Thu, Sep 4, 2025
Azam Shareh1 
, Behnoush Khasheii2 , Tayebe Faraji1 , Masoud Khoshnia3 
, Shaghayegh Anvari4 , Ailar Jamalli5
, Behnoush Khasheii2 , Tayebe Faraji1 , Masoud Khoshnia3 , Shaghayegh Anvari4 , Ailar Jamalli5
1- Department of Microbiology, Faculty of Medical Sciences, Golestan University of Medical Sciences, Gorgan, Iran.
2- Department of Pathobiology, Faculty of Veterinary Sciences, Bu-Ali Sina University Hamedan, Iran.
3- Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran.
4- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
5- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran. ,jamali@goums.ac.ir
2- Department of Pathobiology, Faculty of Veterinary Sciences, Bu-Ali Sina University Hamedan, Iran.
3- Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran.
4- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
5- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran. ,
Abstract: (56 Views)
Background and objectives: Antibiotic resistance in Helicobacter pylori is a significant cause of failure in the treatment of this infection. This study aimed to evaluate the antibiotic resistance to metronidazole, clarithromycin and fluoroquinolone in Helicobacter pylori strains isolated from biopsy specimens of patients.
methods: This study was carried out in (2016 to 2017) on 80 biopsy specimens of the Golestan province. Resistance to metronidazole (rdxA), fluoroquinolones (gyrA) was determined by PCR. Mutations at the A2143G and A2142G loci in the 23srRNA gene associated with clarithromycin resistance in strains were performed by PCR-RFLP with BsaI and BbsI enzymes.
Results: In this study, 25% of Helicobacter pylori strains were resistant to clarithromycin. A mutation in the A2143G locus (65%) and the A2142G locus (35%) were detected in these strains. Resistance to fluoroquinolones (27.5%) was observed, among which mutations in the 91 amino acid position of aspartate (63.63%) and mutations in the 87 amino acid position of asparagine (36.36%) were observed in the samples. Resistance to metronidazole was not observed in any of the strains of this study, and concomitant resistance to clarithromycin and fluoroquinolones was observed in (13.75%) of Helicobacter pylori strains.
Conclusions: According to our study, the resistance of Helicobacter pylori to clarithromycin, is increasing, which is a factor in treatment failure. The mechanism of clarithromycin resistance is related to mutations in the A2143G and A2142G positions, and resistance to fluoroquinolones is by a mutation in the gyrA gene and more in the 91 amino acid position.
methods: This study was carried out in (2016 to 2017) on 80 biopsy specimens of the Golestan province. Resistance to metronidazole (rdxA), fluoroquinolones (gyrA) was determined by PCR. Mutations at the A2143G and A2142G loci in the 23srRNA gene associated with clarithromycin resistance in strains were performed by PCR-RFLP with BsaI and BbsI enzymes.
Results: In this study, 25% of Helicobacter pylori strains were resistant to clarithromycin. A mutation in the A2143G locus (65%) and the A2142G locus (35%) were detected in these strains. Resistance to fluoroquinolones (27.5%) was observed, among which mutations in the 91 amino acid position of aspartate (63.63%) and mutations in the 87 amino acid position of asparagine (36.36%) were observed in the samples. Resistance to metronidazole was not observed in any of the strains of this study, and concomitant resistance to clarithromycin and fluoroquinolones was observed in (13.75%) of Helicobacter pylori strains.
Conclusions: According to our study, the resistance of Helicobacter pylori to clarithromycin, is increasing, which is a factor in treatment failure. The mechanism of clarithromycin resistance is related to mutations in the A2143G and A2142G positions, and resistance to fluoroquinolones is by a mutation in the gyrA gene and more in the 91 amino acid position.
Research Article: Research Article |
Subject:
Microbiology
Received: 2023/03/25 | Accepted: 2023/07/9
Received: 2023/03/25 | Accepted: 2023/07/9
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