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mljgoums 2018, 12(6): 19-23 |
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Khaledi A, Fatemeh D, Javad Hosseini S M, Meskini M, Esmaeili D. Antimicrobial Resistance Pattern of Acinetobacter baumannii Strains Isolated from Intensive Care Unit Patients . mljgoums 2018; 12 (6) :19-23
URL: http://mlj.goums.ac.ir/article-1-1129-en.html
URL: http://mlj.goums.ac.ir/article-1-1129-en.html
Azad Khaledi1 
, Daymad Fatemeh2 , Seyyed Mohammad Javad Hosseini3 , Maryam Meskini2 , Davoud Esmaeili 4
, Daymad Fatemeh2 , Seyyed Mohammad Javad Hosseini3 , Maryam Meskini2 , Davoud Esmaeili 4
1- Infectious Diseases Research Center, Kashan University of Medical Science, Kashan, Iran Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Science, Kashan, Iran
2- Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
3- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran , esm114@gmail.com
2- Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
3- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran , esm114@gmail.com
Abstract: (16847 Views)
ABSTRACT
Background and Objectives: Acinetobacter baumannii is an opportunistic pathogen that affects different groups of people, especially intensive care unit (ICU) patients. The prevalence of infections caused by this bacterium is very high. Today, prevalence of infections caused by multidrug-resistant (MDR) and extreme-drug resistant (XDR) strains is increasing. This study aimed to determine the antibiotic susceptibility pattern of A. baumannii isolates from ICU patients.
Methods: This cross-sectional study was conducted from October 2014 to March 2015 on patients admitted to ICU of Imam Khomeini hospital in Tehran, Iran. Clinical samples of various sources were collected from patients. Isolates were detected and identified via microbiological and biochemical tests as well as PCR amplification of the blaOxa51 gene. Then, susceptibility testing was performed using the Kirby-Bauer disk diffusion test. Statistical analysis was performed with SPSS (version 22, Chicago, IL, USA) using Chi-square and Fisher’s exact tests.
Results: Of the total of 62 clinical samples, 24 (39%) were respiratory samples and only three (6%) were cerebrospinal fluid samples. Most MDR and XDR strains were isolated from respiratory samples. The highest resistance rate was against ceftriaxone, ticarcillin and erythromycin (100%), while the lowest resistance rate was against to minocycline (20%).
Conclusion: Owing to detection of high multi-drug resistance isolates in the present study, and importance of multi-drug resistance in A. baumannii, the identification of multi-drug resistance genes and their reporting to health care/treatment centers is important. Thus, it is recommended to perform susceptibility testing to help determine the most effective antibiotic(s) for the treatment of infections in ICU patients.
Keywords: Acinetobacter baumannii, MDR, XDR, ICU.
Background and Objectives: Acinetobacter baumannii is an opportunistic pathogen that affects different groups of people, especially intensive care unit (ICU) patients. The prevalence of infections caused by this bacterium is very high. Today, prevalence of infections caused by multidrug-resistant (MDR) and extreme-drug resistant (XDR) strains is increasing. This study aimed to determine the antibiotic susceptibility pattern of A. baumannii isolates from ICU patients.
Methods: This cross-sectional study was conducted from October 2014 to March 2015 on patients admitted to ICU of Imam Khomeini hospital in Tehran, Iran. Clinical samples of various sources were collected from patients. Isolates were detected and identified via microbiological and biochemical tests as well as PCR amplification of the blaOxa51 gene. Then, susceptibility testing was performed using the Kirby-Bauer disk diffusion test. Statistical analysis was performed with SPSS (version 22, Chicago, IL, USA) using Chi-square and Fisher’s exact tests.
Results: Of the total of 62 clinical samples, 24 (39%) were respiratory samples and only three (6%) were cerebrospinal fluid samples. Most MDR and XDR strains were isolated from respiratory samples. The highest resistance rate was against ceftriaxone, ticarcillin and erythromycin (100%), while the lowest resistance rate was against to minocycline (20%).
Conclusion: Owing to detection of high multi-drug resistance isolates in the present study, and importance of multi-drug resistance in A. baumannii, the identification of multi-drug resistance genes and their reporting to health care/treatment centers is important. Thus, it is recommended to perform susceptibility testing to help determine the most effective antibiotic(s) for the treatment of infections in ICU patients.
Keywords: Acinetobacter baumannii, MDR, XDR, ICU.
Research Article: Original Paper |
Received: 2018/10/29 | Accepted: 2018/10/29 | Published: 2018/10/29 | ePublished: 2018/10/29
Received: 2018/10/29 | Accepted: 2018/10/29 | Published: 2018/10/29 | ePublished: 2018/10/29
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