Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats

Kargar, M,; Ebrahimi, E,; Amini, J,; Najafi, A,; Kheirkhah, B,

Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015) mljgoums 2015, 8(4): 27-33 | Back to browse issues page

Mendeley

Zotero

RefWorks

Kargar, M, Ebrahimi, E, Amini, J, Najafi, A, Kheirkhah, B. Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats. mljgoums 2015; 8 (4) :27-33
URL: http://mlj.goums.ac.ir/article-1-533-en.html

Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats

Kargar, M

¹, Ebrahimi, E²

, Amini, J³

, Najafi, A⁴

, Kheirkhah, B⁵

1- Jahrom Branch, Islamic Azad University, Jahrom, Iran. , mkargar@jia.ac.ir
2- Islamic Azad University, Jahrom Branch, Iran
3- Islamic Azad University, Kerman Branch, Iran.
4- The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran
5- Islamic Azad University, Kerman Branch, Iran

Abstract: (18050 Views)

Abstract Background and Objective: Listeria monocytogenes is a bacterium transferred by foods and is the agent of many sporadic and epidemic diseases in humans. This study aimed to investigate the prevalence of L. monocytogenes and to determinine their antibiotic resistance profile in red meats. Material and Methods: this cross-sectional study was performed on 400 red meat samples obtained from industrial slaughterhouses placed in Kerman, Iran. First, the samples were enriched with Simultaneous Enrichment Broth (SEB), and then plated onto Palcam agar and Tryptic Soy Broth Yeast Extract Broth (TSAYE). After identification of the isolates based on biochemical tests and PCR, the isolates were checked for their antibiotic resistance profile using disk Diffusion Results: of 400 samples, 12 samples (3%) were contaminated with different species of Listeria. Using PCR, hly gene was recognized in eight samples (2%) of L. monocytogenes. Furthermore, there was a significant difference in isolation rate of lamb samples compared to cow ones. While all of the isolates were resistant to clindamycin, amikacin and chloramphenicol, they were sensitive to penicillin. Conclusion: in spite of low rate of infection in red meat samples in Kerman city, due to high risk of Listeria contamination in red meats, we recommend applying a routine screening to identify this bacterium in our county. Keywords: Listeria Monocytogenes, Hly Gene, Red Meat, Antibiotic, Kerman

Keywords: Listeria Monocytogenes, Hly Gene, Red Meat, Antibiotic, Kerman

Full-Text [PDF 709 kb] | Full Text (HTML) [English Abstract] (3003 Downloads)

Research Article: Original Paper |
Received: 2014/08/31 | Accepted: 2014/08/31 | Published: 2014/08/31 | ePublished: 2014/08/31