Volume 17, Issue 6 (Nov-Dec 2023)                   mljgoums 2023, 17(6): 13-15 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Naghizadeh M S, Naseri M, Anani Sarab G, Derakhshani A, Fereidouni M. The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018. mljgoums 2023; 17 (6) :13-15
URL: http://mlj.goums.ac.ir/article-1-1382-en.html
1- . Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
2- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
3- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran , msnaghizadeh92@bums,ac,ir
Abstract:   (506 Views)
Background: Atopic dermatitis (AD) is a common allergic disorder. Detection of responsible pathogenic allergens in AD patients by reliable methods has a fundamental role in the prevention, management, and treatment of AD. This study was conducted to determine the most common allergens by the skin prick test (SPT) and immunoblotting among AD patients referring to an allergy clinic in Birjand City, Iran.
Methods: The presence of AD was confirmed by an expert allergist. Serum levels of total and specific immunoglobulin E (sIgE) against 30 food and inhalant allergens were evaluated by a commercial immunoblotting kit (AlleisaScreen).
Results: The skin prick test was performed by a battery of 17 allergens. In total, 34 AD patients (mean age, 28.76 ± 17.36 years; range, 1-60 years; F/M ratio: 0.88) were enrolled in this study. The sensitization rates to at least 1 fungus, pollen, food, or indoor allergen by the immunoblotting method were 32.35%, 61.76%, 52.94%, and 47.05%, respectively. The most prevalent allergens were ragweed (52.94), Olive tree (41.16), Eucalyptus (35.29), date palm (35.29), and grass mix (32.28).
Conclusion: The study found that 85.29% of the studied population were sensitized to at least 1 allergen. Pollens and date palms were the most common allergens among AD patients, but the pattern of sensitization in SPT and immunoblotting was not exactly similar. Detection of allergens to which patients are sensitized and avoidance can help in the management of the disease and its symptoms.

Full-Text [PDF 403 kb]   (163 Downloads) |   |   Full-Text (HTML)  (91 Views)  
Research Article: Research Article | Subject: Immunology
Received: 2021/04/16 | Accepted: 2023/05/22 | Published: 2024/02/26 | ePublished: 2024/02/26

1. Ziyaei T, Berenji F, Jabbari-Azad F, Fata A, Jarahi L, Fereidouni M. House dust mite prevalence in the house of patients with atopic dermatitis in Mashhad, Iran. J Arthropod Borne Dis. 2017;11(2):309-14. [View at Publisher] [PMID] [Google Scholar]
2. Salehi T, Pourpak Z, Karkon S, Shoormasti RS, Sabzevari SK, Movahedi M, et al. The Study of Egg Allergy in Children With Atopic Dermatitis. World Allergy Organ J. 2009;2(7):123-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Röckmann H, van Geel MJ, Knulst AC, Huiskes J, Bruijnzeel-Koomen CA, de Bruin-Weller MS. Food allergen sensitization pattern in adults in relation to severity of atopic dermatitis. Clin Transl Allergy. 2014;4(1):9. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Kupryś-Lipińska I, Elgalal A, Kuna P. The epidemiology of atopic dermatitis in the general population of the Lodz province's citizens. Pneumonol Alergol Pol. 2009;77(2):145-51. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Benedé S, Blázquez AB, Chiang D, Tordesillas L, Berin MC. The rise of food allergy: Environmental factors and emerging treatments. EBioMedicine. 2016;7:27-34. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Heinzerling LM, Burbach GJ, Edenharter G, Bachert C, Bindslev-Jensen C, Bonini S, et al. GA(2)LEN skin test study I: GA(2)LEN harmonization of skin prick testing: novel sensitization patterns for inhalant allergens in Europe. Allergy. 2009;64(10):1498-506. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Davari MH, Fereidouni M, Rahimi M. Rate of allergic disorders and skin prick sensitivity to common allergens among patients suffering from pterygium in Birjand, Iran. Guoji Yanke Zazhi. 2018;18(9):1567-71. [View at Publisher] [Google Scholar]
8. Fereidouni M, Bakhshaei M, Varasteh A. Aeroallergen sensitivity of Iranian patients with allergic rhinitis. World Allergy Organization Journal. 2007. [View at Publisher] [DOI] [Google Scholar]
9. Moghtaderi M, Hejrati Z, Kolahi N, Heidari B. Sensitization to aeroallergens in patients with allergic rhinitis, asthma, and atopic dermatitis in Shiraz, Southwestern Iran. Indian Journal of Allergy, Asthma and Immunology. 2015;29(2):79-83. [View at Publisher] [Google Scholar] [View at Publisher] [DOI] [Google Scholar]
10. Bonyadi M, Ezzati F. Common Allergens in Patients with Atopic Dermatitis. Medical Laboratory Journal. 2014;8(2):67-75. [View at Publisher] [Google Scholar]
11. Sybilski AJ, Zalewska M, Furmanczyk K, Lipiec A, Krzych-Falta E, Samolinski B. The prevalence of sensitization to inhalant allergens in children with atopic dermatitis. Allergy Asthma proc. 2015;36(5):e81-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Moghtaderi M, Farjadian S, Kashef S, Alyasin S, Afrasiabi M, Orooj M. Specific IgE to common food allergens in children with atopic dermatitis. Iran J Immunol. 2012;9(1):32-8. [View at Publisher] [PMID] [Google Scholar]
