Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)                   mljgoums 2015, 9(3): 25-31 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

M Vakili (PhD), N Jomeh pour, E Zarifi, M Baghbanian, A Dehghan, M Sahimi et al . Comparison of Diagnostic Procedures for Platelet Products Contaminated with Strains of Staphylococcus Epidermidis and Klebsiella. mljgoums 2015; 9 (3) :25-31
URL: http://mlj.goums.ac.ir/article-1-734-en.html
1- Faculty of Meicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract:   (12964 Views)

Abstract

Background and Objective: Given that microbial contamination is the third largest cause of mortality caused blood transfusion, the examination of contamination in platelet concentrates is essential in blood transfusion centers. The purpose of this study was to achieve a rapid test for bacterial contamination of platelets concentration.

Material and Methods: This laboratory study was conducted on 14 bags of platelet concentrates prepared from Yazd Blood Transfusion Center. Six platelet bags were infected by Staphylococcus epidermidis; six by Klebsiella with a concentration of 150, 15 and 1.5, and two bags were considered as control. In specific intervals, the bags were sampled aseptically and examined by the methods including culture, gram stain, Glucose and PH measurement.

Result: Due to the presence of dextrose, the initial glucose level of platelet bags was above 300 mg/dl. The mean of  Glucose in contaminated platelet bags was progressively  decreased in  3 days in that it reached  165 mg/dl in the third day ( p = 0.002) . The level of PH had a declining process in that it averagely decreased from PH 7.3 to PH 5.2 (P=0.017(. The results of culturing and smear of the bacteria were different according to the concentrations used in the study.

Conclusion: We can detect the contamination of platelet bags by measuring the level of glucose and PH level in the least amount of time.

Keywords: Blood Platelets; Klebsiella; Staphylococcus Epidermidis.

Research Article: Original Paper |
Received: 2015/10/11 | Accepted: 2015/10/11 | Published: 2015/10/11 | ePublished: 2015/10/11

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.