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mljgoums 2023, 17(4): 1-4 |
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Matena M, N. Bongo G, Ngbanda H, B. Bakemo E, M. Mukaba Y, K. Munzumba J, et al . Biological evaluation of coagulation problems in COVID-19 patients hospitalized at the centre hospitalier mère-enfant monkole, kinshasa, Democratic Republic of the Congo. mljgoums 2023; 17 (4) :1-4
URL: http://mlj.goums.ac.ir/article-1-1613-en.html
URL: http://mlj.goums.ac.ir/article-1-1613-en.html
Merlin Matena1 
, Gédéon N. Bongo 2, Honoré Ngbanda3 , Eddy B. Bakemo4 , Yves M. Mukaba1 , Jossard K. Munzumba1 , Justin M. Vuvu5 , Christel K. Kande1 , Jacques N. Ngayuna5 , Jonathan I. Kukila6 , Ngbolua Koto-te-Nyiwa7
, Gédéon N. Bongo 2, Honoré Ngbanda3 , Eddy B. Bakemo4 , Yves M. Mukaba1 , Jossard K. Munzumba1 , Justin M. Vuvu5 , Christel K. Kande1 , Jacques N. Ngayuna5 , Jonathan I. Kukila6 , Ngbolua Koto-te-Nyiwa7
1- Service of Laboratory, Department of Medical Biology, Centre Hospitalier Mère et Enfant de Monkole, Kinshasa, Democratic Republic of the Congo/ Department of Medical Biology, Faculty of Health Sciences, Université PanAfricaine du Congo, Kinshasa, Democratic Republic of the Congo
2- Department of Life Sciences, Faculty of Science and Technology, University of Kinshasa, Democratic Republic of the Congo , gedeonbongo@gmail.com
3- Department of Medical Biology, Faculty of Health Sciences, Université PanAfricaine du Congo, Kinshasa, Democratic Republic of the Congo
4- Unit of Biochemistry, Department of Medical Biology, Cliniques Universitaires de Kinshasa, Kinshasa, Democratic Republic of the Congo
5- Service of Laboratory, Department of Medical Biology, Centre Hospitalier Mère et Enfant de Monkole, Kinshasa, Democratic Republic of the Congo
6- Service of Laboratory, Department of Medical Biology, Centre Hospitalier Mère et Enfant de Monkole, Kinshasa, Democratic Republic of the Congo/ Unit of Biochemistry, Department of Medical Biology, Cliniques Universitaires de Kinshasa, Kinshasa, Democratic Republic of the Congo
7- Department of Life Sciences, Faculty of Science and Technology, University of Kinshasa, Democratic Republic of the Congo
2- Department of Life Sciences, Faculty of Science and Technology, University of Kinshasa, Democratic Republic of the Congo , gedeonbongo@gmail.com
3- Department of Medical Biology, Faculty of Health Sciences, Université PanAfricaine du Congo, Kinshasa, Democratic Republic of the Congo
4- Unit of Biochemistry, Department of Medical Biology, Cliniques Universitaires de Kinshasa, Kinshasa, Democratic Republic of the Congo
5- Service of Laboratory, Department of Medical Biology, Centre Hospitalier Mère et Enfant de Monkole, Kinshasa, Democratic Republic of the Congo
6- Service of Laboratory, Department of Medical Biology, Centre Hospitalier Mère et Enfant de Monkole, Kinshasa, Democratic Republic of the Congo/ Unit of Biochemistry, Department of Medical Biology, Cliniques Universitaires de Kinshasa, Kinshasa, Democratic Republic of the Congo
7- Department of Life Sciences, Faculty of Science and Technology, University of Kinshasa, Democratic Republic of the Congo
Abstract: (1009 Views)
Background: COVID-19 is a viral infection caused by SARS-CoV-2, which enters the body via the ACE2 receptor. This study aims to evaluate the coagulation disorders of COVID-19 patients admitted to Centre Hospitalier Mère-Enfant Monkole, Kinshasa.
