Volume 16, Issue 2 (Mar-Apr 2022)                   mljgoums 2022, 16(2): 48-55 | Back to browse issues page

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Janitermi M, fattahi E, Jorsaraei S G A. Effect of Pre-Incubation of Electrospun Silk Fibroin Scaffold in Complete and Serum-Free Media on Survival and Proliferation of Rat Bone Marrow Mesenchymal Stem Cells. mljgoums. 2022; 16 (2) :48-55
URL: http://mlj.goums.ac.ir/article-1-1373-en.html
1- Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , esmail_fattahy@yahoo.com
3- Fatemeh Zahra Infertility and Reproductive Health Research Centre, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Abstract:   (318 Views)
Background and objectives: Developing scaffolds is important for tissue engineering and repairing damaged tissues. The present study aimed to investigate effects of pre-incubation of an electrospun silk fibroin scaffold in complete and serum-free media on proliferation and survival of cells seeded on the scaffold.
Methods: After removing sericin from the silk cocoon and preparing the fibroin solution (3% w/v), the electrospun silk fibroin scaffold was fabricated and its morphology was evaluated by scanning electron microscopy. The scaffolds were pre-incubated in complete and serum-free Dulbecco's Modified Eagle media for one hour (short-term) and 10 days (long-term), and the hydrophilicity of scaffolds was evaluated by measuring the water contact angle. Rat bone marrow mesenchymal stem cells were seeded onto the scaffolds, and cell survival and genomic DNA concentration were evaluated after 21 days.
Results: The short-time pre-incubation of electrospun silk fibroin scaffolds in the complete medium increased the proliferation of seeded cells because of serum protein adsorption. In addition, long-term pre-incubation of the scaffolds in the complete and serum-free media increased cell proliferation due to the increased hydrophilicity of the scaffold (p<0.05). However, only long-term pre-incubation of the scaffolds in the complete medium had a significant effect on cell survival.
Conclusion: The results demonstrated that long-term pre-incubation of the scaffolds in the complete medium have more profound positive effects on cell survival and proliferation compared to short-term pre-incubation.
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Research Article: Research Article | Subject: Others
Received: 2021/03/21 | Accepted: 2021/06/21 | Published: 2022/03/7 | ePublished: 2022/03/7

1. Zhang Y, Fan W, Ma Z, Wu C, Fang W, Liu G, et al. The effects of pore architecture in silk fibroin scaffolds on the growth and differentiation of mesenchymal stem cells expressing BMP7. Acta biomaterialia. 2010; 6(8): 3021-8. [View at Publisher] [DOI:10.1016/j.actbio.2010.02.030] [PubMed] [Google Scholar]
2. Zhao Z, Fan C, Chen F, Sun Y, Xia Y, Ji A, et al. Progress in articular cartilage tissue engineering: a review on therapeutic cells and macromolecular scaffolds. Macromolecular bioscience. 2020; 20(2): 1900278. [View at Publisher] [DOI:10.1002/mabi.201900278] [PubMed] [Google Scholar]
3. Khorshidi S, Solouk A, Mirzadeh H, Mazinani S, Lagaron JM, Sharifi S, et al. A review of key challenges of electrospun scaffolds for tissue‐engineering applications. Journal of tissue engineering and regenerative medicine. 2016; 10(9): 715-38. [View at Publisher] [DOI:10.1002/term.1978] [PubMed] [Google Scholar]
