Back to the articles list | Back to browse issues page

XML Print

Investigating the effect of Melissa officinalis plant extract on pentylenetetrazol-induced seizures in Autistic rat
Fatemeh Piri1 , Hamid Sepehri2
1- Golestan University of Medical Sciences
2- Golestan University of Medical Sciences , hamsep49@yahoo.com
Abstract:   (1046 Views)
Abstract: Autism is a disease that causes problems in speech, performing social skills, as well as repetitive, stereotyped movements and aimless behaviors. 15 to 30 percent of people with autism experience seizures. However, the reported rate of epilepsy in autism varies between 5 and 40%.Melissa officinalis plant is used in Iranian traditional medicine as a sedative, antipyretic, antispasmodic and anticonvulsant.In this research project, the possible effects of the extract of this plant on convulsions induced by PTZ in the autism model of male Wistar rats were investigated.
Methods: Healthy male and female Wistar rats were selected for mating.Female mice received water and normal food until day 12.5 of pregnancy.Then they are divided into 4 groups. 1-control 2-autism group 3-autism group Melissa officinalis extractwith a dose of 100mg/kg.4. Autism group + Melissa officinalis plant extractwith a dose of 200mg/kg. Then, after 2 weeks, pentylenetetorazole was injected PTZ with a dose of 80 mg/kg to all groups of rats, and after that the animals' behaviors will be examined for 60 minutes. Ricin criterion was used to evaluate seizure severity.
Conclusion:The findings of the present study showed that the administration of the Melissa officinalis plant extract to male rats with autism has inhibitory effects on PTZ-induced seizures.
 