Methods: This descriptive cross-sectional hospital-based study of patient files was conducted between July 2020 and June 2021 at CHME-Monkole in Kinshasa. The sample size was 130 patients using a random sampling technique after interviewing the respondents. For each respondent, biological and socio-demographic data were collected on a questionnaire. The primary analyses included the determination of PT, APTT, Plasma determination of D-dimers, and platelet count. A descriptive analysis was performed for socio-demographic characteristics, while Pearson correlation was used to determine the associations between socio-demographic characteristics and different biological parameters using SPSS 25.0. For ethical reasons, informed consent from patients was sought, and confidentiality was assured. The authorization was provided by the Ethical Committee of CHME-Monkole (Ethical code: KIN/CHME/04/2020).
Results: The findings showed D-dimer levels higher than 500 µg/L in 87.7% of respondents, prolonged APTT (>40 seconds) in 43.1% of respondents, PT (<70%) in 36.9% of respondents, and thrombocytopenia (platelets <150,000) in 26.2% of respondents. A positive correlation was observed between socio-demographic characteristics and D-dimer levels.
Conclusion: SARS-CoV-2 infection has a significant impact on coagulation. Thus, determining these biomarkers could predict the risk of disease severity or death in patients with COVID-19.
Methods: This descriptive cross-sectional hospital-based study of patient files was conducted between July 2020 and June 2021 at CHME-Monkole in Kinshasa. The sample size was 130 patients using a random sampling technique after interviewing the respondents. For each respondent, biological and socio-demographic data were collected on a questionnaire. The primary analyses included the determination of PT, APTT, Plasma determination of D-dimers, and platelet count. A descriptive analysis was performed for socio-demographic characteristics, while Pearson correlation was used to determine the associations between socio-demographic characteristics and different biological parameters using SPSS 25.0. For ethical reasons, informed consent from patients was sought, and confidentiality was assured. The authorization was provided by the Ethical Committee of CHME-Monkole (Ethical code: KIN/CHME/04/2020).
Results: The findings showed D-dimer levels higher than 500 µg/L in 87.7% of respondents, prolonged APTT (>40 seconds) in 43.1% of respondents, PT (<70%) in 36.9% of respondents, and thrombocytopenia (platelets <150,000) in 26.2% of respondents. A positive correlation was observed between socio-demographic characteristics and D-dimer levels.
Conclusion: SARS-CoV-2 infection has a significant impact on coagulation. Thus, determining these biomarkers could predict the risk of disease severity or death in patients with COVID-19.
Research Article: Research Article |
Subject:
Laboratory Sciences
Received: 2023/01/15 | Accepted: 2023/04/8 | Published: 2023/10/2 | ePublished: 2023/10/2
Received: 2023/01/15 | Accepted: 2023/04/8 | Published: 2023/10/2 | ePublished: 2023/10/2
References
1. Mpiana PT, Ngbolua KT, Tshibangu DST, Kilembe JT, Gbolo BZ, Mwanangombo DT, et al. Identification of potential inhibitors of SARS-CoV-2 main protease from Aloe vera compounds: A molecular docking study. Chem Phys Lett. 2020;754:137751. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Matondo A, Kilembe JT, Mwanangombo DT, Nsimba BM, Gbolo BZ, Bongo GN, et al. Facing COVID-19 via anti-inflammatory mechanism of action: Molecular docking and Pharmacokinetic studies of six anti-inflammatory compounds derived from Passiflora edulis. Journal of Computational Chemistry & Molecular Modeling. 2021;5(1):550-60. [View at Publisher] [DOI] [Google Scholar]
