Thu, Dec 5, 2024
Volume 11, Issue 4 (Jul-Aug 2017)
mljgoums 2017, 11(4): 34-38 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Nofouzi K, Rakhshanpoor A, Asadpour R. Antibiotic Susceptibility and Plasmid Profiles of Staphylococcus aureus Strains Isolated from Bovine Subclinical Mastitis in Tabriz, Iran. mljgoums 2017; 11 (4) :34-38
URL: http://mlj.goums.ac.ir/article-1-990-en.html
URL: http://mlj.goums.ac.ir/article-1-990-en.html
1- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
2- Residency student of Small Animal Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3- Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
2- Residency student of Small Animal Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3- Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Abstract: (14099 Views)
ABSTRACT
Background and Objectives: Mastitis caused by Staphylococcus aureus is a widely distributed disease in cattle, goats and sheep. The infection is often subclinical in cattle, leading to reduced milk production and quality, but acute catarrhal or even gangrenous inflammation may also occur. The aim of this study was to investigate resistance of S. aureus isolates from bovine subclinical mastitis to a number of antibiotics.
Methods: Milk samples were collected from 120 cows in different regions of Tabriz, Iran. Milk samples were cultured and bacteriological identification was performed. Antimicrobial susceptibility of the isolates was assessed by determining minimum inhibitory concentration. Plasmid DNA was extracted by an alkaline lysis method.
Results: The highest frequency of resistance was observed against gentamicin (100%) and β- lactam antibiotics including amoxicillin (96%), ampicillin (40%) and penicillin (96%). The isolates mostly contained large plasmids, which might harbor acquired antibiotic resistance.
Conclusion: The results confirm the high frequency of antibiotic resistance among staphylococci isolated from bovine subclinical mastitis.
Keywords: Anti-infective Agents, Cattle, Staphylococcus Aureus, Mastitis, Plasmids.
Background and Objectives: Mastitis caused by Staphylococcus aureus is a widely distributed disease in cattle, goats and sheep. The infection is often subclinical in cattle, leading to reduced milk production and quality, but acute catarrhal or even gangrenous inflammation may also occur. The aim of this study was to investigate resistance of S. aureus isolates from bovine subclinical mastitis to a number of antibiotics.
Methods: Milk samples were collected from 120 cows in different regions of Tabriz, Iran. Milk samples were cultured and bacteriological identification was performed. Antimicrobial susceptibility of the isolates was assessed by determining minimum inhibitory concentration. Plasmid DNA was extracted by an alkaline lysis method.
Results: The highest frequency of resistance was observed against gentamicin (100%) and β- lactam antibiotics including amoxicillin (96%), ampicillin (40%) and penicillin (96%). The isolates mostly contained large plasmids, which might harbor acquired antibiotic resistance.
Conclusion: The results confirm the high frequency of antibiotic resistance among staphylococci isolated from bovine subclinical mastitis.
Keywords: Anti-infective Agents, Cattle, Staphylococcus Aureus, Mastitis, Plasmids.
Research Article: Original Paper |
Received: 2017/08/8 | Accepted: 2017/08/8 | Published: 2017/08/8 | ePublished: 2017/08/8
Received: 2017/08/8 | Accepted: 2017/08/8 | Published: 2017/08/8 | ePublished: 2017/08/8
References
1. Aslantas O, Oztork F, Celebi A, AÇIK L, ERGÜN Y. Characterization of Staphylococcus strains isolated from subclinical mastitis by protein patterns, antibiotic resistance and plasmid profile. Ankara Univ Fak Derg. 2006; 53: 47-51.
2. Kasravi R, Bolourchi M, Farzaneh N, Seifi HA, Barin A. Relationship between in vitro antimicrobial sensitivity of bovine subclinical mastitis isolates and treatment outcome in lactating dairy cows. IJVR. 2010; 11: 249-254.
3. Lyon BR, Skurray R. Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev. 1987; 51(1): 88-134.
4. Turustoglu H, Mudul S, Pehlivanoglu F. Antibiotic susceptibility and β- lactamase prevalence for staphylococci isolated from bovine mastitic milk samples. Acta Veterinaria (Beograd). 2002; 52: 337-344. [DOI:10.2298/AVB0206337T]
5. Anderson DG, McKay LL. Simple and rapid method for isolating large plasmid DNA from lactic Streptococci. Appl. Environ. Microbiol. 1983; 46(3): 549-552.
6. Gooraninejad S, Ghorbanpoor M, Salati AP. Antibiotic susceptibility of Staphylococci isolated from bovine subclinical mastitis. Pak J Bio Sci. 2007; 10: 2781-3. [DOI:10.3923/pjbs.2007.2781.2783]
7. Sakwinska O, Morisset D, Madec JY, Waldvogel A, Moreillon P, Haenni M. Link between genotype and antimicrobial resistance in bovine mastitis-related Staphylococcus aureus strains, determined by compairing Swiss and French isolates from the Rhone valley. Appl Environ Microbiol. 2011; 77(10): 3428-3432. [DOI:10.1128/AEM.02468-10]
8. Bengtsson B, Unnerstad HE, Ekman T, Artursson K, Nilsson-Ost M, Waller KP. Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Vet Microbiol. 2009; 136(1-2): 142-9. doi: 10.1016/j.vetmic.2008.10.024. [DOI:10.1016/j.vetmic.2008.10.024]
9. Szweda P, Schielmann M, Frankowska A, Kot B, Zalewska M. Antibiotic resistance in Staphylococcus aureus strains isolated from cows with mastitis in Easter Poland and analysis of susceptibility of resistant strains to alternative nonantibiotic agentsL Lysostaphin, nisin and polymixin B. J Vet Med Sci. 2014; 76(3): 355-362. [DOI:10.1292/jvms.13-0177]
10. Dowding JE. Mechanism of gentamicin resistance in Staphylococcus aureus. Antimicrob Agents Chemother. 1977; 11(1), 47-50. [DOI:10.1128/AAC.11.1.47]
11. Adwan G. Antibiotic resistance against Staphylococcal isolates recovered from subclinical mastitis in the north of Palestine.The Islamic University Journal. 2006; 14 (1): 1-9.
12. Pavulraj S, Amsaveni S, Kalaiselvi L. Screening of Staphylococcus aureus isolates from mastitis for antibacterial susceptibility pattern and betalactamase production. Tamilnadu J Veterinary & Animal Sciences. 2013; 9(4): 300-307.
13. Preez J .Bovine mastitis therapy and why it fails. Jl S Afr vet Ass. 2000; 71(3): 201-208.
14. Ferreiros CM, Souto MJ, Criado MT. Plasmid pattern analysis in differentiation of Staphylococcus epidermidis isolates. J Hosp Infect. 1992; 22(1): 33-39. [DOI:10.1016/0195-6701(92)90128-9]
15. Lacey RW. Antibiotic resistance plasmids of Staphylococcus aureus and their clinical importance. Bacteriol Rev. 1975; 39(1): 1-32.
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.