Abstract

Background and Objective: Propionibacterium acne is one of the main causes of acne. Due to the spread of drug resistance, it  is not responsive to  treatment.  This study aimed to determine antibiotic sensitivity of strains of the Propionibacterium acne.

Material and Methods: seventy samples of acne lesions were collected to study the presence of Propionibacterium acne. Microbial Culture technique was used to detect and identify Propionibacterium acne. Antibiotic resistance of the isolates to the antibiotics of Doxycyclin, Azithromycin, Erythromycin, Tetracycline, Clindamycin and Trimethoprim sulfamethoxazole was studied by Antibiogram method.

Results: Of 70 samples, 14 (20%) were positive for Propionibacterium acne.  The results of phenotypic test were confirmed using molecular method. Rate of resistance to   Azithromycin and Erythromycin (50%), Clindamycin (35.71%), Trimethoprim sulfamethoxazole (28.57%), Doxycycline and Tetracycline (14.29%) was determined.

Conclusion: Outbreak of antibiotic resistance to Azithromycin, Erythromycin, and Clindamycin is high. Since the Propionibacterium acne is sensitive to Doxycycline and Trimethoprim Sulfamethoxazole, it is recommended using them to treat acne.

Keywords: Antibiotic susceptibility, Propionibacterium Acne, Ance Protein.

Abstract

Background and Objective: This study aimed to investigate the antimicrobial susceptibility of the most common pathogens in hospitalized neonates in Intensive Care Unit.

Material and Methods: In this one-year descriptive study, 150 blood samples of neonates in Intensive Care Unit of Bahrami hospital of Tehran were divided into two groups of early onset sepsis (the first 72 hours of life) and   late onset sepsis (after the 72 hours of life). After isolating and identifying of bacteria, their antibiotics susceptibility was studied by Kirby- bauer method in accordance with CLSI guidelines.

Result: The most isolated organisms were Klebsiella pneumoniae (41.3%).  Coagulase negative staphylococcus was the cause of early onset septicemia and Klebsiella pneumoniae of both early and late onset septicemia. The highest susceptibility in gram-negative microorganisms was shown to Ciprofloxacin (89.6%) and in gram positive to Vancomycin (82.8%).

Conclusion: Gram-negative bacteria are the main cause of contamination in NICU and ciprofloxacin is the most effective antibiotic. Thus, it is imperative that NICU should be extremely controlled.

Keywords: Septicemia; Antibiotic Susceptibility; Neonatal Intensive Care Unit