3. Mpiana PT, Ngbolua KN, Tshibangu DST, Kilembe JT, Gbolo BZ, Mwanangombo DT, et al. Aloe vera (L.) Burm. F. as a potential anti-COVID-19 plant: a mini-review of its antiviral activity. European Journal of Medicinal Plants. 2020;31(8):86-93. [View at Publisher] [DOI] [Google Scholar]
4. Ngbolua KN, Mbadiko CM, Matondo A, Bongo GN, Inkoto CL, Gbolo BZ, et al. Review on Ethno-botany, Virucidal Activity, Phytochemistry and Toxicology of Solanum genus: Potential Bio-resources for the Therapeutic Management of Covid-19. European Journal of Nutrition & Food Safety. 2020;12(7):35-48. [View at Publisher] [DOI] [Google Scholar]
5. Walls AC, Park YJ, Tortorici MA, Wall A, Guire AT, Vaesler D. Structure, function, and antigenicity of the SARS-CoV-2 spike glycoprotein. Cell. 2020;183(6):1735. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Zhang H, Penninger JM, Li Y, Zhong N, Slutsky AS. Angiostensin-conerting enzyme 2 (ACE2) as a SARS-CoV-2 receptor: molecular mechanisms and potential therapeutic target. Intensive care Med. 2020;46(4):586-90. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Faucher M, Chevrier A, Gagnon C, Béland A, Corbeil JP. Suivre la propagation de Covid-19 à travers le monde, mise à jour en temps réel. Le Devoir. 2022;0(0):347-361. [View at Publisher] [Google Scholar]
8. El Kettani Z. Une méta-analyse : l'intérêt du dosage des D-Dimères dans la surveillance des patients atteints du Covid-19. Thèse de Master de Biotechnologie Médicale, Faculté de Médecine et de Pharmacie de Rabat, Université Mohammed V de Rabat. 2020;58pp. [Google Scholar]
9. Susen S, Tacquard CA, Godon A, Mansour A, Garrigue D, Nguyen P, et al. Traitement anticoagulant pour la prévention du risque thrombotique chez un patient hospitalisé avec Covid-19 et surveillance de l'hémostase. Propositions du GIHP et du GFHT. 2020:13-20. [View at Publisher] [Google Scholar]
10. Tang N, Li D, Wang X, Sun Z. Abnormal coagulation parameters are associated with poor prognosis in patients with novel coronavirus pneumonia. Journal of Thrombosis and Haemostasie. 2020;18(4):844-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Varga Z, Flammer AJ, Steiger P, Haberecker M, Andermatt R, Zinkernagel AS, et al. Endothelial cell infection and endothelitis in Covid-19. The Lancet. 2020;395(10234):1417-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Iba T, Levy JH, Levi M, Thacil J. Coagulopathy in Covid-19. Journal of Thrombosis and Haemostasis. 2020;18(9):2103-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Godoy LC, Goligher EC, Lawler PR, Slutsky AS, Zarychanski R. Prévoir et gérer la coagulopathie et les manifestations thrombotiques de la Covid-19 sévères. Canadian Medical Association Journal. 2020 ;192(50):E1816-22. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. WHO. Regional office for Africa. Epidémie de la maladie à coronavirus 2019 (Covid-19) en République Démocratique du Congo. Rapport de situation hébdomadaire. 2021. [View at Publisher] [Google Scholar]
15. Mohammed OIA, Abdalla MHA. Determination of Prothrombin Time, Activated Partial Thromboplastin Time and D-Dimer Levels among Malaria Infected Patients in Sinnar State, Sudan. Open Access Library Journal. 2019;6(12):e5975. [View at Publisher] [DOI] [Google Scholar]
16. Yorike D, Kurniawan MR, Syafaat M. Analysis of D-Dimer Level and Prothombin Time (PT) Activated Prothombin Thromboplastin (APTT) on Heparin Administration to COVID-19 Patients. Indonesian Journal of Medical Laboratory Science and Technology. 2022;4(1):91-8. [View at Publisher] [DOI] [Google Scholar]
17. Bashash D, Abolghasemi H, Salari S, Olfatifar M, Eshghi P, Akbari ME. Elevation of D-Dimer, But Not PT and aPTT, Reflects the Progression of COVID-19 Toward an Unfavorable Outcome: A Meta-Analysis. Iranian Journal of Blood & Cancer. 2020;12(2):47-53. [View at Publisher] [Google Scholar]
