Volume 15, Issue 3 (May-Jun 2021)                   mljgoums 2021, 15(3): 1-6 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Fallah M, Shiri A, Maghsood A H, Matini M. Comparison of Biochemical Compounds of Fertile and Infertile Hydatid Cyst Fluid of Animaland Human Origin. mljgoums. 2021; 15 (3) :1-6
URL: http://mlj.goums.ac.ir/article-1-1284-en.html
1- Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , fallah@umsha.ac.ir
2- Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3- Associate Professor, Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Abstract:   (649 Views)
Background and objectives: Hydatidosis is an important zoonotic disease with widespread distribution. For unknown reasons, some cysts are unable to produce protoscoleces, and little is known about the mechanisms involved in infertile cyst production. Therefore, characterization of hydatid cyst fluid (HCF) components could help clarify the host-parasite relationship and the fertility process of cyst. The aim of this study was to identify and quantify biochemical components of HCF of fertile and infertile cysts from different hosts.
Methods: A total of 35 HCF samples were obtained from the liver and lung of 16 cattle, 16 sheep and three humans. Fertility of cysts was determined by examination of cysts’ content. Then, total fluid was aspirated aseptically from each cyst. The samples were centrifuged at 10000×g for 15 min at 4°C, and then concentrated and dialyzed against phosphate buffer saline using an Amicon Ultra-15 5000 MWCO centrifugal filter device (Millipore, USA). All biochemical components were quantified by an automatic analyzer.
Results: The value of lactate dehydrogenase, potassium, calcium, cholesterol, glucose, urea and uric acid differed significantly in cysts collected from different hosts (P<0.001). There was also a significant difference in the amount of lactate dehydrogenase, aspartate aminotransferase and cholesterol between fertile and infertile cysts (P<0.039).
Conclusion: Due to the differences in biochemical composition of HCF of different host origin, it is possible that the host plays a crucial role in determining the type of biochemistry in hydatid cyst as well as in hydatid cyst fertility.
Full-Text [PDF 626 kb]   (213 Downloads) |   |   Full-Text (HTML)  (198 Views)  
Research Article: Original Paper | Subject: Parasitology
Received: 2020/02/22 | Accepted: 2020/05/31 | Published: 2021/04/28 | ePublished: 2021/04/28

References
1. Thompson RC. Biology and systematics of Echinococcus. In: Thompson RCA, Deplazes P, Lymbery A (Eds). Echinoccocus and Echinoccocosis; Advances in Parasitology. Chapter 2: 2017; 95: 65-109 [DOI:10.1016/bs.apar.2016.07.001] [Google Scholar]
2. Juyi L, Yan J, Xiufang W, Zhaoqing Z, Junliang L, Mingxing Z, Wei Z. Analysis of the chemical components of hydatid fluid from Echinococcus granulosus. Rev Soc Bras Med Trop. 2013; 46(5): 605-10. [DOI:10.1590/0037-8682-0154-2013] [PubMed] [Google Scholar]
3. Eckert J, Thompson RC. Historical aspects of echinococcosis. In: Thompson RCA, Deplazes P, Lymbery A (Eds). Echinoccocus and Echinoccocosis; Part A. Advances in Parasitology. 2017; 95: 1-64 [DOI:10.1016/bs.apar.2016.07.003] [Google Scholar]
4. Manterola C, Vial M, Melo A, Oberg C, Fonseca F. Viability and fertility of human hepatic hydatid cysts. World J Surg. 2006; 30(2): 227-32. [DOI:10.1007/s00268-005-0215-6] [Google Scholar]
5. Roberts L, Janovy J, Nadler S. Foundations in Parasitology. 9th ed. New York, McGraw-Hill. 2013. [PubMed] [Google Scholar]
6. Pakala T, Molina M, Wu GY. Hepatic echinococcal cysts: a review. Journal of Clinical and Translational Hepatology. 2016; 4: 39-46. [DOI:10.14218/JCTH.2015.00036] [PubMed] [Google Scholar]