4. Jun I, Han H-S, Edwards JR, Jeon H. Electrospun fibrous scaffolds for tissue engineering: Viewpoints on architecture and fabrication. International journal of molecular sciences. 2018; 19(3): 745. [View at Publisher] [DOI:10.3390/ijms19030745] [PubMed] [Google Scholar]
5. Tan GZ, Zhou Y. Electrospinning of biomimetic fibrous scaffolds for tissue engineering: a review. International Journal of Polymeric Materials and Polymeric Biomaterials. 2020; 69(15): 947-60. [View at Publisher] [DOI:10.1080/00914037.2019.1636248] [Google Scholar]
6. Teixeira MA, Amorim MTP, Felgueiras HP. Poly (vinyl alcohol)-based nanofibrous electrospun scaffolds for tissue engineering applications. Polymers. 2020; 12(1): 7. [View at Publisher] [DOI:10.3390/polym12010007] [PubMed] [Google Scholar]
7. Toffoli A, Parisi L, Bianchi MG, Lumetti S, Bussolati O, Macaluso GM. Thermal treatment to increase titanium wettability induces selective proteins adsorption from blood serum thus affecting osteoblasts adhesion. Materials Science and Engineering: C. 2020;107:110250. [View at Publisher] [DOI:10.1016/j.msec.2019.110250] [PubMed] [Google Scholar]
8. Wilson CJ, Clegg RE, Leavesley DI, Pearcy MJ. Mediation of biomaterial-cell interactions by adsorbed proteins: a review. Tissue engineering. 2005;11(1-2):1-18. [View at Publisher] [DOI:10.1089/ten.2005.11.1] [Google Scholar]
9. Hayrapetyan L, Sarvazyan N. Extracellular Matrix and Adhesion Molecules. Tissue Engineering: Springer. 2020; 29-38. [View at Publisher] [DOI:10.1007/978-3-030-39698-5_3] [Google Scholar]
10. Soriano‐Jerez Y, López‐Rosales L, Cerón‐García MC, Sánchez‐Mirón A, Gallardo‐Rodríguez J, García‐Camacho F, et al. Long‐term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios. Biotechnology and Bioengineering. 2021; 118(3): 1152-65. [View at Publisher] [DOI:10.1002/bit.27632] [PubMed] [Google Scholar]
11. Zelzer M, Albutt D, Alexander MR, Russell NA. The role of albumin and fibronectin in the adhesion of fibroblasts to plasma polymer surfaces. Plasma Processes and Polymers. 2012; 9(2): 149-56. [View at Publisher] [DOI:10.1002/ppap.201100054] [Google Scholar]
12. Noh H, Vogler EA. Volumetric interpretation of protein adsorption: competition from mixtures and the Vroman effect. Biomaterials. 2007; 28(3): 405-22. [View at Publisher] [DOI:10.1016/j.biomaterials.2006.09.006] [PubMed] [Google Scholar]
13. Schmidt DR, Waldeck H, Kao WJ. Protein adsorption to biomaterials. Biological interactions on materials surfaces: Springer; 2009. p. 1-18. [View at Publisher] [DOI:10.1007/978-0-387-98161-1_1] [Google Scholar]
14. Yildirim ED, Besunder R, Pappas D, Allen F, Güçeri S, Sun W. Accelerated differentiation of osteoblast cells on polycaprolactone scaffolds driven by a combined effect of protein coating and plasma modification. Biofabrication. 2010 ;2(1):014109. [DOI:10.1088/1758-5082/2/1/014109] [PubMed] [Google Scholar]
15. Muzzio NE, Pasquale MA, Rios X, Azzaroni O, Llop J, Moya SE. Adsorption and exchangeability of fibronectin and serum albumin protein corona on annealed polyelectrolyte multilayers and their consequences on cell adhesion. Advanced Materials Interfaces. 2019;6(8):1900008. [View at Publisher] [DOI:10.1002/admi.201900008] [Google Scholar]
16. Woo KM, Seo J, Zhang R, Ma PX. Suppression of apoptosis by enhanced protein adsorption on polymer/hydroxyapatite composite scaffolds. Biomaterials. 2007 ;28(16):2622-30. [View at Publisher] [DOI:10.1016/j.biomaterials.2007.02.004] [PubMed] [Google Scholar]
17. Amirikia M, Shariatzadeh SMA, Jorsaraei SGA, Soleimani Mehranjani M. Impact of pre-incubation time of silk fibroin scaffolds in culture medium on cell proliferation and attachment. Tissue Cell. 2017 ;49(6):657-663. [View at Publisher] [DOI:10.1016/j.tice.2017.09.002] [PubMed] [Google Scholar]
