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Volume 16, Issue 5 (Sep-Oct 2022)
mljgoums 2022, 16(5): 26-30 |
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Valizadeh KeshmeshTapeh N, Namroodi S, Taziki S. Impact of Nanochitin on Serum Concentration of Iron and Calcium in Wistar Rats. mljgoums 2022; 16 (5) :26-30
URL: http://mlj.goums.ac.ir/article-1-1448-en.html
URL: http://mlj.goums.ac.ir/article-1-1448-en.html
1- (MSc) Department of environmental Sciences, Faculty of fisheries and environmental Sciences
2- (PhD) Department of environmental Sciences, Faculty of fisheries and environmental Sciences , namroodi@gau.ac.ir
3- ischemic disorders research center ٫ golestan university of medical sciences ,gorgan ,iran
2- (PhD) Department of environmental Sciences, Faculty of fisheries and environmental Sciences , namroodi@gau.ac.ir
3- ischemic disorders research center ٫ golestan university of medical sciences ,gorgan ,iran
Abstract: (2202 Views)
Background and objectives: Considering the increasing use of nanochitin for the removal of heavy metals from aqueous solutions, examining the biological effects of this substance on the level of essential metals for humans and animals is crucial. Therefore, this study investigated impact of oral administration of nanochitin on serum levels of iron (Fe) and calcium (Ca) in Wistar rats.
Methods: Twenty male Wistar rats were randomly divided into four treatment groups and one control group. Two groups were fed with nanochitin at doses of 1.6 and 2.6 μg/g for 6 weeks, and the other two groups received the mentioned doses for 10 weeks. Serum concentrations of Fe and Ca were measured using atomic absorption spectroscopy.
Results: Oral administration of 2.6 μg/g nanochitin for 10 weeks caused a significant decrease in serum Ca and Fe concentrations (p<0.05). Oral administration of 1.6 and 2.6 μg/g nanochitin for 6 weeks caused a non-significant reduction in serum Fe and Ca concentrations (p>0.05). However, nanochitin consumption for 10 weeks resulted in a significant decrease in serum Fe concentration but not Ca.
Conclusion: The limited reduction of serum Fe and Ca concentrations after oral consumption of nanochitin at a low dose and for a limited duration indicates that the controlled use of nanochitin could be safe for animals. However, complementary studies are needed to determine the exact effects of nanochitin on the animals’ bodies. On the other hand, it is recommended to use Fe and Ca supplements after consuming high doses of nanochitin for longer periods.
Methods: Twenty male Wistar rats were randomly divided into four treatment groups and one control group. Two groups were fed with nanochitin at doses of 1.6 and 2.6 μg/g for 6 weeks, and the other two groups received the mentioned doses for 10 weeks. Serum concentrations of Fe and Ca were measured using atomic absorption spectroscopy.
Results: Oral administration of 2.6 μg/g nanochitin for 10 weeks caused a significant decrease in serum Ca and Fe concentrations (p<0.05). Oral administration of 1.6 and 2.6 μg/g nanochitin for 6 weeks caused a non-significant reduction in serum Fe and Ca concentrations (p>0.05). However, nanochitin consumption for 10 weeks resulted in a significant decrease in serum Fe concentration but not Ca.
Conclusion: The limited reduction of serum Fe and Ca concentrations after oral consumption of nanochitin at a low dose and for a limited duration indicates that the controlled use of nanochitin could be safe for animals. However, complementary studies are needed to determine the exact effects of nanochitin on the animals’ bodies. On the other hand, it is recommended to use Fe and Ca supplements after consuming high doses of nanochitin for longer periods.