Keywords: autism, Melissa officinalis plant, seizure, PTZ
Full-Text [PDF 422 kb]   (241 Downloads)    
Research Article: Research Article | Subject: Others
Received: 2024/11/1 | Accepted: 2025/06/11
References
1. Malow BA. Sleep disorders, epilepsy, and autism. Ment Retard Dev Disabil Res Rev. 2004;10(2):122-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Williams LM. Precision psychiatry: a neural circuit taxonomy for depression and anxiety. Lancet Psychiatry. 2016;3(5):472-80. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Amiet C, Gourfinkel-An I, Bouzamondo A, Tordjman S, Baulac M, Lechat P, et al. Epilepsy in autism is associated with intellectual disability and gender: evidence from a meta-analysis. Biol Psychiatry. 2008;64(7):577-82. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Epi4K Consortium, Epilepsy Phenome/Genome Project. De novo mutations in epileptic encephalopathies. Nature. 2013;501(7466):217-21. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Saemundsen E, Ludvigsson P, Hilmarsdottir I, Rafnsson V. Autism spectrum disorders in children with seizures in the first year of life-a population‐based study. Epilepsia. 2007;48(9):1724-30. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Mandhane SN, Aavula K, Rajamannar T. Timed pentylenetetrazol infusion test: a comparative analysis with sc PTZ and MES models of anticonvulsant screening in mice. Seizure. 2007;16(7):636-44. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Fiske J, Boyle C. Epilepsy and oral care. Dent Update. 2002;29(4):180-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Ghayour M, Behnam-Rassouli M, Ghayour N, Tehranipour M, Kamyabi-Abkooh A. Investigating the anti-epileptic and sedative effects of hydroalcoholic extract of melissa officinalis (Lemon Balm) leaf on pentylenetetrazol induced epileptiform seizures in wistar rat. J Med Plants . 2012;11(41):64-73. [View at Publisher] [DOI] [Google Scholar]
9. Syed FQ, Mirza MB, Elkady AI, Hakeem KR, Alkarim S. An insight of multitudinous and inveterate pharmacological applications of Foeniculum vulgare (fennel). In: Ozturk M, Hakeem KR, editors. Plant and Human Health, Volume 3: Pharmacology and Therapeutic Uses. Cham: Springer; 2019. p. 231-54. [View at Publisher] [DOI] [Google Scholar]
10. Hara Y, Ago Y, Taruta A, Hasebe S, Kawase H, Tanabe W, et al. Risperidone and aripiprazole alleviate prenatal valproic acid-induced abnormalities in behaviors and dendritic spine density in mice. Psychopharmacology (Berl). 2017;234(21):3217-28. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Gorgij E, Komeili GR, Zakeri Z, Ebrahimi S. A Comparative Study on The Anticonvulsive Effect of The Hydroalcholic Extract of The Melissa Officinalis Plant with Phenytoin on Wistar Rat. Findings Science. 2012;8(1). [View at Publisher] [Google Scholar]
12. Del-Bel EA, Oliveira PR, Oliveira JA, Mishra PK, Jobe PC, Garcia-Cairasco N. Anticonvulsant and proconvulsant roles of nitric oxide in experimental epilepsy models. Braz J Med Biol Res. 1997;30(8):971-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Racine RJ. Modification of seizure activity by electrical stimulation, II: motor seizure. Electroencephalogr Clin Neurophysiol. 1972;32(3):281-94. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Zapata-Sudo G, Mendes TC, Kartnaller MA, Fortes TO, Freitas NF, Kaplan MAC, et al. Sedative and anticonvulsant activities of methanol extract of Dorstenia arifolia in mice. J Ethnopharmacol. 2010;130(1):9-12. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Bikjdaouene L, Escames G, Camacho E, León J, Ferrer JM, Espinosa A, et al. Effects of some synthetic kynurenines on brain amino acids and nitric oxide after pentylenetetrazole administration to rats. J Pineal Res. 2004;36(4):267-77. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Urbańska EM, Drelewska E, Borowicz KK, Błaszczak P, Kleinrok Z, Czuczwar SJ. NG-nitro-L-arginine, a nitric oxide synthase inhibitor, and seizure susceptibility in four seizure models in mice. J Neural Transm (Vienna). 1996;103(10):1145-52. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Puig-Lagunes AA, Manzo J, Beltrán-Parrazal L, Morgado-Valle C, Toledo-Cárdenas R, Lopez-Meraz M-L. Pentylenetetrazole-induced seizures in developing rats prenatally exposed to valproic acid. PeerJ. 2016;4:e2709. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Volkmar FR, Nelson DS. Seizure disorders in autism. J Am Acad Child Adolesc Psychiatry. 1990;29(1):127-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Khan GM, Smolders I, Ebinger G, Michotte Y. 2-Chloro-N6-cyclopentyladenosine-elicited attenuation of evoked glutamate release is not sufficient to give complete protection against pilocarpine-induced seizures in rats. Neuropharmacology. 2001;40(5):657-67. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Kennedy DO, Scholey AB. The psychopharmacology of European herbs with cognition-enhancing properties. Curr Pharm Des. 2006;12(35):4613-23. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Buckmaster PS, Zhang GF, Yamawaki R. Axon sprouting in a model of temporal lobe epilepsy creates a predominantly excitatory feedback circuit. J Neurosci. 2002;22(15):6650-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Awad R, Levac D, Cybulska P, Merali Z, Trudeau VL, Arnason JT. Effects of traditionally used anxiolytic botanicals on enzymes of the γ-aminobutyric acid (GABA) system. Can J Physiol Pharmacol. 2007;85(9):933-42. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Buchbauer G, Jirovetz L, Jager W, Plank C, Dietrich H. Fragrance compounds and essential oils with sedative effects upon inhalation. J Pharm Sci. 1993;82(6):660-4. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Akhondzadeh S, Noroozian M, Mohammadi M, Ohadinia S, Jamshidi AH, Khani M. Melissa officinalis extract in the treatment of patients with mild to moderate Alzheimer's disease: a double blind, randomised, placebo controlled trial. J Neurol Neurosurg Psychiatry. 2003;74(7):863-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
25. Ozarowski M, Mikolajczak PL, Piasecka A, Kachlicki P, Kujawski R, Bogacz A, et al. Influence of the Melissa officinalis leaf extract on long‐term memory in scopolamine animal model with assessment of mechanism of action. Evid Based Complement Alternat Med. 2016;2016(1):9729818. [View at Publisher] [DOI] [PMID] [Google Scholar]
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.