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mljgoums 2023, 17(6): 16-18 |
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fatemi M, Ghandehari F, Salehi D, Torabian P. Comparing the cytotoxic effects of iron oxide nanoparticles synthesized using the cytoplasmic extract of Lactobacillus casei and chemical synthesis. mljgoums 2023; 17 (6) :16-18
URL: http://mlj.goums.ac.ir/article-1-1410-en.html
URL: http://mlj.goums.ac.ir/article-1-1410-en.html
1- Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2- Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran ,fe_gh_2010@yahoo.com
3- Department of Chemical Engineering, Isfahan University of Technology, Isfahan, Iran
4- Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2- Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran ,
3- Department of Chemical Engineering, Isfahan University of Technology, Isfahan, Iran
4- Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Abstract: (553 Views)
Background: Discovering new cytotoxic compounds has received significant attention due to the rise in drug resistance and the adverse effects associated with chemotherapy drugs. In this study, the cytoplasmic extract of Lactobacillus casei was used to produce iron oxide nanoparticles (Fe2o3 NPs), and the cytotoxic effects of NPs were investigated on MCF-7 and human embryonic kidney 293 (HEK-293) cells.
Methods: The cytoplasmic extract of L. casei was mixed with 103M iron sulfate solution and incubated for 3 weeks at 37 °C and 5% CO2. The coprecipitation method was used to synthesize chemical Fe2o3 NPs. The synthesis of NPs was studied by electron microscopy and X-ray diffraction (XRD) analysis, and the cytotoxic effects were evaluated with dilutions (10, 100, and 1000 µg/mL) on MCF-7 and HEK cells.
Results: X-ray diffraction analysis and scanning electron microscopy presented the mean of NPs synthesized by the green method to be about 15 nm and their shape to be spherical, as well as the average of chemically synthesized NPs to be about 20 nm with cubic structure. Chemical and green synthesized NPs only at a concentration of 1000 µg/mL were able to significantly reduce the survival rate of normal HEK-293 cells; chemically synthesized NPs decreased MCF-7 cell survival only at 1000 µg/mL and green synthesis at 100 µg/mL and 1000 µg/mL.
Conclusion: Generating Fe2o3 NPs is biologically safe using the green synthesis method and the cytoplasmic extract of L. casei, which may be a suitable candidate for the treatment of cancer cells.
Methods: The cytoplasmic extract of L. casei was mixed with 103M iron sulfate solution and incubated for 3 weeks at 37 °C and 5% CO2. The coprecipitation method was used to synthesize chemical Fe2o3 NPs. The synthesis of NPs was studied by electron microscopy and X-ray diffraction (XRD) analysis, and the cytotoxic effects were evaluated with dilutions (10, 100, and 1000 µg/mL) on MCF-7 and HEK cells.
Results: X-ray diffraction analysis and scanning electron microscopy presented the mean of NPs synthesized by the green method to be about 15 nm and their shape to be spherical, as well as the average of chemically synthesized NPs to be about 20 nm with cubic structure. Chemical and green synthesized NPs only at a concentration of 1000 µg/mL were able to significantly reduce the survival rate of normal HEK-293 cells; chemically synthesized NPs decreased MCF-7 cell survival only at 1000 µg/mL and green synthesis at 100 µg/mL and 1000 µg/mL.
Conclusion: Generating Fe2o3 NPs is biologically safe using the green synthesis method and the cytoplasmic extract of L. casei, which may be a suitable candidate for the treatment of cancer cells.
