Volume 16, Issue 1 (Jan-Feb 2022)                   mljgoums 2022, 16(1): 25-31 | Back to browse issues page

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badeli Z, Haghkhah M, Ghaemi E A. Phytochemical Analysis of Garlic Hydro-alcoholic Extract and Evaluation of its Anti-bacterial Effect on Enterohemorrhagic Escherichia coli in Vitro and ex Vivo. mljgoums. 2022; 16 (1) :25-31
URL: http://mlj.goums.ac.ir/article-1-1378-en.html
1- Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
2- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran , eghaemi@yahoo.com
Abstract:   (419 Views)
Background and objectives: Garlic is a medicinal plant with various health promoting properties including antimicrobial effects. In this study, we investigated in vitro antibacterial effects of garlic hydro-alcoholic extract against enterohemorrhagic Escherichia coli (EHEC).
Methods: Garlic hydro-alcoholic extract was prepared by maceration method.  Phytochemical analysis of the extract was carried out using gas chromatography-mass spectrometry. Minimum inhibitory concentration (MIC) of the extract against EHEC was determined by micro-dilution assay. Cytotoxic effect of the garlic extract on human colon adenocarcinoma cell line (SW480) was assessed using MTT assay. Micro-dilution assay was also used to determine the MIC of the extract against EHEC when co-cultured with SW480 cells.
Results: The amount of organosulfur in garlic extract was 70.91% and the most common organosulfur compounds were trisulfide, di-2-propenyl (34.8%) and diallyl disulfide (14.83%). The MIC of garlic hydro-alcoholic extract on EHEC alone and when co-cultured with SW480 was 12.5 mg/ml. Concentrations of 12.5 mg/ml and 25 mg/ml of the extract significantly reduced the viability of SW480 cells compared to control and concentration of 6.25 mg/ml of garlic extract (p <0.0001).
Conclusion: The garlic hydro-alcoholic extract has inhibitory effects on EHEC in vitro. Therefore, it can be considered a suitable candidate for controlling infections caused by EHEC.
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Research Article: Research Article | Subject: Microbiology
Received: 2021/04/6 | Accepted: 2021/05/22 | Published: 2021/12/29 | ePublished: 2021/12/29

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