18. Elkhalifa AME. D-dimer as a predictive and prognostic marker among COVID-19 patients. Saudi Med J. 2022;43(7):723-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Long H, Nie L, Xiang X, Li H, Zhang X, Fu X, Ren H, Liu W, Wang Q and Wu Q. D-Dimer and Prothrombin Time Are the Significant Indicators of Severe COVID-19 and Poor Prognosis. BioMed Res Int. 2020;2020:6159720. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Lumbulumbu KF. Care analyse rapide de genre -Covid-19 : DRC-Nord-Kivu, Sud-Kivu et Kinshasa. Care DRC. 2020 :1-18. [View at Publisher]
21. Tristan V. Plus de 90 % des décès du Covid-19 surviennent chez les plus de 65 ans. Journal le FIGARO. 2021. [View at Publisher]
22. Wu Z, McGoogan JM. Characteristics of and important lessons from the coronavirus disease 2019 (COVID-19) outbreak in China: summary of a report of 72 314 cases from the Chinese Center for Disease Control and Prevention. JAMA. 2020;323(13):1239-42. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Tang N, Bai H, Chen X, Gong J, Li D, Sun Z. Anticoagulant treatment is associated with decreased mortality in severe coronavirus disease 2019 patients with coagulopathy. J Thromb Haemost. 2020;18(5):1094-1099. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Lippi G, Favaloro EJ. D-dimer is associated with severity of coronavirus disease 2019: a pooled analysis. Thromb Haemost. 2020;120(5):876-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
25. Kazemi E, Nejat RS, Ashkan F, Sheibani H. The laboratory findings and different Covid-19 severities: a systematic review and meta-analysis. Ann Clin Microbiol Antimicrob. 2021;20(1):17. [View at Publisher] [DOI] [PMID] [Google Scholar]
26. Jiang SQ, Huang QF, Xie WM, Lv C, Quan XQ. The association between severe Covid-19 and low platelet count: evidence from 31 observational studies involving 7613 participants. Br J Haematol. 2020;190(1):e29-33. [View at Publisher] [DOI] [Google Scholar]
27. Lamouasni K. Biomarqueurs prédictifs de la sévérité de la Covid-19. Thèse de doctorat, Faculté de Médecine et de Pharmacie - Marrakech. Université CADI AYYAD. 2021 : 86 p. [Google Scholar]
28. Lippi G, Plebani M, Henry BM. Thrombocytopenia is associated with severe coronavirus disease 2019 (Covid-19) infections: a meta-analysis. Clin Chim Acta. 2020;506:145-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
29. Teimury A, Khameneh MT, Khaledi EM. Major coagulation disorders and parameters in COVID-19 patients. Eur J Med Res. 2022;27(1):25. [View at Publisher] [DOI] [PMID] [Google Scholar]
30. Guan WJ, Ni ZY, Hu Y, Liang WH, Ou CQ, He JX, et al. Clinical Characteristics of Coronavirus Disease 2019 in China. N Engl J Med. 2020;382(18):1708-20. [View at Publisher] [DOI] [PMID] [Google Scholar]
31. Ranucci M, Ballotta A, Dedda UD, Baryshnikova E, Poli MD, Resta M, et al. The procoagulant pattern of patients with COVID-19 acute respiratory distress syndrome. J Thromb Haemost. 2020;18(7):1747-51. [View at Publisher] [DOI] [PMID] [Google Scholar]
32. Bowles L, Platon S, Yartey N, Dave M, Lee K, Hart DP, MacDonald V, Vert L, Sivapalaratnam S, Pasi KJ and Callum PC. Lupus anticoagulant and abnormal coagulation tests in patients with Covid-19. N Engl J Med. 2020;383(3):288-90. [View at Publisher] [DOI] [PMID] [Google Scholar]
33. Helms J, Tacquard C, Sévérac F, Léonard-Lorant IL, Ohana M, Delabranche X, et al. High risk of thrombosis in patients with severe SARS-CoV-2 infection: a multicenter prospective cohort study. Intensive Care Med. 2020;46(6):1089-98. [View at Publisher] [DOI] [PMID] [Google Scholar]
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