Volume 11, Issue 1 (Jan-Feb- 2017 2017)                   mljgoums 2017, 11(1): 28-32 | Back to browse issues page

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Naseri Poor T, Nasrollahnejad S, Shahbazi S, Rahnama K. Optimization of Extracellular Cellulase Production by Trichoderma harzianum. mljgoums. 2017; 11 (1) :28-32
URL: http://mlj.goums.ac.ir/article-1-928-en.html
1- Department of plant protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran , naseripour1391@gmail.com
2- Department of plant protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran
3- Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Alborz, Iran
Abstract:   (7226 Views)
       Background and Objectives: Cellulose is a major component of plant biomass, which can be converted into biofuels and valuable chemicals. The key step in utilization of this organic material is its hydrolysis into soluble sugars. This study evaluated cellulase production by Trichoderma harzianum under different pH values, temperatures and incubation periods with the aim to increase enzyme production and decrease its costs.
Methods: The amount of protein production and the hydrolytic activity of cellulase enzymes including exoglucanase, endoglucanase and β-glucosidase produced by T. harzianum were evaluated using various substrates such as avicel, carboxymethyl cellulose, cellobiose, Whatman grade 1 filter paper under different pH values (4, 4.8, 5.5 and 6.5), temperatures (25, 28 and 34 °C) and incubation times (48, 72, 96 and 120 h).
Results: The optimum condition for cellulase production by T. harzianum is 120 hours of incubation at 25 °C and pH of 6.5.
Conclusion: T. harzianum can be used for the production of all three classes of cellulase. This fungus is suitable for the efficient production of cellulolytic enzymes and reducing the cost of consumables.
Keywords: Cellulose, Trichoderma harzianum, Hydrolytic enzymes, Optimization.
Full-Text [PDF 685 kb]   (997 Downloads)    
Type of Study: Original Paper |
Received: 2017/01/28 | Accepted: 2017/01/28 | Published: 2017/01/28 | ePublished: 2017/01/28

1. Chaston TB, Dixon JB. Factors associated with percent change in visceralversus subcutaneous abdominal fat during weight loss:findings from a systematic review. International Journal of Obesity. 2008; 32(4): 619-28. doi: 10.1038/sj.ijo.0803761. [DOI:10.1038/sj.ijo.0803761]
2. Akhmedov D, Berdeaux R. The effects of obesity on skeletal muscle regeneration. Front Physiol. 2013; 4: 371. doi: 10.3389/fphys.2013.00371. [DOI:10.3389/fphys.2013.00371]
3. World Health Organization. Obesity. Available from: p://www.who.int/topics/obesity/en. Accessed October 19, 2013.
4. Lopes AL, Fayh APT, Gde Souza Campos L, Teixeira BC, Kreismann RB, Ribeiro JL, et al. The effects of diet- and diet plus exercise-induced weight loss on basal metabolic rate and acylated ghrelin in grade 1 obese subjects. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy. 2013; 6: 469-475. [DOI:10.2147/DMSO.S53501]
5. Lavie CJ, Milani RV, Ventura HO. Obesity and Cardiovascular Disease:Risk Factor, Paradox, and Impact of Weight Loss. J Am Coll Cardiol. 2009; 53(21): 1925-32. doi: 10.1016/j.jacc .
6. Klempel MC, Kroeger CM, Varady KA. Alternate day fasting increases LDL particle size independently of dietary fat content in obese humans. Eur J Clin Nutr. 2013; 67(7): 783-5. doi: 10.1038/ejcn.2013.83. [DOI:10.1038/ejcn.2013.83]
7. Romero Moraleda B, Morencos E, Peinado AB, Bermejo L, Gómez Candela C, Benito PJ, et al. Can the exercise mode determine lipid profile improvements in obese patients? Nutr Hosp. 2013; 28(3): 607-617.
8. Ho SS, Dhaliwal SS, Hills AP, Pal S. The effect of 12 weeks of aerobic, resistance orcombination exercise training on cardiovascularrisk factors in the overweight and obese in arandomized trial. BMC Public Health. 2012; 12: 704. doi: 10.1186/1471-2458-12-704. [DOI:10.1186/1471-2458-12-704]
9. A Varady K, Bhutani S, C KlempelM , M Kroeger C. Comparison of effects of diet versus exerciseweight loss regimens on LDL and HDL particlesize in obese adults. Lipids in Health and Disease. 2011; 10: 1-5. doi: 10.1186/1476-511X-10-119. [DOI:10.1186/1476-511X-10-119]
10. Tzotzas T, Evangelou P, Kiortsis DN. Obesity, weight loss and conditional cardiovascular risk factors. obesity reviews. 2011; 12(5):e282-9. doi: 10.1111/j.1467-789X.2010.00807.x. [DOI:10.1111/j.1467-789X.2010.00807.x]
11. Aadahl M, von Huth Smith L, Pisinger C, Toft UN, Glümer C, Borch-Johnsen K, Jørgensen T. Five-year change in physical activity is associated with changes in cardiovasculardisease risk factors. Preventive Medicine. 2009; 48(4):326-31. doi: 10.1016/j.ypmed.2009.01.015. [DOI:10.1016/j.ypmed.2009.01.015]
12. Rush EC, Chandu V, Plank LD. Reduction of abdominal fat and chronic disease factors by lifestyle change inmigrant Asian Indians older than 50 years. Asia Pac J Clin Nutr. 2007; 16(4): 671-676.
13. Yoshida H, Ishikawa T, Suto M, Kurosawa H, Hirowatari Y, Ito K, Yanai H, Tada N, Suzuki M. Effects of supervised aerobic exercise training on serum adiponectin and parameters of lipid and glucose metabolism in subjects with moderate dyslipidemia. J AtherosclerThromb. 2010; 17(11): 1160-1166. [DOI:10.5551/jat.4358]
14. Morencos E, Romero B, Peinado AB, González-Gross M, Fernández C, Gómez-Candela C, Benito PJ. Effects of dietary restriction combined with different exercise programs or physical activity recommendations on blood lipids in overweight adults. Nutr Hosp. 2012; 27(6): 1916-27. doi: 10.3305/nh.2012.27.6.6057.
15. Tartibian B, Yaghoobnezhad F, Saboory E, kheradmand F. The Response of Serum Cortisol and Lipid Profile to a Moderately Intensive Aerobic Exercise in Non-Active Middle-Aged Men. Urmia Medical Journal. 2013; 24(6): 393-404.
16. Eatemady-Boroujeni A, Kargarfard M, Mojtahedi H, Rouzbehani R, Dastbarhagh H. Comparison of the Effects of 8-Weeks Aerobic Training and Resistance Training on Lipid Profile in Patients with Diabetes Type 2. J Isfahan Med Sch. 2014; 32(282): 524-33.[Persian]
17. TrussardiFayh AP, Lopes AL, da Silva AM, Reischak-Oliveira A, Friedman R. Effects of 5 % weight loss through diet or diet plus exercise on cardiovascular parameters of obese: a randomized clinical trial. Eur J Nutr. 2013; 52(5):1443-50. doi: 10.1007/s00394-012-0450-1. [DOI:10.1007/s00394-012-0450-1]

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