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Volume 8, Issue 1 (spring[PERSIAN] 2014)
mljgoums 2014, 8(1): 36-41 |
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Jamshidi A, Mousavi Ghahfarrokhi M, Gharaei A, Ebrahimzadeh A, Jaffari Modrek M, Ansari Moghadam A et al . Genotyping of C and F Regions of Plasmodium Falciparum EBA-175 in South-East of Iran. mljgoums 2014; 8 (1) :36-41
URL: http://mlj.goums.ac.ir/article-1-407-en.html
URL: http://mlj.goums.ac.ir/article-1-407-en.html
A Jamshidi 
1, M Mousavi Ghahfarrokhi , A Gharaei , A Ebrahimzadeh , M Jaffari Modrek , A Ansari Moghadam , S Mohammadi
1, M Mousavi Ghahfarrokhi , A Gharaei , A Ebrahimzadeh , M Jaffari Modrek , A Ansari Moghadam , S Mohammadi
1- , jamshidiali91@yahoo.com
Abstract: (23152 Views)
Abstract
Background and Objective: The Plasmodium falciparum EBA-175, via Sialic acid dependent glycophorin A, binds to red blood cells and thus plays a critical role in cell invasion. Some part of second allele in its gene encoding in FCR-3 (Section F) and CAMP (Section C) can be found. This study aimed to determine the prevalence of Plasmodium falciparum EBA-175KD alleles in southeastern Iran.
Material and Methods: In this cross-sectional study, using polymerase chain reaction Nest (Nested-PCR) with specific primers was used for the two parts of the EBA-175 gene to be proliferated. Ninety–four microscopic positive blood samples from individuals infected by Plasmodium falciparum were obtained from four different locations in southeastern Iran.
Results: Of 94 positive samples, 88 were antigen EBA-175KD. Genotype CAMP (714 bp) and FCR-3 (to 795 bp), respectively, in 31 (32.97 %) and 49 (52.12 %) were found. Eight samples have both FCR-3 and CAMP.
Conclusion: Both of EBA-175KD dimorphic genes were found. The frequency of FCR-3 allele was higher in the South East of Iran. Thus, this pattern can be considered in making Plasmodium falciparum vaccines for this area.
Key words: Plasmodium Falciparum Erythrocyte Binding Antigen-175 South-East of Iran
Research Article: Original Paper |
Received: 2014/01/12 | Accepted: 2014/01/12 | Published: 2014/01/12 | ePublished: 2014/01/12
Received: 2014/01/12 | Accepted: 2014/01/12 | Published: 2014/01/12 | ePublished: 2014/01/12
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.