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mljgoums 2023, 17(1): 35-41 |
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Nasiri A R, Karamibonari A R. Protective Effects of Melissa officinalis L. Extract on Gentamicin-induced Renal Failure in Diabetic Rats. mljgoums 2023; 17 (1) :35-41
URL: http://mlj.goums.ac.ir/article-1-1372-en.html
URL: http://mlj.goums.ac.ir/article-1-1372-en.html
1- Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
2- Department of Pharmacology, Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran , pharmakarami@yahoo.com
2- Department of Pharmacology, Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran , pharmakarami@yahoo.com
Abstract: (1213 Views)
Background and objectives: Gentamicin is an aminoglycoside antibiotic used in the treatment of Gram-negative bacterial infections. One of the side effects of this antibiotic is nephrotoxicity. In this study, the protective effect of Melissa officinalis L. extract on diabetes- and gentamicin-induced nephrotoxicity was studied.
Methods: Forty male Wistar rats were randomly divided into four groups. The first group received distilled water, and the second group received M. officinalis L. extract (100 mg/kg) for 28 days. The third group received streptozocin (60 mg/kg) for 18 days, and then received gentamicin (80 mg/kg) on day 20 for 8 consecutive days. The fourth group received streptozocin, gentamicin, and M. officinalis L. extract for 28 days. Serum levels of blood urea nitrogen (BUN), creatinine, glucose, and amylases were measured. The right kidney was maintained in 10% formalin for hematoxylin and eosin staining, and oxidative stress markers in the left kidney were assessed.
Results: In the third group, serum BUN, creatinine, glucose, amylase, and malondialdehyde (MDA) increased, while glutathione peroxidase, superoxide dismutase, and catalase activities decreased significantly compared to the other groups (P<0.05). The extract of M. officinalis L. significantly inhibited the enhancement of serum creatinine, BUN, glucose, amylase, and MDA (P<0.05). Histological examinations showed that diabetes and gentamicin could lead to kidney damage by inducing necrosis and inflammation. Finally, the extract of M. officinalis L. could significantly reduce the adverse effects of both gentamicin and diabetes (P<0.05).
Conclusion: The extract of M. officinalis L. improves biochemical parameters and histological lesions in diabetic rats treated with gentamicin.
Methods: Forty male Wistar rats were randomly divided into four groups. The first group received distilled water, and the second group received M. officinalis L. extract (100 mg/kg) for 28 days. The third group received streptozocin (60 mg/kg) for 18 days, and then received gentamicin (80 mg/kg) on day 20 for 8 consecutive days. The fourth group received streptozocin, gentamicin, and M. officinalis L. extract for 28 days. Serum levels of blood urea nitrogen (BUN), creatinine, glucose, and amylases were measured. The right kidney was maintained in 10% formalin for hematoxylin and eosin staining, and oxidative stress markers in the left kidney were assessed.
Results: In the third group, serum BUN, creatinine, glucose, amylase, and malondialdehyde (MDA) increased, while glutathione peroxidase, superoxide dismutase, and catalase activities decreased significantly compared to the other groups (P<0.05). The extract of M. officinalis L. significantly inhibited the enhancement of serum creatinine, BUN, glucose, amylase, and MDA (P<0.05). Histological examinations showed that diabetes and gentamicin could lead to kidney damage by inducing necrosis and inflammation. Finally, the extract of M. officinalis L. could significantly reduce the adverse effects of both gentamicin and diabetes (P<0.05).
Conclusion: The extract of M. officinalis L. improves biochemical parameters and histological lesions in diabetic rats treated with gentamicin.