13. Ghaderi R, Rashavi Z. Prevalence of common allergens among patients with atopic dermatitis in Eastern Iran. MOJ Immunol. 2018;6(3):74-80. [View at Publisher] [Google Scholar] [DOI] [View at Publisher] [DOI] [Google Scholar]
14. Bonyadi M, Hassanzadeh D, Seyfizadeh N, Borzoueisileh S. Assessment of allergen-specific IgE by immunoblotting method in atopic dermatitis. Eur Ann Allergy Clin Immunol. 2017;49(5):213-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Alqahtani JM. Asthma and other allergic diseases among Saudi schoolchildren in Najran: the need for a comprehensive intervention program. Ann Saudi Med. 2016;36(6):379-85. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Al-Tamemi S, Shafiq-Ur-Rehman Naseem MT, Alrahman MA-K, Alshekaili J. Food allergen sensitisation patterns in Omani patients with allergic manifestations. Sultan Qaboos Univ Med J. 2018;18(4):e483-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Worm M, Forschner K, Lee H-H, Roehr CC, Edenharter G, Niggemann B, et al. Frequency of atopic dermatitis and relevance of food allergy in adults in Germany. Acta Derm Venereol. 2006;86(2):119-22. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Owczarek W, Paluchowska E, Targowski T. The relation of atopy patch tests with immunoglobulin E. Atopy patch tests with aeroallergens results dependence with the concentration of specific class E antibodies assay level assessment. Pol Merkur Lekarski. 2009;26(151):35-9. [View at Publisher] [PMID] [Google Scholar]
19. De Benedictis FM, Franceschini F, Hill D, Naspitz C, Simons F, Wahn U, et al. The allergic sensitization in infants with atopic eczema from different countries. Allergy. 2009;64(2):295-303. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Kwaasi AA, Harfi HA, Parhar RS, Al‐Sedairy ST, Collison KS, Panzani RC, et al. Allergy to date fruits: characterization of antigens and allergens of fruits of the date palm (Phoenix dactylifera L.). Allergy. 1999;54(12):1270-7. [View at Publisher] [Google Scholar] [DOI] [PMID] [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Asero R, Monsalve R, Barber D. Profilin sensitization detected in the office by skin prick test: a study of prevalence and clinical relevance of profilin as a plant food allergen. Clin Exp Allergy. 2008;38(6):1033-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Kwaasi A, Harfi H, Parhar R, Saleh S, Collison K, Panzani R, et al. Cross‐reactivities between date palm (Phoenix dactylifera L.) polypeptides and foods implicated in the oral allergy syndrome. Allergy. 2002;57(6):508-18. [View at Publisher] [DOI:] [PMID] [Google Scholar]
23. Shoormasti RS, Fazlollahi MR, Kazemnejad A, Movahedi M, Tayebi B, Yazdanyar Z, et al. Accuracy of immunoblotting assay for detection of specific IgE compared with ImmunoCAP in allergic patients. Electron physician. 2018;10(2):6327-32. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Gendo K, Larson EB. Evidence-based diagnostic strategies for evaluating suspected allergic rhinitis. Ann Intern Med. 2004;140(4):278-89. [View at Publisher] [DOI] [PMID] [Google Scholar]
25. De Vos G, Nazari R, Ferastraoaru D, Parikh P, Geliebter R, Pichardo Y, et al. Discordance between aeroallergen specific serum IgE and skin testing in children younger than 4 years. Ann Allergy Asthma Immunol. 2013;110(6):438-43. [View at Publisher] [DOI] [PMID] [Google Scholar]
26. Nam YH, Lee SK. Comparison between skin prick test and serum immunoglobulin E by CAP system to inhalant allergens. Ann Allergy Asthma Immunol. 2017;118(5):608-13. [View at Publisher] [DOI] [PMID] [Google Scholar]
27. Cho JH, Suh JD, Kim JK, Hong SC, Park IH, Lee HM. Correlation between Skin-prick Testing, Individual Specific IgE Tests, and a Multiallergen IgE Assay for Allergy Detection in Patients with Chronic Rhinitis. Am J Rhinol Allergy. 2014;28(5):388-91. [View at Publisher] [DOI] [PMID] [Google Scholar]
28. Calabria CW, Dietrich J, Hagan L. Comparison of serum-specific IgE (ImmunoCAP) and skin-prick test results for 53 inhalant allergens in patients with chronic rhinitis. Allergy Asthma proc. 2009;30(4):386-96. [View at Publisher] [DOI] [PMID] [Google Scholar]
29. Jiang X-D, Li G-Y, Dong Z, Zhu D-D. Correlation Analysis of two Serum-specific Immunoglobulin E Test Systems and Skin-Prick Test in Allergic Rhinitis Patients from Northeast China. Am J Rhinol Allergy. 2011;25(2):116-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
30. Asha'ari ZA, Suhaimi Y, Yusof RA, Rushdan I, Maraina CH. Comparison of serum specific IgE with skin prick test in the diagnosis of allergy in Malaysia. Med J Malaysia. 2011;66(3):202-6. [View at Publisher] [Google Scholar] [PMID] [View at Publisher] [PMID] [Google Scholar]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.