This paper should be cited as: Elmi Merian, M. Faezi Ghasemi, M.
Isolation and Characterization of Burkholderia Cepacia Strains from Hospitalized Patients in the Hospitals of West Guilan Province
Elmi Merian, M. (MSc)1, Faezi Ghasemi, M. (PhD)2
1. MSc of Microbiology, Islamic Azad University, Lahijan Branch, Iran
2. Assistant Professor of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Lahijan Branch, Ir
Abstract
Background and Objective:Burkholderia cepacia complex (BCC) is a plant pathogen that is an important mortality factor in immune-compromised and hospitalized patients. We aimed to Isolate and Characterize the Burkholderia Cepacia Strains from Hospitalized Patients in the Hospitals of West Guilan Province.
Material and Methods:This study was conducted on 90 saliva and blood samples obtained from patients with blood infection, pneumonia, asthma, patients connected to the monitoring and ventilator systems, and immune-compromised patients in different sections of hospitals such as the pediatrics, internal section, ICU and CCU. Primary screening was performed by cultivating the samples on Burkholderia cepacia selective agar (BCSA); Sensitivity to antibiotics was tested by Kirby-Bauer and Muller-Hinton Agar (MHA); and the separated isolations were recognized by strengthening the gene rec A.
Results: Of 90 isolated samples, only one strain suspected B. cepacia was isolated from 24-year old women with asthma. This strain was resistant to bacitracin, pipracillin and ciprofloxacin antibiotics.
Conclusion: The incidence of B. cepacia is rare in western part of Guilan province, which is congruent with the results of overall incidence in Iran.
Keywords: BurkholderiaCepacia, Isolation, Characterization, Nosocomial Infection
Corresponding Author: Faezi Ghasemi, M.
Email: faezi_m@yahoo.com
Received: 25 Sep 2013
Revised: 31 Dec 2013
Accepted: 5 Jan 2014