Research Article: Original Paper |
Subject:
Laboratory Sciences
Received: 2021/10/18 | Accepted: 2021/12/8 | Published: 2022/09/6 | ePublished: 2022/09/6
Received: 2021/10/18 | Accepted: 2021/12/8 | Published: 2022/09/6 | ePublished: 2022/09/6
References
1. Pinto PX, Al-Abed SR, Reisman DJ. Biosorption of heavy metals from mining influenced water onto chitin products. Chemical Engineering Journal. 2011 February؛ 166(3): 1002-1009. [View at Publisher] [DOI:10.1016/j.cej.2010.11.091.] [Google Scholar]
2. Hsin YH, Chen CF, Huang S, Shih TS, Lai PS, Chueh PJ. The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells. Toxicol Lett. 2008;179(3):130-9. [View at Publisher] [DOI:10.1016/j.toxlet.2008.04.015.] [PubMed] [Google Scholar]
3. Musgrave C.B, Perry J.K, Merkle R.C, & Goddard W.A. Theoretical studies of a hydrogen abstraction tool for nanotechnology. Nanotechnology. 1991; 2(4): 187. [DOI:10.1088/0957-4484/2/4/004] [Google Scholar]
4. Tan TS, Chin HY, Tsai ML, Liu CL. Structural alterations, pore generation, and deacetylation of α- and β-chitin submitted to steam explosion. Carbohydr Polym. 2015;122: 321-8. [View at Publisher] [DOI:10.1016/j.carbpol.2015.01.016.] [PubMed] [Google Scholar]
5. Ghourbanpour J, Sabzi M, Shafagh N. Effective dye adsorption behavior of poly(vinyl alcohol)/chitin nanofiber/Fe(III) complex. Int J Biol Macromol. 2019; 137: 296-306.
https://doi.org/10.1016/j.ijbiomac.2019.06.213 [View at Publisher] [DOI:10.1016/j.ijbiomac.2019.06.213.] [PubMed] [Google Scholar]
6. Latunde-Dada G.O, McKie A.T, & Simpson R. J. Animal models with enhanced erythropoiesis and iron absorption. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2006 April؛ 1762(4): 414-423.
https://doi.org/10.1016/j.bbadis.2005.12.007 [View at Publisher] [DOI:10.1016/j.bbadis.2005.12.007.] [PubMed] [Google Scholar]
7. Schumann K. Safety aspects of iron in food. Annals of Nutrition and Metabolism. 2001; 45: 91–101. [View at Publisher] [DOI:10.1159/000046713.] [Google Scholar]
8. Weaver CM, Heaney RP. Calcium, Modern Nutrition in Health and Disease. 10. Advances in Nutrition. Lippincott Williams & Wilkins. 2006; 2(30): 290-292. [View at Publisher]
9. Institute of Medicine (US) Committee to Review Dietary Reference Intakes for Vitamin D and Calcium. Dietary Reference Intakes for Calcium and Vitamin D. Ross AC, Taylor CL, Yaktine AL, Del Valle HB, editors. Washington (DC): National Academies Press (US); 2011. PMID: 21796828. [View at Publisher] [PubMed] [Google Scholar]
