Abstract Bachground and objectives:Streptomyces is the most important genus in Actinomycetes family.The Streptomycetes are widely used in industry producing numerous chemical compounds including antibiotics, enzymes and anti- tumor agents. The aim of this study was to isolate soil-borne Streptomyces producing antimicrobial substances from soil of Golestan province of Iran and to survey anti-fungal metabolities produced by this organism. Material and Methods:In this study various soil samples were collected: ( forest areas of Naharkhoran in Gorgan and Kordkuy’s Derazno, Aghala’s deserts and agriculture lands of Aliabad ) were cultured on Actinomycet isolation agar and Starch casein agar were identified and purified by morphology and biochemistry tests. The activity of isolated Streptomyses against:Aspergillus flavus, Aspergillus, Candida albicans and Malasesia fur fur were studied by Agar Diffusion. Results:Of 120 samples, 24 are Streptomyces(20%) .The frequency of Streptomyces are reported in Aghala (10,41.6%),Derzno (8,33.3%) ,Nahar khoran(4,16.6%) and Aliabad(2,8.3%).Of 24 isolated Sterptomyses,two isolates have strong anti-fungal and six of them have moderate effect.We also see Streptomyses,isolated from desert area, have higher anti-fungal activity. Conclusion:It is recommended two isolated of Streptomyses be identified ana purified. Key words:Streptomyces , antifungal activities, antibiotic