YMDD and FLLAQ Mutations in Chronic Hepatitis B Patients

Rezanezhadi, M,; Tabarraei, A,; Zhand, S,; Moradi, A,; Nezamzade, R,; Vakili, MA,

Volume 8, Issue 5 (winter[PERSIAN] 2015) mljgoums 2015, 8(5): 7-13 | Back to browse issues page

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Rezanezhadi, M, Tabarraei, A, Zhand, S, Moradi, A, Nezamzade, R, Vakili, MA. YMDD and FLLAQ Mutations in Chronic Hepatitis B Patients. mljgoums 2015; 8 (5) :7-13
URL: http://mlj.goums.ac.ir/article-1-550-en.html

YMDD and FLLAQ Mutations in Chronic Hepatitis B Patients

Rezanezhadi, M¹

, Tabarraei, A

², Zhand, S³

, Moradi, A³

, Nezamzade, R¹

, Vakili, MA⁴

1- Islamic Azad University, Damghan Branch, Iran
2- Infectious Diseases Research Centre, Golestan University of Medical Sciences, Gorgan, Iran , aligant@yahoo.com
3- Golestan University of Medical Sciences, Gorgan, Iran
4- Infectious Diseases Research Centre, Golestan University of Medical Sciences, Gorgan, Iran

Abstract: (17608 Views)

Abstract Background and Objective: Lamivudine is the first orally available drug approved for treatment of chronic hepatitis B. Mutations at the YMDD and FLLAQ motifs in the domains of HBV polymerase gene contribute resistance to lamivudine. This study was aimed to determine the rate of YMDD and FLLAQ mutants in hepatitis B patients in Golestan Province, Iran. Material and methods: In this cross sectional study, 120 patients with chronic HBV infection were recruited. Of them, 55 were treated and 65 untreated with Lamivudine. HBV DNA extractions from plasma and polymerase chain reaction (PCR) were performed. For detection of Lamivudine mutants direct sequencing and alignment of products were applied using reference sequence from Gene Bank database. Results: the average age of patients was 36.31±10.07, which 35% of them were female and 65% were male. Mutations at the YMDD and FLLAQ motifs in the domains of HBV polymerase gene were detected in 12 of 55 patients (21.81%) treated with Lamivudine while no mutation was observed in in untreated patients. The YMDD and FLLAQ mutants were detected in 9.16% (11/120) and 0.83% (1/120) of chronic HBV patients, respectively. Conclusion: Usual HBV mutations, which play an important role in lamivudine resistance, detected in this study are similar to other studies. Key words: Hepatitis B Viruse, YMDD Mutation, Lamivudine, Iran.

Keywords: Hepatitis B Viruse, YMDD Mutation, Lamivudine, Iran.

Full-Text [PDF 680 kb] | Full Text (HTML) [English Abstract] (2552 Downloads)

Research Article: Original Paper |
Received: 2014/09/22 | Accepted: 2014/09/22 | Published: 2014/09/22 | ePublished: 2014/09/22