator systems, and immune-compromised patients in different sections of hospitals such as the pediatrics, internal section, ICU and CCU. Primary screening was performed by cultivating the samples on Burkholderia cepacia selective agar (BCSA) Sensitivity to antibiotics was tested by Kirby-Bauer and Muller-Hinton Agar (MHA) and the separated isolations were recognized by strengthening the gene rec A. Results: Of 90 isolated samples, only one strain suspected B. cepacia was isolated from 24-year old women with asthma. This strain was resistant to bacitracin, pipracillin and ciprofloxacin antibiotics. Conclusion: The incidence of B. cepacia is rare in western part of Guilan province, which is congruent with the results of overall incidence in Iran.
Keywords: BurkholderiaCepacia, Isolation, Characterization, Nosocomial Infection
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Research Article: Brief Report |
Received: 2014/08/6 | Accepted: 2014/08/6 | Published: 2014/08/6 | ePublished: 2014/08/6
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