Volume 16, Issue 1 (Jan-Feb 2022)                   mljgoums 2022, 16(1): 40-47 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

zanghaneh F, farzanegi P, asgharpour H. Effect of Exercise Training and Atorvastatin Supplementation on Beclin1, LC3-I and LC3-П Expression in Old Diabetic Rats. mljgoums 2022; 16 (1) :40-47
URL: http://mlj.goums.ac.ir/article-1-1275-en.html
1- Department of Exercise Physiology, Aliabad Katul Branch, Islamic Azad University, Aliabad Katul, Iran
2- Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran , parvin.farzanegi@gmail.com
Abstract:   (1425 Views)
Background and objectives: Programmed autophagy is a genetically and evolutionarily conserved process that destroys long-lived cellular proteins and organelles. This study aimed to investigate effects of continuous and interval exercise training with or without atorvastatin supplementation on Beclin1, LC3-I and LC3-П expression in old rats with type 2 diabetes.
Methods: Sixty three male Wistar rats were divided into eight groups. Continuous exercise was performed at a speed of 15-29 m/min for 5-22 minutes. Interval exercise program consisted of six 2.5-minute sets that included a four-minute rest period between each set. The rats in the supplementation groups also received 20 mg/kg body weight atorvastatin daily via intraperitoneal injection. At the end of the training period, the expression of Beclin1, LC3-I and LC3-П in soleus muscle was measured by RT-PCR. One-way ANOVA was used for data analysis at statistical significance of 0.05.
Results: The results showed that both exercise trainings with or without atorvastatin significantly reduced LC3I, LC3-II and Beclin1 compared with the diabetic control group (P<0.05). In addition, the effects of the trainings and atorvastatin supplement did not differ significantly (P>0.05).
Conclusion: The results indicate that continuous and interval exercise program alone and combined with atorvastatin supplementation could significantly reduce LC3-1, LC3-II and Beclin1 level in soleus muscle of old diabetic rats.
Full-Text [PDF 966 kb]   (470 Downloads) |   |   Full-Text (HTML)  (332 Views)  
Research Article: Original Paper | Subject: Sport Physiology
Received: 2019/12/17 | Accepted: 2020/01/15 | Published: 2021/12/29 | ePublished: 2021/12/29

1. Paredes-López O, Cervantes-Ceja ML, Vigna-Pérez M, Hernández-Pérez T. Berries: improving human health and healthy aging, and promoting quality life-a review. Plant Foods Hum Nutr 2010;65(3):299-308. [View at Publisher] [DOI:10.1007/s11130-010-0177-1] [PubMed] [Google Scholar]
2. Habibi A, Nemadi-Vosoughi M, Habibi S, Mohammadi M. Quality of life and prevalence of chronic illnesses among elderly people: A cross-sectional survey. J Health. 2012; 3(1): 58-66. [View at Publisher] [Google Scholar]
3. Gong Z, Muzumdar RH. Pancreatic function, type 2 diabetes, and metabolism in aging. Inter J endocrinol. 2012;2012. [View at Publisher] [DOI:10.1155/2012/320482] [PubMed] [Google Scholar]
4. Duntas L,Orgiazzi J , Brabant G.The interface between Thyroid and Diabetes mellitus. clin Endocrinol. 2011; 75: 1-9. [View at Publisher] [DOI:10.1111/j.1365-2265.2011.04029.x] [Google Scholar]
5. Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care. 2004;27:1047-1053. [View at Publisher] [DOI:10.2337/diacare.27.5.1047] [Google Scholar]
6. Saini V. Molecular mechanisms of insulin resistance in type 2 diabetes mellitus. World J diabetes. 2010; 1(3): 68. [DOI:10.4239/wjd.v1.i3.68] [PubMed] [Google Scholar]
7. Pasiakos SM, Carbone JW. Assessment of skeletal muscle proteolysis and the regulatory response to nutrition and exercise. IUBMB life. 2014; 66(7): 478-84. [View at Publisher] [DOI:10.1002/iub.1291] [PubMed] [Google Scholar]
8. Pagano AF, Py G, Bernardi H, Candau RB, Sanchez AM. Autophagy and protein turnover signaling in slow-twitch muscle during exercise. Med Sci Spor Exe. 2014; 46(7): 1314-25. [DOI:10.1249/MSS.0000000000000237] [PubMed] [Google Scholar]
9. Islam M, Sooro M, Zhang P. Autophagic regulation of p62 is critical for cancer therapy. InterJ Mol Sci. 2018; 19(5): 1405. [View at Publisher] [DOI:10.3390/ijms19051405] [PubMed] [Google Scholar]
10. Wirawan E, Lippens S, Vanden Berghe T, Romagnoli A, Fimia GM, Piacentini M, et al. Beclin1: a role in membrane dynamics and beyond. Autophagy. 2012;8(1):6-17. [View at Publisher] [DOI:10.4161/auto.8.1.16645] [PubMed] [Google Scholar]
11. Eskelinen E-L, Saftig P. Autophagy: a lysosomal degradation pathway with a central role in health and disease. Biochim Biophys Acta. 2009; 1793(4): 664-73. [View at Publisher] [DOI:10.1016/j.bbamcr.2008.07.014] [PubMed] [Google Scholar]
12. Wang J, Pan X-L, Ding L-J, Liu D-Y, Lei D-P, Jin T. Aberrant expression of Beclin1 and LC3 correlates with poor prognosis of human hypopharyngeal squamous cell carcinoma. PloS one. 2013; 8(7): e69038. [DOI:10.1371/journal.pone.0069038] [PubMed] [Google Scholar]
13. Kang R, Zeh H, Lotze M, Tang D. The Beclin 1 network regulates autophagy and apoptosis. Cell Death Differ. 2011;18(4):571. [View at Publisher] [DOI:10.1038/cdd.2010.191] [PubMed] [Google Scholar]
14. Brandt N, Dethlefsen MM, Bangsbo J, Pilegaard H. PGC-1α and exercise intensity dependent adaptations in mouse skeletal muscle. PloS one 2017;12(10):e0185993. [View at Publisher] [DOI:10.1371/journal.pone.0185993] [PubMed] [Google Scholar]
15. Arboix A, García-Eroles L, Oliveres M, Targa C, Balcells M, Massons J. Pretreatment with statins improves early outcome in patients with first-ever ischaemic stroke: a pleiotropic effect of statins or a beneficial effect of hypercholesterolemia? BMC neurology. 2010;10(1):47. [View at Publisher] [DOI:10.1186/1471-2377-10-47] [PubMed] [Google Scholar]
16. Mahdian H, Farzanegi P, Farzaneh-Hessari A. The effect of combined therapy with resveratrol, and continuous and interval exercises on levels of apoptotic biomarkers in heart tissue of male rats with non-alcoholic fatty liver. Feyz Journal of Kashan University of Medical Sciences. 2018;22(5):469-77. [Persian] [View at Publisher] [Google Scholar]
17. Di Stasi SL, MacLeod TD, Winters JD, Binder-Macleod SA. Effects of statins on skeletal muscle: a perspective for physical therapists. Phys Ther. 2010; 90(10): 1530-42. [DOI:10.2522/ptj.20090251] [PubMed] [Google Scholar]