Research Article: Research Article |
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Received: 2021/07/25 | Accepted: 2021/10/13 | Published: 2024/02/26 | ePublished: 2024/02/26
Received: 2021/07/25 | Accepted: 2021/10/13 | Published: 2024/02/26 | ePublished: 2024/02/26
References
1. Zhang D, Ma X, Gu Y, Huang H, Zhang G. Green Synthesis of Metallic Nanoparticles and Their Potential Applications to Treat Cancer. Front Chem.2020;8:1-18. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Koul B, Poonia AK, Yadav D, Jin JO. Microbe-Mediated Biosynthesis of Nanoparticles: Applications and Future Prospects. Biomolecules. 2021;11(6):886. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Vijayanandan AS, Balakrishnan RM. Biosynthesis of cobalt oxide nanoparticles using endophytic fungus Aspergillus nidulans. J Environ Manage. 218;218:442-50. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Kim DY, Saratale RG, Shinde S, Syed A, Ameen F, Ghodake G. Green synthesis of silver nanoparticles using Laminaria japonica extract: Characterization and seedling growth assessment. J Clean Prod. 2018;172:2910-18. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Omidi B, Hashemi SJ, Bayat M, Larijani K. Biosynthesis of Silver Nanoparticles by Lactobacillus fermentum. Bull Environ Pharmacol Life Sci. 2014;3(12):186‐92. [View at Publisher] [Google Scholar]
6. Torabian P, Ghandehari F, Fatemi M. Evaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei. J Babol Univ Med Sci. 2019;21(1):237-41. [View at Publisher] [DOI] [Google Scholar]
7. Kandpal ND, Sah N, Loshali R, Joshi R, Prasad J. Co-precipitation method of synthesis and characterization of iron oxide nanoparticles. Journal of Scientific and Industrial Research. 2014;73(02):87-90. [View at Publisher] [Google Scholar]
8. Ghandehari F, Rezaee M, Fatemi M. Study on the antimicrobial effects of iron oxide nanoparticles synthesized by cytoplasmic extract of lactobacillus fermentum. New Cellular and Molecular Biotechnology Journal. 2021;9(36):89-96. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Rezaee M, Ghandehari F, Fatemi M, Fani M, Salehi D. Study on the Cytotoxic Effects of Iron Oxide Nanoparticles Synthesized by Cytoplasmic Extract of Lactobacillus Fermentum. Journal of Kerman University of Medical Sciences. 2022;29(1):31-8. [View at Publisher] [DOI] [Google Scholar]
10. Aghebati‐Maleki A, Dolati S, Ahmadi M, Baghbanzhadeh A, Asadi M, Fotouhi A, et al. Nanoparticles and cancer therapy: Perspectives for application of nanoparticles in the treatment of cancers. J Cell Physiol. 2020;235(3):1962-72. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Rana A, Yadav K, Jagadevan S. A comprehensive review on green synthesis of nature-inspired metal nanoparticles: Mechanism, application and toxicity. Journal of Cleaner Production. 2020;272(5):122880. [View at Publisher] [DOI] [Google Scholar]
12. Mandava K, Kadimcharla K, Keesara NR, Fatima SN, Bommena P, Batchu UR. Green Synthesis of Stable Copper Nanoparticles and Synergistic Activity with Antibiotics. Indian Journal of Pharmaceutical Sciences. 2017;79(5):695-700. [View at Publisher] [DOI] [Google Scholar]
13. Qamar H, Rehman S, Chauhan DK, Tiwari AK, Upmanyu V. Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from Momordica charantia. Int J Nanomedicine. 2020;15:2541-53. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Sayadi MH, Salmani N, Heidari A, Rezaei MR. Bio-synthesis of palladium nanoparticle using Spirulina platensis alga extract and its application as adsorbent. Surfaces and Interfaces. 2018;10:136-43. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Mirjani R, Faramarzi MA, Sharifzadeh M, Setayesh N, Khoshayand MR, Shahverdi AR. Biosynthesis of tellurium nanoparticles by Lactobacillus plantarum and the effect of nanoparticle‐enriched probiotics on the lipid profiles of mice. IET Nanobiotechnol. 2015;9(5):300-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Waghmare SR, Mulla MN, Marathe SR, Sonawane KD. Ecofriendly production of silver nanoparticles using Candida utilis and its mechanistic action against pathogenic microorganisms. 3 Biotech. 2015;5(1):33-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Roychoudhury P, Gopal PK, Paul S, Pal R. Cyanobacteria assisted biosynthesis of silver nanoparticles-a potential antileukemic agent. Journal of Applied Phycology. 2016;28(6):3387-94. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Sayed Ahmad M, Mohamed Yasser M, Nageh Sholkamy E, Mohamed Ali A, Mohamed Mehanni M. Anticancer activity of biostabilized selenium nanorods synthesized by Streptomyces bikiniensis strain Ess_amA-1. Int J Nanomedicine. 2015;10:3389-401. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Ramya S, Shanmugasundaram T, Balagurunathan R. Biomedical potential of actinobacterially synthesized selenium nanoparticles with special reference to anti-biofilm, anti-oxidant, wound healing, cytotoxic and anti-viral activities. J Trace Elem Med Biol. 2015;32:30-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Abd-Elnaby HM, Abo-Elala GM, Abdel-Raouf UM, Hamed MM. Antibacterial and anticancer activity of extracellular synthesized silver nanoparticles from marine Streptomyces rochei MHM13. The Egyptian Journal of Aquatic Research. 2016;42(3):301-12. [View at Publisher] [DOI] [Google Scholar]
21. Fariq A, Khan T, Yasmin A. Microbial synthesis of nanoparticles and their potential applications in biomedicine. Journal of Applied Biomedicine. 2017;15(4):241-8. [View at Publisher] [DOI] [Google Scholar]
22. Salem SS, Fouda A. Green Synthesis of Metallic Nanoparticles and Their Prospective Biotechnological Applications: an Overview. Biol Trace Elem Res. 2021;199(1):344-70. [View at Publisher] [DOI] [PMID] [Google Scholar]
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.