10. Buerge T. and Weiss T. The laboratory Mice. Copy right Elsevier ISBEN.0-1233-6425-6.2004.
11. Al-Attar AM. Antioxidant effect of vitamin E treatment on some heavy metals-induced renal and testicular injuries in male mice. Saudi J Biol Sci. 2011; 18(1): 63-72. [View at Publisher] [DOI:10.1016/j.sjbs.2010.10.004] [PubMed] [Google Scholar]
12. Khazaeipour A, Nimrodi S, and Taziki Sh. The effect of nanokitin on the tissue uptake of lead acetate in the liver of rats. Scientific Journal of Gorgan University of Medical Sciences. 2019; 22 (74): 39-34. [Persian] [View at Publisher]
13. Zarei K, Najafpour Gh, Sharifzadeh M. Synthesis and Application of Nano-chitosan in Removal of Heavy Metals. Thesis in Chemical. Faculty of Chemical Engineering. Babol Noshirvani University of Technology (BUT). 2012. [Persian]
14. CCAC- Canadian Council On Animal Care . The care and use of farm animals in research, teaching and testing. Ottawa. 2009;12-5. [View at Publisher]
15. Yoo S, Chio KS, Ryu MH. A Study on the Effect of Chitin, Chitosan and Dithiocarbamate Chitosan on the Nickel Toxicity in Rat liver. Korean journal of environmental health. 2008 July؛ 34(4):285-91. 2008;,,
https://doi.org/10.5668/JEHS.2008.34.4.285 [View at Publisher] [DOI:10.5668/JEHS.2008.34.4.285.] [Google Scholar]
16. Allen LH. Calcium bioavailability and absorption: a review. Am J Clin Nutr. 1982; 35(4): 783-808. [DOI:10.1093/ajcn/35.4.783.] [PubMed] [Google Scholar]
17. Guthrie H.A. Basics of nutrition. Translated by Dr. Minoo Forouzani. Entesharat sherkate sahami Chehr Tehran. [Persian]
18. Wang B, Feng W, Wang M, Wang T, Gu Y, Zhu M, et al. Acute toxicological impact of nano-and submi- cro-scaled zinc oxide powder on healthy adult mice. Journal of Nanoparticle Research. 2008;10: 263-276. [View at Publisher] [DOI:10.1007/s11051-007-9245-3]
19. Ounjaijean S, Thephinlap C, Khansuwan U, Phisalapong C, Fucharoen S, Porter JB, Srichairatanakool S. Effect of green tea on iron status and oxidative stress in iron-loaded rats. Med Chem. 2008; 4(4): 365-70. [DOI:10.2174/157340608784872316] [PubMed] [Google Scholar]
20. Mehri Pirayvatlu A, Ali Panah Mogadam R, Mazani M, Manafi f, Malekzadeh V, Nemati A, Nagizadeh Bagi A. The effect of andrographolide extract on blood glucose and lipid profile in rats with secondary iron overload. Journal of Ardabil University of Medical Sciences. 2016; 16(4): 399-408. [View at Publisher]
21. Turgut G, Kaptanoğlu B, Turgut S, Enli Y, Genç O. Effects of chronic aluminum administration on blood and liver iron-related parameters in mice. Yonsei Med J. 2004; 45(1): 135-9. [DOI:10.3349/ymj.2004.45.1.135] [PubMed] [Google Scholar]
22. Moshtagi M, Moshtagi A, Mahdavi J, Pourmoghadas H. The effect of fluoride on the concentration of parameters related to iron metabolism in rats. Proceedings of the 3rd Congress of Rare Elements of Iran. Kashan University of Medical Sciences. 2012; 16(7): 715-716. [Persian] [View at Publisher]
23. Wang C, Lu J, Zhou L, Li J, Xu J, Li W. Effects of Long-Term Exposure to Zinc Oxide Nanoparticles on Development, Zinc Metabolism and Biodistribution of Minerals (Zn, Fe, Cu, Mn) in Mice. PLoS ONE. 2016; 11(10): e0164434. [View at Publisher] [DOI:10.1371/journal.pone.0164434] [PubMed]
24. Tavaria FK, Jorge MP, Ruiz LT, Ana Lúcia TG, Pintado ME, Carvalho JE. Anti-proliferative, anti- inflammatory, anti-ulcerogenic and wound healing properties of chitosan. Curr Bioact Comp. 2016; 12(2): 122-114. [DOI:10.2174/1573407212666160330204522] [Google Scholar]
25. Younes H, Coudray C, Bellanger J, Demigné C, Rayssiguier Y, Rémésy C. Effects of two fermentable carbohydrates (inulin and resistant starch) and their combination on calcium and magnesium balance in rats. Br J Nutr. 2001; 86(4): 479-85. [View at Publisher] [DOI:10.1079/BJN2001430.] [PubMed]
26. Shokravi S, Mohamad shirazi M, Abadi A, Komeyli fonud R, Kimiyagar R. The effect of phytase supplementation on the status of zinc, iron and calcium in rats fed a diet containing Iranian perfitate bread (Sangak). Iranian Journal of Endocrinology and Metabolism. Shahid Beheshti University of Medical Sciences and Health Services. 2012; 13(5) :514-523. [Persian] [View at Publisher]
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