Volume 16, Issue 2 (Mar-Apr 2022)                   mljgoums 2022, 16(2): 21-26 | Back to browse issues page

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Jokar M H, Mohamadkhani F, Moradzadeh M, Beygi S, Mohamadkhani A. Nickel Nanoparticles/Recycled Polyethylene Terephthalate Nanofibers Reduce AlgD Expression in Pseudomonas aeruginosa. mljgoums. 2022; 16 (2) :21-26
URL: http://mlj.goums.ac.ir/article-1-1352-en.html
1- Golestan Rheumatology Research Center, Sayad Shirazi Hospital, Golestan University of Medical Sciences, Gorgan, Iran
2- Department of Textile Engineering, Functional Fibrous Structures & Environmental Enhancement (FFSEE), Amirkabir University of Technology, Tehran, Iran
3- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4- Liver and Pancreatobiliary Diseases Research Center, Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran , ashraf_mohamadkhani@yahoo.com
Abstract:   (466 Views)
Background and objectives: Recycled polyethylene terephthalate (RPET) nanofibers have become an important part of human life, with a continuous increase in their production and consumption. Herein, the antibacterial activity of nickel nanoparticles/recycled polyethylene terephthalate nanofibers (NiNP/RPET NF web) was evaluated by analyzing alginate expression in Pseudomonas aeruginosa, as an opportunistic microorganism.
Methods: NiNPs were synthesized and NiNP/RPET NF was produced by adding 25 μg/ml of NiNP to 10% solutions of RPET at a weight ratio of 3%. After exposing P. aeruginosa (PA01) to NiNP/RPET NF, the biofilm-forming capacity was determined and real-time PCR was performed to measure algD expression.
Results: Treatment with 25 μg/ml of NiNP/RPET NF reduced growth of P. aeruginosa on Mueller Hinton agar but did not result in complete inhibition. The biofilm optical density (550 nm) was 0.464 ± 0.021 after treatment with NiNP/RPET NF and 0.082± 0.011 in the absence of NiNP/RPET NF. This indicates the significant reduction of biofilm formation after exposure to NiNP/RPET NF (p=0.01). In addition, a 0.6-fold (p=0.03) reduction in alginate expression was detected by real-time quantitative real-time PCR.
Conclusion: Our results indicate the potential of NiNP/RPET NF for application in nano-based antibacterial medical systems.
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Research Article: Original Paper | Subject: Microbiology
Received: 2020/12/20 | Accepted: 2021/02/7 | Published: 2022/03/7 | ePublished: 2022/03/7

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