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1- Department of Biology, Ra.C., Islamic Azad University, Rasht, Iran , zahranasirzade94@gmail.com
2- Department of Biology, Ra.C., Islamic Azad University, Rasht, Iran
2- Department of Biology, Ra.C., Islamic Azad University, Rasht, Iran
Abstract: (194 Views)
Abstract
Background and Objective: Currently, healthcare-associated infections (HAIs) are rising alarmingly due to antibiotic-resistant gram-negative bacteria, particularly Pseudomonas aeruginosa and Acinetobacter species. The study in Rasht focuses on identifying phenotypic and molecular metallo-β-lactamases (MBLs) in clinical isolates of Pseudomonas aeruginosa and Acinetobacter that display resistance to Imipenem.
Methods: In this study, 52 Acinetobacter and 25 Pseudomonas aeruginosa samples were collected from medical centers in Rasht. The clinical isolates were analyzed using various biochemical tests. The antibiotic resistance patterns of the isolates were determined by the disk diffusion method, following CLSI guidelines. Isolates producing metallo-β-lactamases (MBLs) were identified using the IPM+EDTA combined test method. Genomic DNA was subsequently extracted from the isolates using a commercial kit, and the presence of blaIMP and blaVIM genes was determined by PCR.
Results: According to the findings of this study, the highest percentage of resistance in Acinetobacter was observed with cefotaxime antibiotics at 94.23%, and in Pseudomonas aeruginosa with ceftazidime at 84%. Among the clinical isolates not susceptible to Imipenem, 18 (40%) were Acinetobacter and 2 (15.38%) were Pseudomonas aeruginosa, as determined by the combined disc method. The blaIMP and blaVIM genes were detected in 7 (13.46%) and 11 (21.15%) of the Acinetobacter samples, respectively, and in 8 (32%) and 5 (20%) of the Pseudomonas aeruginosa samples, respectively.
Conclusion: These results suggest that the blaIMP and blaVIM genes play important roles in the antibiotic resistance and potentially influence the virulence of Acinetobacter species and Pseudomonas aeruginosa in the region
Background and Objective: Currently, healthcare-associated infections (HAIs) are rising alarmingly due to antibiotic-resistant gram-negative bacteria, particularly Pseudomonas aeruginosa and Acinetobacter species. The study in Rasht focuses on identifying phenotypic and molecular metallo-β-lactamases (MBLs) in clinical isolates of Pseudomonas aeruginosa and Acinetobacter that display resistance to Imipenem.
Methods: In this study, 52 Acinetobacter and 25 Pseudomonas aeruginosa samples were collected from medical centers in Rasht. The clinical isolates were analyzed using various biochemical tests. The antibiotic resistance patterns of the isolates were determined by the disk diffusion method, following CLSI guidelines. Isolates producing metallo-β-lactamases (MBLs) were identified using the IPM+EDTA combined test method. Genomic DNA was subsequently extracted from the isolates using a commercial kit, and the presence of blaIMP and blaVIM genes was determined by PCR.
Results: According to the findings of this study, the highest percentage of resistance in Acinetobacter was observed with cefotaxime antibiotics at 94.23%, and in Pseudomonas aeruginosa with ceftazidime at 84%. Among the clinical isolates not susceptible to Imipenem, 18 (40%) were Acinetobacter and 2 (15.38%) were Pseudomonas aeruginosa, as determined by the combined disc method. The blaIMP and blaVIM genes were detected in 7 (13.46%) and 11 (21.15%) of the Acinetobacter samples, respectively, and in 8 (32%) and 5 (20%) of the Pseudomonas aeruginosa samples, respectively.
Conclusion: These results suggest that the blaIMP and blaVIM genes play important roles in the antibiotic resistance and potentially influence the virulence of Acinetobacter species and Pseudomonas aeruginosa in the region
Research Article: Research Article |
Subject:
Microbiology
Received: 2024/08/12 | Accepted: 2025/08/10
Received: 2024/08/12 | Accepted: 2025/08/10
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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