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Speciation of Candida isolated from clinical samples by Matrix Assisted Laser Desorption Ionization – Time of Flight Mass spectrometry (MALDI-TOF MS) Analysis.
Kavitha Prabhu1 , Rashmi Dsouza2 , Santhosha Devadiga3 , Beena Antony4 , Meena Dias5
1- MD Medical Microbiology, Assistant Professor in the department of Microbiology, Father Muller Medical College, Mangalore. India
2- JRF/CRC. Father Muller Research centre, Mangalore, India
3- Department of clinical virology, Father Muller Research centre, Mangalore, India.
4- Department of Microbiology, Father muller Research Centre, Mangalore, India. , beenafmmc@gmail.com
5- Department of Microbiology, Father Muller Medical College, Mangalore, India.
Abstract:   (158 Views)
Introduction
Candida species cause significant morbidity and mortality in vulnerable populations. Recent reports suggest a shift in the epidemiological trend to non albicans Candida infections from Candida albicans. This study was done to speciate candida from clinical samples using Matrix Assisted Laser Desorption Ionization – Time of Flight Mass spectrometry (MALDI-TOF MS) Analysis.
Materials and Methods
All routine clinical samples received in the bacteriology and mycology sections were processed using standard protocol. Candida species recovered from various clinical samples were speciated using MALDI-TOF MS from September 2022 to November 2023 and results were tabulated and analyzed statistically.
Results: A total of 342 Candida species were isolated during the study period. Median age of the patients presenting with candidiasis was 46.5 years with male predominance. Non albicans Candida species were predominant with C.tropicalis (30.40%), the most common species followed by C.parapsilosis(14.32%). Forty strains of C.auris isolates were found during this 15 months study period (11.69%).
Conclusion: The role of Candida species, including the non albicans Candida and newly emerging drug resistant candida strains should be dealt with caution. With its rapid and accurate results, MALDI-TOF MS can replace conventional and automated machines which are based on biochemical reactions for the speciation of Candida isolates.
 
Keywords: Candidiasis, non albicans Candida, MALDI-TOF.
Full-Text [PDF 478 kb]   (75 Downloads)    
Research Article: Original Paper | Subject: Mycology
Received: 2024/03/26 | Accepted: 2024/12/31
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