Drug- Resistance- Associated Mutations and HIV Sub-Type Determination in Drug-Naïve and HIV-Positive Patients under Treatment with Antiretroviral Drugs

Naziri, H; Tabarraei, A; Ghaemi, A; Davarpanah, MA; Javid, N; Moradi, A

Volume 7, Issue 3 (Autumn 2013) mljgoums 2013, 7(3): 1-8 | Back to browse issues page

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Naziri H, Tabarraei A, Ghaemi A, Davarpanah M, Javid N, Moradi A. Drug- Resistance- Associated Mutations and HIV Sub-Type Determination in Drug-Naïve and HIV-Positive Patients under Treatment with Antiretroviral Drugs . mljgoums 2013; 7 (3) :1-8
URL: http://mlj.goums.ac.ir/article-1-299-en.html

Drug- Resistance- Associated Mutations and HIV Sub-Type Determination in Drug-Naïve and HIV-Positive Patients under Treatment with Antiretroviral Drugs

H Naziri¹

, A Tabarraei²

, A Ghaemi³

, MA Davarpanah⁴

, N Javid⁵

, A Moradi

⁶

1- MSc of Medical Virology, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences
2- Assistant Professor of Medical Virology Department of Microbiology, School of Medicine, Infectious Diseases Research Centre
3- Assistant Professor of Medical Virology, Department of Microbiology, School of Medicine
4- Infectious disease specialist, Shiraz HIV and AIDS Research Center
5- MSc of Microbiology, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences
6- Professor of Medical Virology, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, , abmoradi@yahoo.com

Abstract: (17552 Views)

Abstract Background and Objective: Resistance to antiretroviral agents is a significant concern in clinical management of HIV-infected individuals. Resistance is the result of mutations that develops in the viral protein targeted by antiretroviral agents. Material and Methods: In this cross-sectional study, the blood samples of 40 HIV-positive patients were collected. Twenty of them were drug-naïve and the rest were under treatment for at least one year by antiretroviral agents. Virus genome was extracted from patient's plasma with high-pure-viral-nucleic-acid kit. Then, by means of reverse-transcriptase and specific primers of protease genes were amplified and sequenced. Sequences of genes, drug- antiretroviral- resistant mutations and subtypes were determined using Stanford University’s HIV-drug-resistance databases. Results: Drug-naive patients show 15% resistance to nucleoside-reverse-transcriptase inhibitor (NRTI) and 20% resistance to non-nucleoside-reverse-transcriptase inhibitor (NNRTI). Anti-protease resistance is not observed in any patients. In under treatment patients, drug resistance to NNRTI (25%) is more than drug resistance to NRTI (20%) and the rate of drug resistance to protease inhibitor is 5%. Conclusion: Our findings show a high prevalence of drug-resistant mutations in Iranian-drug-naïve-HIV-infected patients. But in under treatment individuals, the rate of drug resistance is less than previous studies. Keywords: HIV Nucleoside Inhibitor Non-Nucleoside Inhibitor Protease Inhibitor