Mon, Apr 21, 2025
1- Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran , m.ghiasian1@gmail.com
2- Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2- Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Abstract: (385 Views)
Background: Pseudomonas aeruginosa is a common causative agent of hospital-acquired infections and exhibits resistance to many antibiotics, including beta-lactams. One of the mechanisms of resistance to beta-lactams is the MexAB-OprM efflux pump. This study investigates the genetic pattern of resistant P.aeruginosa strains concerning the presence of gene encoding the MexAB-OprM efflux pump.
Methods: One hundred and one clinical isolates of P.aeruginosa were collected from teaching hospitals in Isfahan between 2022 and 2023. Antibiotic susceptibility testing of the isolates was conducted using the disk diffusion method, and strains producing extended-spectrum beta-lactamases (ESBL) were identified by the combined disk method. The gene encoding the MexAB-OprM efflux pump in these strains was investigated by polymerase chain reaction (PCR).
Results: A significant proportion of the 101 P.aeruginosa isolates originated from the emergency department and ICU-2, highlighting the clinical significance of this pathogen in these settings. Meropenem demonstrated a high resistance rate (74%), while gentamicin exhibited lower resistance (33.33%). The prevalence of extended-spectrum beta-lactamases (ESBLs) was 30.3%, and the MexAB-OprM efflux pump gene was identified in 80% of ESBL-producing strains, suggesting a potential role in multidrug resistance.
Methods: One hundred and one clinical isolates of P.aeruginosa were collected from teaching hospitals in Isfahan between 2022 and 2023. Antibiotic susceptibility testing of the isolates was conducted using the disk diffusion method, and strains producing extended-spectrum beta-lactamases (ESBL) were identified by the combined disk method. The gene encoding the MexAB-OprM efflux pump in these strains was investigated by polymerase chain reaction (PCR).
Results: A significant proportion of the 101 P.aeruginosa isolates originated from the emergency department and ICU-2, highlighting the clinical significance of this pathogen in these settings. Meropenem demonstrated a high resistance rate (74%), while gentamicin exhibited lower resistance (33.33%). The prevalence of extended-spectrum beta-lactamases (ESBLs) was 30.3%, and the MexAB-OprM efflux pump gene was identified in 80% of ESBL-producing strains, suggesting a potential role in multidrug resistance.
Research Article: Research Article |
Subject:
Microbiology
Received: 2024/09/7 | Accepted: 2024/12/24
Received: 2024/09/7 | Accepted: 2024/12/24
References
1. Tam VH, Chang KT, Abdelraouf K, Brioso CG, Ameka M, McCaskey LA, et al. Prevalence resistant mechanisms and susceptibility of multidrug-resistant bloodstream isolates of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2010; 54(3): 1160-4. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Thuong M, Arvaniti K, Ruimy R, de la Salmonière P, Scanvic-Hameg A, Lucet JC, et al. Epidemiology of Pseudomonas aeruginosa and risk factors for carriage acquisition in an intensive care unit. J Hosp Infect. 2003; 53(4): 274-82. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Rossi E, La Rosa R, Bartell JA, Marvig RL, Haagensen JAJ, Sommer LM, Molin S, Johansen HK. Pseudomonas aeruginosa adaptation and evolution in patients with cystic fibrosis. Nat Rev Microbiol. 2021; 19(5): 331-342. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Jacoby GA, Munoz-Price LS. The new β-lactamases. New England Journal of Medicine. 2005; 27; 352(4): 380-91. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Sendra E, Fernández-Muñoz A, Zamorano L, Oliver A, Horcajada JP, Juan C, Gómez-Zorrilla S. Impact of multidrug resistance on the virulence and fitness of Pseudomonas aeruginosa: a microbiological and clinical perspective. Infection. 