7. Sharif M, Keyghobadi M, Ziaei H, Izadi J, Gholami S, Khaliliyan A. Measurement of Biochemical Components of Liver Hydatid Cyst Fluids in Human, Sheep, Goat, Cattle and Camel Mazandaran 2004. J Arak Uni Med Sci. 2005; 8 (2) :24-31.[Persian] [View at Publisher] [Google Scholar]
8. Cirenei A, Bertoldi I. Evolution of surgery for liver hydatidosis from 1950 to today: analysis of a personal experience. World Journal of Surgery. 2001; 25(1): 87-92 [DOI:10.1007/s002680020368] [Google Scholar]
9. Bayne A, Hendricksl L, Beckerdi F, Harkemar R. Comparisons of lactate dehydrogenase in several trematodes and a host tissue. Compar Biochem Physiol. 1968; 24: 275-278. [DOI:10.1016/0010-406X(68)90975-4] [PubMed] [Google Scholar]
10. Mcmanus DP, Smyth JD. Differences in the chemical composition and carbohydrate metabolism of Echinococcus granulosus (horse and sheep strains) and E. multilocularis. Parasitology. 1978; 77: 103-109. [DOI:10.1017/S0031182000048769] [PubMed] [Google Scholar]
11. Frayha GJ, Haddad R. Comparative chemical composition of protoscoleces and hydatid cyst fluid of Echinococcus granulosus (Cestoda). Int J Parasitol. 1980; 10(5-6):359-64 [DOI:10.1016/0020-7519(80)90036-3] [PubMed] [Google Scholar]
12. Shaldoum FM,Ahmed WF, Hamza HT, Shahin MS. Comparative Study on Copper, Zinc, Magnesium and Iron in Hydatid Cyst Fluid (Supernatant and Residue) in Sheep and Camel in Egypt. The Egypt J Hosp Med 2017; 66: 40- 45 [DOI:10.12816/0034631] [Google Scholar]
13. Chowdhury N, Singh R. Distribution of some elements in hydatid cysts of Echinococcus granulosus from buffalo (Bubalusbubalis). J Helminthol. 1993; 67(02):112-4 [DOI:10.1017/S0022149X00012980] [PubMed] [Google Scholar]
14. Shaafie IA, Khan AH, Rambabu K. Biochemical profiles of hydatid cyst fluids of Echinococcusranulosus of human and animal origin in Libya. J Helminthol. 1999; 73(03): 255-8. [DOI:10.1017/S0022149X99000402] [PubMed]
15. Brehm K, Koziol U. Echinococcus-Host Interactions at Cellular and Molecular Levels. Adv Parasitol. 2017;95:147-212. [DOI:10.1016/bs.apar.2016.09.001] [PubMed] [Google Scholar]
16. Vatankhah A, Rouhani S. A Quantitative Study of Enzyme Levels in the Fluid of Sterile and Fertile Echinococcusgranulosus Cysts in Sheep. J Sch Pub HelthInst Pub Helth Res. 2003; 1(3):49-56. [View at Publisher] [Google Scholar]
17. Muhsin SS, Mohsin MIR, Hossien YK. Analysis of Chemical Component of Hydatid fluid in infected sheep with Echinecoccus granulosus. IOSR Journal of Nursing and Health Science. 2015; 4( 6 Ver. I): 64-67 [Google Scholar]
18. Monteiro KM, deCarvalho MO, Zaha A, Ferreira HB. Proteomic analysis of the Echinococcusgranulosusmetacestode during infection of its intermediate host. Proteomics. 2010; 10(10): 1985-99 [DOI:10.1002/pmic.200900506] [PubMed] [Google Scholar]
19. Aziz A, Zhang W, Li J, Loukas A, McManus DP, Mulvenna J. Proteomic characterization of Echinococcus granulosus hydatid cyst fluid from sheep, cattle and humans. Journal of Proteomics 2011; 74(9): 1560-72. [DOI:10.1016/j.jprot.2011.02.021] [PubMed] [Google Scholar]
20. Eslami-rad Z, Dalimi-asl A, Mobedi E. Comparison of protein and lipids level in hydatid fluid of sheep, goat and cattle. Journal of Inflammatory Diseases. 1999; 3 (3) :75-79. [Persian] [View at Publisher] [Google Scholar]
21. Ahn CS, Han X, Bae YA, Ma X, Kim JT, Cai H, et al . Alteration of immunoproteome profile of Echinococcus granulosus hydatid fluid with progression of cystic echinococcosis. Parasites & Vectors. 2015; 8(10): 1-10. [DOI:10.1186/s13071-014-0610-7] [PubMed] [Google Scholar]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.