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mljgoums 2017, 11(6): 23-29 |
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Askari B, Bijeh N, Rashid Lamir A. Effects of 8 Weeks of Resistance Training and IGF-1 Injection on Biochemical Markers of Cancer and Colorectal Structures in Rats. mljgoums 2017; 11 (6) :23-29
URL: http://mlj.goums.ac.ir/article-1-1020-en.html
URL: http://mlj.goums.ac.ir/article-1-1020-en.html
1- Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
2- Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran ,bijeh@ferdowsi.um.ac.ir
2- Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran ,
Abstract: (11833 Views)
ABSTRACT
Background and Objectives: We studied effects of eight weeks of resistance training and IGF-1 injection on serum level of IGF-1, IGFBP-3 and IGF-1/IGFBP-3 ratio in Wistar rats.
Methods: We randomly divided 28 male Wistar rats into four groups of saline-injected control (C), resistance training+saline injection (RS), resistance training+IGF-1 injection (RI) and IGF-1 injection (II). Resistance training protocol consisted of climbing a ladder (three days/week with 5 reps/3 sets) while carrying a weight suspended from the tail for eight weeks. IGF-1 and saline (1.5 µg/kg/day) were injected before and after exercise sessions. Serum IGF-1, IGFBP-3 and IGF-1/IGFBP-3 ratio and morphology of colorectal tissue were evaluated.
Results: Serum IGF-1 level and IGF-1/IGFBP-3 ratio decreased in the RS group compared to the other groups (P< 0.05). Rats in the RS group had higher IGFBP-3 level than those in the II and RI groups (P< 0.05). IGF-1 injection had no effect on morphology of colorectal tissue.
Conclusion: The resistance training reduces IGF-1 and increases IGFBP-3 levels, which might represent a link between resistance training and lower risk of colorectal cancer.
Keywords: Exercise, Colorectal Neoplasms, Insulin-Like Growth Factor-1, Insulin-Like Growth Factor Binding Protein-3.
Background and Objectives: We studied effects of eight weeks of resistance training and IGF-1 injection on serum level of IGF-1, IGFBP-3 and IGF-1/IGFBP-3 ratio in Wistar rats.
Methods: We randomly divided 28 male Wistar rats into four groups of saline-injected control (C), resistance training+saline injection (RS), resistance training+IGF-1 injection (RI) and IGF-1 injection (II). Resistance training protocol consisted of climbing a ladder (three days/week with 5 reps/3 sets) while carrying a weight suspended from the tail for eight weeks. IGF-1 and saline (1.5 µg/kg/day) were injected before and after exercise sessions. Serum IGF-1, IGFBP-3 and IGF-1/IGFBP-3 ratio and morphology of colorectal tissue were evaluated.
Results: Serum IGF-1 level and IGF-1/IGFBP-3 ratio decreased in the RS group compared to the other groups (P< 0.05). Rats in the RS group had higher IGFBP-3 level than those in the II and RI groups (P< 0.05). IGF-1 injection had no effect on morphology of colorectal tissue.
Conclusion: The resistance training reduces IGF-1 and increases IGFBP-3 levels, which might represent a link between resistance training and lower risk of colorectal cancer.
Keywords: Exercise, Colorectal Neoplasms, Insulin-Like Growth Factor-1, Insulin-Like Growth Factor Binding Protein-3.
Keywords: Exercise, Colorectal, Insulin-Like Growth Factor-1, Insulin-Like Growth Factor Binding Protein-3.