2024; 2:1-34. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Breidenstein EB, de la Fuente-Núñez C, Hancock RE. Pseudomonas aeruginosa: all roads lead to resistance. Trends Microbiol. 2011; 19(8): 419-26. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Pan Y-p, Xu Y-h, Wang Z-x, Fang Y-p, Shen J-l. Overexpression of MexAB-OprM efflux pump in carbapenem-resistant Pseudomonas aeruginosa. Arch Microbiol. 2016; 198(6): 565-71. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Malik T, Naim A. Occurrence of ESBLs in Clinical Isolates of Klebsiella Species and Comparative Analysis of Phenotypic Detection Methods. Anti-Infect Agents. 2020; 18(3): 255-60. [View at Publisher] [DOI] [Google Scholar]
9. Bojary Nasrabadi M, Hajia M. Multidrug-resistant Pseudomonas aeruginosa strains in Tehran reference Burn Hospital Tehran Iran. Afr J Microbiol Res. 2012; 6(7): 1393-1396. [View at Publisher] [DOI] [Google Scholar]
10. Dulanto Chiang A, Dekker JP. Efflux pump-mediated resistance to new beta lactam antibiotics in multidrug-resistant gram-negative bacteria. Communications Medicine. 2024 ;4(1): 170. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Lutz J, Lee J. Prevalence and antimicrobial-resistance of Pseudomonas aeruginosa in swimming pools and hot tubs. Int J Environ Res Public Health. 2011; 8(2): 554-564. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Munde A, Purohit H, Shinde S, Kanwar SS. Prevalence and characterization of metallo-β-lactamase producing Pseudomonas aeruginosa in hospitalized patients. Indian J Med Microbiol. 2011; 29(2): 148-152. [View at Publisher] [Google Scholar]
13. Liu Y, Zhu R, Liu X, Li D, Guo M, Fei B, Ren Y, You X, Li Y. Effect of piperine on the inhibitory potential of MexAB-OprM efflux pump and imipenem resistance in carbapenem-resistant Pseudomonas aeruginosa. Microbial Pathogenesis. 2023; 185: 106397. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Ahmadian L, Haghshenas MR, Mirzaei B, Khalili Y, Goli HR. Role of MexAB-OprM efflux pump in the emergence of multidrug-resistant clinical isolates of Pseudomonas aeruginosa in Mazandaran province of Iran. Molecular Biology Reports. 2023; 50(3): 2603-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Mihani F, Khosravi A. Isolation of Pseudomonas aeruginosa strains producing metallo beta lactamases from infections in burned patients and identification of blaIMP and blaVIM genes by PCR. Iran J Med Microbiol. 2007; 1(1): 23-31. [View at Publisher] [Google Scholar]
16. Malekjamshidi MR, Zandi H, Eftekhar F. Prevalence of extended-spectrum β-lactamase and integron gene carriage in multidrug-resistant Klebsiella species isolated from outpatients in Yazd Iran. Iran J Med Sci. 2020; 45(1): 23-31. [View at Publisher] [PMID] [Google Scholar]
17. Shakibaei M, Mobasheri A, Buschmann K. ß1-integrins co-localize with Na K-ATPase, epithelial sodium channels (ENaC) and voltage activated calcium channels (VACC) in human articular chondrocytes. Matrix Biol. 1999; 18(3): 343-351. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Bharty, SS, Gupta M K, Dhakar, J S, Kumar, V, Bharty, S K. Extended Spectrum Beta Lactamase-Producing Pseudomonas aeruginosa: Phenotypic Identification and Antimicrobial Resistance Pattern. SSR-IIJLS. 2024; 10(3): 5448-5455. [View at Publisher] [DOI] [Google Scholar]
19. Rahmani-Badi A, Abdi-Ali A, Falsafi T, Nikname V. Study of antibiotic resistance by efflux in clinical isolates of Pseudomonas aeruginosa. Pak J Biol Sci. 2007;10(6):924-927. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Najjarpirayeh M, Jafarpour M, Shakibaei M. Prevalence and characteristics of multidrug-resistant Pseudomonas aeruginosa isolated from burn patients in Iran. Burns. 2010;36(4):561-566.
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