Keywords: Gentamicin [MeSH], Streptozocin [MeSH], Melissa [MeSH], Kidney disease [MeSH], Rats [MeSH]
Research Article: Research Article |
Subject:
Others
Received: 2021/03/10 | Accepted: 2021/05/22 | Published: 2023/01/20 | ePublished: 2023/01/20
Received: 2021/03/10 | Accepted: 2021/05/22 | Published: 2023/01/20 | ePublished: 2023/01/20
References
1. Mi N, Zhang XJ, Ding Y, Li GH, Wang WD, Xian HX, et al. Branched-chain amino acids attenuate early kidney injury in diabetic rats. Biochem Biophys Res Commun. 2015; 466(2): 240-6 [View at Publisher] [DOI:10.1016/j.bbrc.2015.09.017] [PubMed] [Google Scholar]
2. Wang JY, Yang JH, Xu J, Jia JY, Zhang XR, Yue XD, et al. Renal tubular damage may contribute more to acute hyperglycemia induced kidney injury in non-diabetic conscious rats. J Diabetes Complications. 2015; 29(5):621-8. [View at Publisher] [DOI:10.1016/j.jdiacomp.2015.04.014] [PubMed] [Google Scholar]
3. Al-Majed AA, Mostafa AM, Al-Rikabi AC, Al-Shabanah OA. Protective effects of oral arabic gum administration on gentamicin-induced nephrotoxicity in rats. Pharmacol Res. 2002 ;46(5):445-51. [View at Publisher] [DOI:10.1016/S1043661802001251] [PubMed] [Google Scholar]
4. Casqueiro J, Casqueiro J, Alves C. Infections in patients with diabetes mellitus: A review of pathogenesis. Indian J Endocrinol Metab. 2012 ;16 Suppl 1(Suppl1): S27-36. [DOI:10.4103/2230-8210.94253] [PubMed] [Google Scholar]
5. Martins EN, Pessano NT, Leal L, Roos DH, Folmer V, Puntel GO, et al. Protective effect of Melissa officinalis aqueous extract against Mn-induced oxidative stress in chronically exposed mice. Brain Res Bull. 2012; 87(1): 74-9. [View at Publisher] [DOI:10.1016/j.brainresbull.2011.10.003] [PubMed] [Google Scholar]
6. Ghanbari E, Nejati V, Azadbakht M. Protective effect of royal jelly against renal damage in streptozotocin induced diabetic rats. Iranian Journal of Toxicology. 2015; 28(9): 1259-1263. [Google Scholar]
7. Aebi H. Catalase in vitro. Methods in Enzymology. 1984;105: 121-126 . [View at Publisher] [DOI:10.1016/S0076-6879(84)05016-3] [PubMed] [Google Scholar]
8. Hur E, Garip A, Camyar A, Ilgun S, Ozisik M, Tuna S, et al. The effects of vitamin d on gentamicin-induced acute kidney injury in experimental rat model. Int J Endocrinol. 2013;2013:313528. [DOI:10.1155/2013/313528] [PubMed] [Google Scholar]
9. Tuttle KR, Bakris GL, Bilous RW, Chiang JL, de Boer IH, Goldstein-Fuchs J,et al. Diabetic kidney disease: a report from an ADA Consensus Conference. Am J Kidney Dis. 2014 ;64(4):510-33. [View at Publisher] [DOI:10.1053/j.ajkd.2014.08.001] [PubMed] [Google Scholar]
10. Erdem A, Gündoğan NU, Usubütün A, Kilinç K, Erdem SR, Kara A,et al. The protective effect of taurine against gentamicin-induced acute tubular necrosis in rats. Nephrol Dial Transplant. 2000 ;15(8):1175-82 [View at Publisher] [DOI:10.1093/ndt/15.8.1175] [PubMed] [Google Scholar]
11. Cohen L, Lapkin R, Kaloyanides GJ. Effect of gentamicin on renal function in the rat. J Pharmacol Exp Ther. 1975 ;193(1):264-73. [PubMed] [Google Scholar]
12. Murali B, Goyal R. Effect of chronic treatment with losartan on streptozotocin induced diabetic nephropathy. Clinical and Experimental Hypertension. 2001;23(7):513-20. [View at Publisher] [DOI:10.1081/CEH-100106822] [PubMed] [Google Scholar]
14. Zhang S, Xu H, Yu X, Wang Y, Sun F, Sui D. Simvastatin ameliorates low-dose streptozotocin-induced type 2 diabetic nephropathy in an experimental rat model. Int J Clin Exp Med. 2015 15;8(4):6388-96. [PubMed] [Google Scholar]
15. Yazar E, Elmas M, Altunok V, Sivrikaya A, Oztekin E, Birdane YO. Effects of aminoglycoside antibiotics on renal antioxidants, malondialdehyde levels, and some serum biochemical parameters. Canadian journal of veterinary research. 2003;67(3): 239-240. [PubMed] [Google Scholar]
16. Safaeian L, Sajjadi SE, Javanmard SH, Montazeri H, Samani F. Protective effect of Melissa officinalis extract against H2O2-induced oxidative stress in human vascular endothelial cells. Res Pharm Sci. 2016 ;11(5):383-389. [DOI:10.4103/1735-5362.192488] [PubMed] [Google Scholar]
17. Shin Y, Lee D, Ahn J, Lee M, Shin SS, Yoon M. The herbal extract ALS-L1023 from Melissa officinalis reduces weight gain, elevated glucose levels and β-cell loss in Otsuka Long-Evans Tokushima fatty rats. J Ethnopharmacol. 2021 10;264:113360. [View at Publisher] [DOI:10.1016/j.jep.2020.113360] [PubMed] [Google Scholar]
18. Ashkani-Esfahani S, Ebrahimi A, Bahmani-Jahromi M, Nadimi E, Arabzadeh H, Jalalpour MH, et al. The Effect of Melissa officinalis Extract on Streptozotocin-Induced Diabetes in Rats: A Stereological Study on Pancreatic Islets and Beta-cells. Journal of Advances in Medical and Biomedical Research. 2021;29(132):34-40. [DOI:10.30699/jambs.29.132.34] [Google Scholar]
19. Sief M, Khalil F, Abou-Arab A, Abou Donia M, El-Sherbiny A, Mohamed S. Ameliorative role of Melissa officinalis against hepatorenal toxicities of organophosphorus malathion in male rats. MedCrave Online Journal of Toxicology. 2015;1(3):00013. [DOI:10.15406/mojt.2015.01.00014] [Google Scholar]
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