Research Article: Original Paper |
Received: 2017/11/20 | Accepted: 2017/11/20 | Published: 2017/11/20 | ePublished: 2017/11/20
Received: 2017/11/20 | Accepted: 2017/11/20 | Published: 2017/11/20 | ePublished: 2017/11/20
References
1. Devin JL, Bolam KA, Jenkins DG, Skinner TL. The influence of exercise on the insulin-like growth factor axis in oncology: physiological basis, current, and future perspectives. Cancer Epidemiology and Prevention Biomarkers. 2015; 25(2): 239-49. doi: 10.1158/1055-9965.EPI-15-0406. [DOI:10.1158/1055-9965.EPI-15-0406]
2. Rosa C, Vilaça-Alves J, Fernandes HM, Saavedra FJ, Pinto RS, dos Reis VM. Order effects of combined strength and endurance training on testosterone, cortisol, growth hormone, and IGF-1 binding protein 3 in concurrently trained men. The Journal of Strength & Conditioning Research. 2015; 29(1): 74-9. doi: 10.1519/JSC.0000000000000610. [DOI:10.1519/JSC.0000000000000610]
3. Sandhu MS, Dunger DB, Giovannucci EL. Insulin, insulin-like growth factor-I (IGF-I), IGF binding proteins, their biologic interactions, and colorectal cancer. Journal of the National Cancer Institute. 2002; 94(13): 972-80. [DOI:10.1093/jnci/94.13.972]
4. Ruiz-Casado A, Martín-Ruiz A, Pérez LM, Provencio M, Fiuza-Luces C, Lucia A. Exercise and the Hallmarks of Cancer. Trends in Cancer. 2017; 3(6): 423-41. [DOI:10.1016/j.trecan.2017.04.007]
5. Brahmkhatri VP, Prasanna C, Atreya HS. Insulin-like growth factor system in cancer: novel targeted therapies. BioMed research international. 2015; 2015: 538019. doi: 10.1155/2015/538019. [DOI:10.1155/2015/538019]
6. Thomas RJ, Kenfield SA, Jimenez A. Exercise-induced biochemical changes and their potential influence on cancer: a scientific review. Br J Sports Med. 2017; 51(8): 640-4. [DOI:10.1136/bjsports-2016-096343]
7. Nishida Y, Matsubara T, Tobina T, Shindo M, Tokuyama K, Tanaka K, et al. Effect of low-intensity aerobic exercise on insulin-like growth factor-I and insulin-like growth factor-binding proteins in healthy men. International journal of endocrinology. 2010;2010(452820). doi: 10.1155/2010/452820. [DOI:10.1155/2010/452820]
8. Kaaks R, Lukanova A. Energy balance and cancer: the role of insulin and insulin-like growth factor-I. Proceedings of the Nutrition Society. 2001; 60(1): 91-106. [DOI:10.1079/PNS200070]
9. Giovannucci E. Insulin, insulin-like growth factors and colon cancer: a review of the evidence. The Journal of nutrition. 2001; 131(11): 3109S-20S. [DOI:10.1093/jn/131.11.3109S]
10. Mannerkorpi K, Landin-Wilhelmsen K, Larsson A, Cider Å, Arodell O, Bjersing JL. Acute effects of physical exercise on the serum insulin-like growth factor system in women with fibromyalgia. BMC musculoskeletal disorders. 2017; 18(1): 37. doi: 10.1186/s12891-017-1402-y. [DOI:10.1186/s12891-017-1402-y]
11. Sellami M, Dhahbi W, Hayes LD, Padulo J, Rhibi F, Djemail H, et al. Combined sprint and resistance training abrogates age differences in somatotropic hormones. PloS one. 2017; 12(8): e0183184. [DOI:10.1371/journal.pone.0183184]
12. Wakai K, Suzuki K, Ito Y, Watanabe Y, Inaba Y, Tajima K, et al. Time spent walking or exercising and blood levels of insulin-like growth factor-I (IGF-I) and IGF-binding protein-3 (IGFBP-3): a large-scale cross-sectional study in the Japan Collaborative Cohort study. Asian Pac J Cancer Prev. 2009; 10(Suppl): 23-7.
13. Parekh N, Roberts CB, Vadiveloo M, Puvananayagam T, Albu JB, Lu-Yao GL. Lifestyle, anthropometric, and obesity-related physiologic determinants of insulin-like growth factor-1 in the Third National Health and Nutrition Examination Survey (1988–1994). Annals of epidemiology. 2010; 20(3):182-93. doi: 10.1016/j.annepidem.
