Fri, May 3, 2024
Volume 17, Issue 4 (Jul-Aug 2023)
mljgoums 2023, 17(4): 17-19 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sajjadi S A, Moosavi Z, Niknejad F, Jamshidi A. Effects of kefir on liver function tests and histopathological changes in rats exposed to aflatoxin B1. mljgoums 2023; 17 (4) :17-19
URL: http://mlj.goums.ac.ir/article-1-1559-en.html
URL: http://mlj.goums.ac.ir/article-1-1559-en.html
1- Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2- Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
3- Department of Medical Parasitology and Mycology, Golestan University of Medical Sciences, Gorgan, Iran
4- Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran , ajamshid@ferdowsi.um.ac.ir
2- Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
3- Department of Medical Parasitology and Mycology, Golestan University of Medical Sciences, Gorgan, Iran
4- Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran , ajamshid@ferdowsi.um.ac.ir
Abstract: (808 Views)
Seyed ahmad Sajjadi1 
, Zahra Moosavi2 , Farhad Niknejad3 , Abdollah Jamshidi 4
Background: Aflatoxin B1 (AFB1) is one of the most important mycotoxins that contaminate food worldwide. Long-term consumption of foods contaminated with AFB1 endangers human health. Detoxification of AFB1 from food improves community health. A Specific approach to aflatoxin reduction is the use of probiotics. Kefir drink is a strong probiotic. The purpose of this study was to investigate the protective effect of kefir drink on AFB1-induced hepatic injury in adult male rats
Methods: In this experimental study, 24 adult rats weighing between 150 and 200 g were used. The rats were randomly divided into 4 groups: 1) control, 2) AFB1 (50 μg/kg body weight), 3) kefir drink (10 mL/kg body weight), and 4) AFB1 + kefir drink. Aflatoxin and kefir drink received through oral gavage. At the end of the experiment (8 weeks), blood and liver samples were collected for different assays. Liver function tests and histopathological examinations were performed. Data were analyzed using 1-way analysis of variance (ANOVA) and at a significance level of <0.05.
Results: Aflatoxin B1 significantly increased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (T.Bili), as well as decreased total protein (T.P) content, compared to the control group (P < 0.05). Aflatoxin B1 induced histological changes in the liver. The results obtained from the groups treated with kefir drink with and without AFB1 were not significantly different from the control group. Histopathological changes were not found in groups treated with kefir drink with and without AFB1.
Conclusion: The consumption of kefir drink reduced AFB1-induced disruptions in rats’ livers.
, Zahra Moosavi2 , Farhad Niknejad3 , Abdollah Jamshidi 4Background: Aflatoxin B1 (AFB1) is one of the most important mycotoxins that contaminate food worldwide. Long-term consumption of foods contaminated with AFB1 endangers human health. Detoxification of AFB1 from food improves community health. A Specific approach to aflatoxin reduction is the use of probiotics. Kefir drink is a strong probiotic. The purpose of this study was to investigate the protective effect of kefir drink on AFB1-induced hepatic injury in adult male rats
Methods: In this experimental study, 24 adult rats weighing between 150 and 200 g were used. The rats were randomly divided into 4 groups: 1) control, 2) AFB1 (50 μg/kg body weight), 3) kefir drink (10 mL/kg body weight), and 4) AFB1 + kefir drink. Aflatoxin and kefir drink received through oral gavage. At the end of the experiment (8 weeks), blood and liver samples were collected for different assays. Liver function tests and histopathological examinations were performed. Data were analyzed using 1-way analysis of variance (ANOVA) and at a significance level of <0.05.
Results: Aflatoxin B1 significantly increased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (T.Bili), as well as decreased total protein (T.P) content, compared to the control group (P < 0.05). Aflatoxin B1 induced histological changes in the liver. The results obtained from the groups treated with kefir drink with and without AFB1 were not significantly different from the control group. Histopathological changes were not found in groups treated with kefir drink with and without AFB1.
Conclusion: The consumption of kefir drink reduced AFB1-induced disruptions in rats’ livers.
