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Volume 14, Issue 6 (Nov-Dec 2020)
mljgoums 2020, 14(6): 10-16 |
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zakeri M, Mohebbi A, Askari F S, Yasaghi M. Variations of Histone Acetyltransferase 300 in Patients with Human Papillomavirus Type 6-Associated Anogenital Warts. mljgoums 2020; 14 (6) :10-16
URL: http://mlj.goums.ac.ir/article-1-1326-en.html
URL: http://mlj.goums.ac.ir/article-1-1326-en.html
1- Department of biology, Islamic Azad University, Tehran Medical Branch, Tehran, Iran , taheriforough93@gmail.com@yahoo.com
2- Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranStem Cell Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3- Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
4- Department of Biology, Islamic Azad University of Tehran, Medical Branch, Tehran, Iran
2- Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranStem Cell Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3- Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
4- Department of Biology, Islamic Azad University of Tehran, Medical Branch, Tehran, Iran
Abstract: (2227 Views)
Background and objectives: Pathogenesis of human papillomaviruses (HPVs) is controlled by viral and host factors, among which human histone acetyltransferase p300 (EP300) plays an important role. This study aimed to examine single nucleotide polymorphisms (SNPs) at the EP300 binding site in patients with HPV-associated anogenital wart.
Methods: After DNA extraction, polymerase chain reaction was performed to determine HPV genotypes. Human p300 was amplified to detect SNPs using Sanger sequencing.
Results: Overall, 35.3% of HPV-6-positive patients had Ile997Val substitution at the EP300 binding site. Another SNP containing A to G point mutation leading to Glu983Gly was also detected. In addition, Ile997Val substitution of EP300 was frequently observed in the patients.
Conclusion: Our findings suggest that the EP300 genotype Ile/Val can be involved in HPV-6 pathogenesis. In addition, we introduced a new genotype (Glu983Gly) at the EP300 bromodomain site, which requires further investigation.
Methods: After DNA extraction, polymerase chain reaction was performed to determine HPV genotypes. Human p300 was amplified to detect SNPs using Sanger sequencing.
Results: Overall, 35.3% of HPV-6-positive patients had Ile997Val substitution at the EP300 binding site. Another SNP containing A to G point mutation leading to Glu983Gly was also detected. In addition, Ile997Val substitution of EP300 was frequently observed in the patients.
Conclusion: Our findings suggest that the EP300 genotype Ile/Val can be involved in HPV-6 pathogenesis. In addition, we introduced a new genotype (Glu983Gly) at the EP300 bromodomain site, which requires further investigation.
Keywords: Human Papilloma Virus, Anogenital warts, Histone acetyltransferase p300, Polymorphism, EP300, Single Nucleotide Polymorphism
Research Article: Original Paper |
Subject:
Human Genetics
Received: 2020/05/16 | Accepted: 2020/06/13 | Published: 2020/10/29 | ePublished: 2020/10/29
Received: 2020/05/16 | Accepted: 2020/06/13 | Published: 2020/10/29 | ePublished: 2020/10/29
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