Isolation, Molecular Identification and Evaluation of Antifungal Effects of the Bacilli Isolated from Rhizospheric Soil of Gorgan Region against Tricophyton Mentagrophytes

Pordeli, HR; Hashemi Hazaveh, SJ; Jamshidian, M; Bayat, M

Volume 7, Issue 2 (summer[PERSIAN] 2013) mljgoums 2013, 7(2): 18-22 | Back to browse issues page

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Pordeli H, Hashemi Hazaveh S, Jamshidian M, Bayat M. Isolation, Molecular Identification and Evaluation of Antifungal Effects of the Bacilli Isolated from Rhizospheric Soil of Gorgan Region against Tricophyton Mentagrophytes. mljgoums 2013; 7 (2) :18-22
URL: http://mlj.goums.ac.ir/article-1-271-en.html

Isolation, Molecular Identification and Evaluation of Antifungal Effects of the Bacilli Isolated from Rhizospheric Soil of Gorgan Region against Tricophyton Mentagrophytes

HR Pordeli

¹, SJ Hashemi Hazaveh²

, M Jamshidian³

, M Bayat⁴

1- Assistant Professor of Mycology, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Gorgan Branch , h_pordeli@yahoo.com
2- Associated Professor of Mycology, Department of Mycology, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
3- Professor of Microbiology, Department of Microbiology, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
4- Associated Professor of Mycology, Department of Mycology, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran

Abstract: (39987 Views)

Abstract Background and objective: Soil bacteria, particularly Bacillus genus have the potential of producing a range of bioactive substances with antimicrobial and antifungal properties. They have the ability to produce hundreds of active and effective biologic compound against microorganisms. Therefore, it seems to be a proper candidate in the biocontrol of fungal pathogenesis. Material & Methods: In this study, soil samples were collected from different parts of Gorgan in order to isolate Bacillus and to determine their antifungal activity against T.mentagrophytes. The Isolates that had the highest antifungal effects were analyzed by PCR and 16s rRNA sequencing. Results: of 54 strains, 14 have antifungal activity. The Isolates, S4 and S12, identified as B.cereus and B.thuringiensis respectively show the highest antidermatofit effect. These isolates based on 16s rRNA sequence analysis show 97% homology with Bacillus cereusstrain KU4 and Bacillus thuringiensisstrain ucsc27. Conclusion: According to the results, it seems that the soil Bacilli have biocontrol potential against dermatophytic agents such as T.mentagrophytes. Keywords: Antifungal effects, Bacillus, Rhizospheric soil, T.mentagrophytes

Keywords: Antifungal effects, Bacillus, Rhizospheric soil, T.mentagrophytes

Full-Text [PDF 630 kb] [English Abstract] (3702 Downloads)

Research Article: Original Paper |
Received: 2013/05/1 | Accepted: 2013/10/5 | Published: 2013/10/5 | ePublished: 2013/10/5