Research Article: Research Article |
Subject:
Biochemistry
Received: 2022/08/8 | Accepted: 2022/09/8 | Published: 2023/10/2 | ePublished: 2023/10/2
Received: 2022/08/8 | Accepted: 2022/09/8 | Published: 2023/10/2 | ePublished: 2023/10/2
References
1. Gruber-Dorninger C, Jenkins T, Schatzmayr G. Global mycotoxin occurrence in feed: a ten-year survey. Toxins(Basel). 2019;11(7):375. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Kumar P, Mahato DK, Kamle M, Mohanta TK, Kang SG. Aflatoxins: A global concern for food safety, human health and their management. Front Microbiol. 2017;7:2170. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Nazhand A, Durazzo A, Lucarini M, Souto EB, Santini A. Characteristics, occurrence, detection and detoxification of aflatoxins in foods and feeds. Foods. 2020;9(5):644. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Rushing BR, Selim MI. Aflatoxin B1: A review on metabolism, toxicity, occurrence in food, occupational exposure, and detoxification methods. Food Chem Toxicol. 2019;124:81-100. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Sipos P, Peles F, Brassó DL, Béri B, Pusztahelyi T, Pócsi I, et al. Physical and chemical methods for reduction in aflatoxin content of feed and food. Toxins(Basel). 2021;13(3):204. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Afshar P, Shokrzadeh M, Raeisi SN, Ghorbani-HasanSaraei A, Nasiraii LR. Aflatoxins biodetoxification strategies based on probiotic bacteria. Toxicon. 2020;178:50-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Rosa DD, Dias MM, Grześkowiak ŁM, Reis SA, Conceição LL, Maria do Carmo GP. Milk kefir: nutritional, microbiological and health benefits. Nutr Res Rev. 2017;30(1):82-96. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Sharifi M, Moridnia A, Mortazavi D, Salehi M, Bagheri M, Sheikhi A. Kefir: a powerful probiotics with anticancer properties. Med Oncol. 2017;34(11):183. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Zolfaghari H, Khezerlou A, Ehsani A, Khosroushahi AY. Detoxification of Aflatoxin B1 by probiotic Yeasts and Bacteria isolated from dairy products of Iran. Adv Pharm Bull. 2020;10(3):482-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Joint FAO, WHO Food Standards Programme. Codex Alimentarius: Food hygiene basic texts. 3th ed. Rome:Food & Agriculture Org;2004. p.68 [View at Publisher] [Google Scholar]
11. Ahmadi-Noorbakhsh S, Mirabzadeh Ardakani E, Sadighi J, Aldavood SJ, Farajli Abbasi M, Farzad-Mohajeri S, et al. Guideline for the care and use of laboratory animals in Iran. Lab Anim(NY). 2021;50(11):303-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Meki A-RM, Abdel-Ghaffar SK, El-Gibaly I. Aflatoxin B1 induces apoptosis in rat liver: protective effect of melatonin. Neuro Endocrinol Lett. 2001;22(6):417-26. [View at Publisher] [Google Scholar]
13. Thomas L. Clinical laboratory diagnostics. 1ST ed. Frankfurt TH:Books Verlagsgesellschaft;1998. [View at Publisher] [Google Scholar]
14. Abdel-Wahhab MA, El-Nekeety AA, Hathout AS, Salman AS, Abdel-Aziem SH, Sabry BA, et al. Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats. Toxicon. 2020;181:57-68. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Bbosa GS, Kitya D, Odda J, Ogwal-Okeng J. Aflatoxins metabolism, effects on epigenetic mechanisms and their role in carcinogenesis. Health. 2013;5(10A):14-34. [View at Publisher] [DOI] [Google Scholar]
16. Gaspar‐Pintiliescu A, Oancea A, Cotarlet M, Vasile AM, Bahrim GE, Shaposhnikov S, et al. Angiotensin‐converting enzyme inhibition, antioxidant activity and cytotoxicity of bioactive peptides from fermented bovine colostrum. International Journal of Dairy Technology. 2020;73(1):108-16. [View at Publisher] [DOI] [Google Scholar]
17. Rosa DD, Peluzio MdCG, Bueno TP, Cañizares EV, Miranda LS, Dorbignyi BM, et al. Evaluation of the subchronic toxicity of kefir by oral administration in Wistar rats. Nutr Hosp. 2014;29(6):1352-9. [View at Publisher] [Google Scholar]
18. AbdEl-Mogheith S, El-Gendy AO, Sultan S, El-Nesr KA. Exploring the antimicrobial and hepatoprotective effects of kefir; a probiotic fermented milk. J Pure Appl Microbiol. 2017;11(2):759-72. [View at Publisher] [DOI] [Google Scholar]
19. Wade M, Sapcota D, Verma U. Ameliorating aflatoxicosis in commercial broiler chickens by dietary Mycosorb: Heamato-Biochemical studies. Indian Journal of Animal Research. 2018;52(1):46-50. [View at Publisher] [DOI] [Google Scholar]
20. Ozturk B, Guven A. Effect of kefir on gene ezpression profiles of GSTM1 and GSTT1 with antioxidant deffence in the environment of detoxifixation of afflatoxin an in vivo study. International Journal of Basic and Clinical Studies (IJBCS). 2014;3(1):9-24. [Google Scholar]
21. Taheur FB, Mansour C, Chaieb K. Application of Kefir probiotics strains as aflatoxin B1 binder in culture medium, milk and simulated gastrointestinal conditions. Conference on Molecular Biomed Comput & Network Science and Engineering. 2021. [View at Publisher] [Google Scholar]
22. Ansari F, Rezaei K, Khodaiyan F, Rahmani A. Optimisation of aflatoxin B1 reduction in pistachio nuts by kefir grains using statistical experimental methods. Quality Assurance and Safety of Crops & Foods. 2015;8(4):509-18. [View at Publisher] [DOI] [Google Scholar]
23. Eiri A, Niknejad F, Ardebili A. Detoxification of AFB1 by Yeasts Isolates from Kefir and Traditional Kefir-Like Products. Medical Laboratory Journal. 2022;16(4):20-5. [View at Publisher] [DOI] [Google Scholar]
24. Taheur FB, Fedhila K, Chaieb K, Kouidhi B, Bakhrouf A, Abrunhosa L. Adsorption of aflatoxin B1, zearalenone and ochratoxin A by microorganisms isolated from Kefir grains. Int J Food Microbiol. 2017;251:1-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.