14. Fontana L, Klein S, Holloszy JO. Long-term low-protein, low-calorie diet and endurance exercise modulate metabolic factors associated with cancer risk. The American journal of clinical nutrition. 2006; 84(6): 1456-62. [DOI:10.1093/ajcn/84.6.1456]
15. Borst SE, Vincent KR, Lowenthal DT, Braith RW. Effects of Resistance Training on Insulin‐Like Growth Factor and its Binding Proteins in Men and Women Aged 60 to 85. Journal of the American Geriatrics Society. 2002; 50(5): 884-8. [DOI:10.1046/j.1532-5415.2002.50215.x]
16. Santa Mina D, Connor MK, Alibhai SM, Toren P, Guglietti C, Matthew AG, et al. Exercise effects on adipokines and the IGF axis in men with prostate cancer treated with androgen deprivation: a randomized study. Canadian Urological Association Journal. 2013;7(11-12):E692-8. doi: 10.5489/cuaj.235. [DOI:10.5489/cuaj.235]
17. Irwin ML, Varma K, Alvarez-Reeves M, Cadmus L, Wiley A, Chung GG, et al. Randomized controlled trial of aerobic exercise on insulin and insulin-like growth factors in breast cancer survivors: the Yale Exercise and Survivorship study. Cancer Epidemiology and Prevention Biomarkers. 2009; 18(1): 306-13. doi: 10.1158/1055-9965.
18. Lee DH, Kim JY, Lee MK, Lee C, Min J-H, Jeong DH, et al. Effects of a 12-week home-based exercise program on the level of physical activity, insulin, and cytokines in colorectal cancer survivors: a pilot study. Supportive Care in Cancer. 2013; 21(9): 2537-45. doi: 10.1007/s00520-013-1822-7. [DOI:10.1007/s00520-013-1822-7]
19. Koziris L, Hickson R, Chatterton R, Groseth R, Christie J, Goldflies D, et al. Serum levels of total and free IGF-I and IGFBP-3 are increased and maintained in long-term training. Journal of Applied Physiology. 1999; 86(4): 1436-42. [DOI:10.1152/jappl.1999.86.4.1436]
20. Shiratsuchi I, Akagi Y, Kawahara A, Kinugasa T, Romeo K, Yoshida T, et al. Expression of IGF-1 and IGF-1R and their relation to clinicopathological factors in colorectal cancer. Anticancer research. 2011; 31(7): 2541-5.
21. Lee S, Farrar RP. Resistance training induces muscle-specific changes in muscle mass and function in rat. Journal of Exercise physiology online. 2003; 6(2): 80-7.
22. Karbasi S, Zaeemi M, Mohri M, Rashidlamir A, Moosavi Z. Effects of testosterone enanthate and resistance training on myocardium in Wistar rats; clinical and anatomical pathology. Andrologia. 2017; e12908. doi: 10.1111/and.12908. [DOI:10.1111/and.12908]
23. Adams GR, McCue SA. Localized injection of IGF-I results in skeletal muscle hypertrophy in rats. Journal of Applied Physiology. 1998;84(5):1716-22. [DOI:10.1152/jappl.1998.84.5.1716]
24. Rashidlamir A, Hosseini SRA, Hejazi K, Anberani SMM. The effect of eight weeks resistance and aerobic training on myostatin and follistatin expression in cardiac muscle of rats. Journal of cardiovascular and thoracic research. 2016;8(4):164-9. doi: 10.15171/jcvtr.2016.33. [DOI:10.15171/jcvtr.2016.33]
25. Nucci RAB, Teodoro ACdS, Krause Neto W, Silva WdA, de Souza RR, Anaruma CA, et al. Effects of resistance training on liver structure and function of aged rats. The Aging Male. 2017; 11: 1-5. doi: 10.1080/13685538.2017.1350157. [DOI:10.1080/13685538.2017.1350157]
26. Scheett TP, Nemet D, Stoppani J, Maresh CM, Newcomb R, Cooper DM. The effect of endurance-type exercise training on growth mediators and inflammatory cytokines in pre-pubertal and early pubertal males. Pediatric research. 2002; 52(4): 491-7. [DOI:10.1203/00006450-200210000-00006]
27. Meneses-Echávez JF, Jiménez EG, Río-Valle JS, Correa-Bautista JE, Izquierdo M, Ramírez-Vélez R. The insulin-like growth factor system is modulated by exercise in breast cancer survivors: a systematic review and meta-analysis. BMC cancer. 2016; 16(1): 682. [DOI:10.1186/s12885-016-2733-z]