18. Kosik-Kozioł A, Graham E, Jaroszewicz J, Chlanda A, Kumar PTS, Ivanovski S, et al. Surface Modification of 3D Printed Polycaprolactone Constructs via a Solvent Treatment: Impact on Physical and Osteogenic Properties. ACS Biomater Sci Eng. 2019 14;5(1):318-328. [DOI:10.1021/acsbiomaterials.8b01018] [PubMed] [Google Scholar]
19. Chen RI, Gallant ND, Smith JR, Kipper MJ, Simon CG Jr. Time-dependent effects of pre-aging polymer films in cell culture medium on cell adhesion and spreading. J Mater Sci Mater Med. 2008 ;19(4):1759-66. [View at Publisher] [DOI:10.1007/s10856-007-3309-x] [PubMed] [Google Scholar]
20. Chatterjee K, Hung S, Kumar G, Simon CG. Time-Dependent Effects of Pre-Aging 3D Polymer Scaffolds in Cell Culture Medium on Cell Proliferation. J Funct Biomater. 2012 22;3(2):372-81. [DOI:10.3390/jfb3020372] [PubMed] [Google Scholar]
21. Rockwood DN, Preda RC, Yücel T, Wang X, Lovett ML, Kaplan DL. Materials fabrication from Bombyx mori silk fibroin. Nature protocols. 2011;6(10):1612. [DOI:10.1038/nprot.2011.379] [PubMed] [Google Scholar]
22. Zhang K, Mo X, Huang C, He C, Wang H. Electrospun scaffolds from silk fibroin and their cellular compatibility. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials. 2010;93(3):976-83. [View at Publisher] [Google Scholar]
23. Bhardwaj N, Kundu SC. Chondrogenic differentiation of rat MSCs on porous scaffolds of silk fibroin/chitosan blends. Biomaterials. 2012 ;33(10):2848-57. [View at Publisher] [DOI:10.1016/j.biomaterials.2011.12.028] [PubMed] [Google Scholar]
24. Barlian A, Judawisastra H, Alfarafisa NM, Wibowo UA, Rosadi I. Chondrogenic differentiation of adipose-derived mesenchymal stem cells induced by L-ascorbic acid and platelet rich plasma on silk fibroin scaffold. PeerJ. 2018 19;6:e5809. [View at Publisher] [DOI:10.7717/peerj.5809] [PubMed] [Google Scholar]
25. Forsey RW, Chaudhuri JB. Validity of DNA analysis to determine cell numbers in tissue engineering scaffolds. Biotechnol Lett. 2009 ;31(6):819-23. [View at Publisher] [DOI:10.1007/s10529-009-9940-5] [PubMed] [Google Scholar]
26. Qi Y, Wang H, Wei K, Yang Y, Zheng RY, Kim IS, et al. A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures. Int J Mol Sci. 2017 3;18(3):237. [View at Publisher] [DOI:10.3390/ijms18030237] [PubMed] [Google Scholar]
27. Janitermi M, Jorsarai SGA, Fattahi E. Chondrogenic Differentiation of Mesenchymal Stem Cells from Rat Bone Marrow on the Elastic Modulus of Electrospun Silk Fibroin Scaffolds. Regenerative Engineering and Translational Medicine. 2021:1-9. [View at Publisher] [DOI:10.1007/s40883-021-00199-x] [Google Scholar]
28. Li DW, He J, He FL, Liu YL, Liu YY, Ye YJ, et al. Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells. J Biomater Appl. 2018 ;32(9):1164-1173. [View at Publisher] [DOI:10.1177/0885328218757767] [PubMed] [Google Scholar]
29. Wei J, Igarashi T, Okumori N, Igarashi T, Maetani T, Liu B, et al. Influence of surface wettability on competitive protein adsorption and initial attachment of osteoblasts. Biomed Mater. 2009 ;4(4):045002. [View at Publisher] [DOI:10.1088/1748-6041/4/4/045002] [PubMed] [Google Scholar]
30. Chen L, Hu JS, Xu JL, Shao CL, Wang GY. Biological and Chemical Diversity of Ascidian-Associated Microorganisms. Mar Drugs. 2018 1;16(10):362. [View at Publisher] [DOI:10.3390/md16100362] [PubMed] [Google Scholar]

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