Volume 10, Issue 6 (Nov-Dec-2016 2016)                   mljgoums 2016, 10(6): 21-27 | Back to browse issues page


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Vahideh assadollahi V, Jalalvand M, Bagheri S, Esmaiel Lashkarian H. Effect of Mouse Liver Extract on in Vitro Differentiation of Amniotic Membrane Stem Cells into Hepatocyte-Like Cells. mljgoums. 2016; 10 (6) :21-27
URL: http://mlj.goums.ac.ir/article-1-916-en.html
1- Department of Biochemistry and Genetic, Faculty of Medicine
2- Department of Biotechnology, Faculty of Medicine , masumehjalalvand@gmail.com
Abstract:   (6826 Views)

ABSTRACT

          Background and Objective: Multipotent placental amniotic membrane mesenchymal stem cells (MSCs) are capable of differentiating into specialized tissues under different conditions. The aim of this study was to induce differentiation of placental amniotic membrane MSCs from NMRI mouse into hepatocytes using liver extract.

         Methods: Placental amniotic membrane MSCs from a 14-day pregnant female mouse was used in this study. The cells were incubated with trypsin solution, followed by pipetting. The resulting suspension was cultured in 12-well plates. After confirming their mesenchymal nature, differentiation of the aforementioned cells was induced via exposure to 6, 18, 30 and 60 μg/ml of liver extract. On the 16th day of treatment, immunocytochemical reaction for albumin and periodic acid-Schiff (PAS) test were performed for detection of hepatocyte-like cells.

          Results: Change was observed in the shape of differentiating cells from spindle-like shape to polygonal shape. The immunocytochemical reaction of the differentiated cells was positive. PAS staining also confirmed the accumulation of glycogen particles in the aforementioned cells. Concentration of 6 μg/ml liver extract was found as the effective dose for induction of differentiation.

           Conclusion: The findings of this study show that the placental amniotic membrane-derived MSCs of mouse can differentiate in vitro from spindle-like cells to polygonal hepatocyte-like cells with large nuclei and under the influence of the liver.

Keywords: Placental Amniotic Membrane Mesenchymal Stem Cells, Hepatocyte, In Vitro.

Full-Text [PDF 511 kb]   (775 Downloads)    
Type of Study: Original Paper |
Received: 2017/01/25 | Accepted: 2017/01/25 | Published: 2017/01/25 | ePublished: 2017/01/25

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