18. Mozafari M, Pharm D, Ali Akbar Nekooeian, Mohammad Reza Panjeshahin1, Hamid Reza Zare The Effects of Resveratrol in Rats with Simultaneous Type 2 Diabetes and Renal Hypertension: a Study of Antihypertensive Mechanism. Iran J Med Sci March. 2015; 40(2): 152-160. [Persian] [View at Publisher] [DOI:10.1177/1934578X1501000232]
19. Choi DH, Cho JY. Effect of treadmill exercise on skeletal muscle autophagy in rats with obesity induced by a high-fat diet Do Keun Cho1. JENB (Journal of Exercise Nutrition & Biochemistry). 2017; 21(3): 26-34. [DOI:10.20463/jenb.2017.0013] [PubMed] [Google Scholar]
20. Markaki M, Metaxakis A, Tavernarakis N. The role of autophagy in aging: molecular mechanisms. Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging: Elsevier; 2017: 123-38. [View at Publisher] [DOI:10.1016/B978-0-12-812146-7.00002-0] [Google Scholar]
21. Ghahremani M, Azarbaijani M, Piri M, Raoufi A. Effect of frequency aerobic exercise on expression of Bcl-2 and Bax gene in mice with myocardial infarction. Armaghane danesh 2018;22(6):781-91. [Persian]. [View at Publisher] [Google Scholar]
22. Siahkohian M, Asgharpour-arshad M, Bolboli L, Jafari A, Sheikhzadeh hesari F. Effect of 12-WeeksAerobicTraining on Some Indices of Skeletal Muscle Apoptosis in Male Rats. Med J Tabriz Uni Med Sciences Health Services. 2018;39(6):35-43. [Persian]. [View at Publisher] [Google Scholar]
23. Linden MA, Fletcher JA, Morris EM, Meers GM, Laughlin MH, Booth FW, Sowers JR, Ibdah JA, Thyfault JP, Rector RS. Treating NAFLD in OLETF Rats with Vigorous-Intensity Interval Exercise Training. Med Sci Sports Exerc. 2015; 47(3): 556-67. [DOI:10.1249/MSS.0000000000000430] [PubMed] [Google Scholar]
24. Perry BD, Caldow MK, Brennan-Speranza TC, Sbaraglia M, Jerums G, Garnham A, et al. Muscle atrophy in patients with Type 2 Diabetes Mellitus: roles of inflammatory pathways, physical activity and exercise. Exer Immunol Rev. 2016;22:94. [PubMed] [Google Scholar]
25. Guan Z-F, Zhou X-L, Zhang X-M, Zhang Y, Wang Y-M, Guo Q-L, et al. Beclin1-mediated autophagy may be involved in the elderly cognitive and affective disorders in streptozotocin-induced diabetic mice. Transl Neurodegener. 2016; 5(1): 22. [DOI:10.1186/s40035-016-0070-4] [PubMed] [Google Scholar]
26. Sun S, Zhang M, Lin J, Hu J, Zhang R, Li C, et al. Lin28a protects against diabetic cardiomyopathy via the PKA/ROCK2 pathway. Biochem Biophys Res Commun 2016;469(1):29-36. [View at Publisher] [DOI:10.1016/j.bbrc.2015.11.065] [PubMed] [Google Scholar]
27. Grip O, Janciauskiene S, Bredberg A. Use of atorvastatin as an anti‐inflammatory treatment in Crohn's disease. British J pharmacol. 2008;155(7):1085-92. [View at Publisher] [DOI:10.1038/bjp.2008.369] [PubMed] [Google Scholar]
28. Decuypere J-P, Parys JB, Bultynck G. Regulation of the autophagic bcl-2/beclin 1 interaction. Cells. 2012; 1(3): 284-312. [View at Publisher] [DOI:10.3390/cells1030284] [PubMed] [Google Scholar]
29. Do Keun Cho DHC, Cho JY. Effect of treadmill exercise on skeletal muscle autophagy in rats with obesity induced by a high-fat diet. J Exer Nut Biochem. 2017;21(3):26. [DOI:10.20463/jenb.2017.0013] [PubMed] [Google Scholar]