28. Eliakim A, Nemet D. Exercise training, physical fitness and the growth hormone-insulin-like growth factor-1 axis and cytokine balance. Med Sport Sci. 2010; 55: 128-40. doi: 10.1159/000321977. [DOI:10.1159/000321977]
29. Rosario PW. Normal values of serum IGF-1 in adults: results from a Brazilian population. Arq Bras Endocrinol Metabol. 2010; 54(5):477-81. [DOI:10.1590/S0004-27302010000500008]
30. Veldhuis JD, Roelfsema F, Keenan DM, Pincus S. Gender, age, body mass index, and IGF-I individually and jointly determine distinct GH dynamics: analyses in one hundred healthy adults. The Journal of Clinical Endocrinology & Metabolism. 2011;96(1):115-21. doi: 10.1210/jc.2010-1669. [DOI:10.1210/jc.2010-1669]
31. Shanmugalingam T, Bosco C, Ridley AJ, Van Hemelrijck M. Is there a role for IGF‐1 in the development of second primary cancers? Cancer medicine. 2016;5(11):3353-67. doi: 10.1002/cam4.871. [DOI:10.1002/cam4.871]
32. Jacob R, Barrett E, Plewe G, Fagin KD, Sherwin RS. Acute effects of insulin-like growth factor I on glucose and amino acid metabolism in the awake fasted rat. Comparison with insulin. Journal of Clinical Investigation. 1989; 83(5): 1717-23. [DOI:10.1172/JCI114072]
33. Jones JI, Clemmons DR. Insulin-like growth factors and their binding proteins: biological actions. Endocrine reviews. 1995; 16(1): 3-34.
34. Rosendal L, Langberg H, Flyvbjerg A, Frystyk J, Ørskov H, Kjær M. Physical capacity influences the response of insulin-like growth factor and its binding proteins to training. Journal of Applied Physiology. 2002; 93(5): 1669-75. [DOI:10.1152/japplphysiol.00145.2002]
35. Schmitz KH, Ahmed RL, Hannan PJ, Yee D. Safety and efficacy of weight training in recent breast cancer survivors to alter body composition, insulin, and insulin-like growth factor axis proteins. Cancer Epidemiology and Prevention Biomarkers. 2005; 14(7): 1672-80. [DOI:10.1158/1055-9965.EPI-04-0736]
36. Eliakim A, Brasel JA, Mohan S, Wong WLT, Cooper DM. Increased physical activity and the growth hormone-IGF-I axis in adolescent males. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 1998; 275(1): R308-R14. [DOI:10.1152/ajpregu.1998.275.1.R308]
37. Maddalozzo G, Snow C. High intensity resistance training: effects on bone in older men and women. Calcified tissue international. 2000; 66(6): 399-404. [DOI:10.1007/s002230010081]
38. Herndon DN, Ramzy PI, DebRoy MA, Zheng M, Ferrando AA, Chinkes DL, et al. Muscle protein catabolism after severe burn: effects of IGF-1/IGFBP-3 treatment. Annals of surgery. 1999; 229(5): 713-20. [DOI:10.1097/00000658-199905000-00014]
39. Holt R, Sönksen P. Growth hormone, IGF‐I and insulin and their abuse in sport. British journal of pharmacology. 2008; 154(3): 542-56. doi: 10.1038/bjp.2008.99. [DOI:10.1038/bjp.2008.99]
40. Gunter MJ, Leitzmann MF. Obesity and colorectal cancer: epidemiology, mechanisms and candidate genes. The Journal of nutritional biochemistry. 2006;17(3):145-56. [DOI:10.1016/j.jnutbio.2005.06.011]
41. Nomura AM, Stemmermann GN, Lee J, Pollak MN. Serum insulin-like growth factor I and subsequent risk of colorectal cancer among Japanese-American men. American journal of epidemiology. 2003; 158(5): 424-31. [DOI:10.1093/aje/kwg176]
42. Kari F, Dunn S, French J, Barrett J. Roles for insulin-like growth factor-1 in mediating the anti-carcinogenic effects of caloric restriction. The journal of nutrition, health & aging. 1999; 3(2): 92-101.
43. Butler AA, Blakesley VA, Tsokos M, Pouliki V, Wood TL, LeRoith D. Stimulation of tumor growth by recombinant human insulin-like growth factor-I (IGF-I) is dependent on the dose and the level of IGF-I receptor expression. Cancer research. 1998; 58(14): 3021-7.
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