30. He C, Zhu H, Li H, Zou M-H, Xie Z. Dissociation of Bcl-2-Beclin1 complex by activated AMPK enhances cardiac autophagy and protects against cardiomyocyte apoptosis in diabetes. Diabetes. 2013; 62(4): 1270-81. [View at Publisher] [DOI:10.2337/db12-0533] [PubMed] [Google Scholar]
31. Giménez-Xavier P, Francisco R, Platini F, Pérez R, Ambrosio S. LC3-I conversion to LC3-II does not necessarily result in complete autophagy. Inter J Mol Med. 2008;22(6):781-5. [View at Publisher] [PubMed] [Google Scholar]
32. Talaei A, Mahmoudpoor M, Shahdost M . The Effect of Atorvastatin on Inflammatory Markers in Patients with Type Two Diabetes . Journal of Arak University of Medical Sciences 2018; 21 (4) :40-47. [Persian]. [View at Publisher]
33. Mohammadi MT, Ramezani Binabaj M, Mirjalili MH, Mojtaba, Jafari M, Salem F. Effect of atorvastatin on pancreatic oxidative stress in Streptozotocin-induced diabetic rat. Iranian Journal of Endocrinology and Metabolism. 2013;15(2):197-204. [Persian]. [View at Publisher] [Google Scholar]
34. Azamian Jazi A, Haffezi M R, Opera H, Abdi H. The Effect of Endurance Exercise Training and Atorvastatin on VEGF in Rat Following Experimental Myocardial Infarction. sjimu. 2016; 24(4): 21-31. [Persian]. [View at Publisher] [DOI:10.18869/acadpub.sjimu.24.4.21] [Google Scholar]
35. Mejías-Peña Y, Rodriguez-Miguelez P, Fernandez-Gonzalo R, Martínez-Flórez S, Almar M, de Paz JA, et al. Effects of aerobic training on markers of autophagy in the elderly. Age. 2016;38(2):33. [DOI:10.1007/s11357-016-9897-y] [PubMed] [Google Scholar]
36. Mejías-Peña Y, Estébanez B, Rodriguez-Miguelez P, Fernandez-Gonzalo R, Almar M, de Paz JA, et al. Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects. Aging (Albany NY). 2017; 9(2): 408-418. [View at Publisher] [DOI:10.18632/aging.101167] [PubMed] [Google Scholar]
37. Ju J-s, Jeon S-i, Park J-y, Lee J-y, Lee S-c, Cho K-j, et al. Autophagy plays a role in skeletal muscle mitochondrial biogenesis in an endurance exercise-trained condition. J Physiol Sci. 2016; 66(5): 417-30. [View at Publisher] [DOI:10.1007/s12576-016-0440-9] [PubMed] [Google Scholar]
38. Agha-Alinejad H, Hashemi Jokar E. Effect of Six Weeks of Interval Exercise Training along with Selenium Nanoparticle Ingestion on Bcl-2 and LC3 Genes expression in the Tumor Tissue of Breast Tumor-Bearing Mice. ijbd. 2019; 12(2): 26-37.[Persian] [View at Publisher] [DOI:10.30699/acadpub.ijbd.12.2.26] [Google Scholar]
39. Brandt N, Nielsen L, Thiellesen Buch B, Gudiksen A, Ringholm S, Hellsten Y, et al. Impact of β-adrenergic signaling in PGC-1α-mediated adaptations in mouse skeletal muscle. Am J Physiol Endocrinol Metab. 2018; 314(1): E1-E20. [View at Publisher] [DOI:10.1152/ajpendo.00082.2017] [PubMed] [Google Scholar]
40. Akopova O, Kolchinskaya L, Nosar V, Bouryi V, Mankovska I, Sagach V. Cytochrome C as an amplifier of ROS release in mitochondria. Fiziol Zh 2012; 58(1): 3-12. [DOI:10.15407/fz58.01.003] [PubMed] [Google Scholar]
41. Kim YA, Kim YS, Song W. Autophagic response to a single bout of moderate exercise in murine skeletal muscle. J physiol biochem. 2012; 68(2): 229-35. [View at Publisher] [DOI:10.1007/s13105-011-0135-x] [PubMed] [Google Scholar]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.