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mljgoums 2022, 16(5): 1-2 |
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Kalavi K, Faghihi M A, Jorjani O, Tatari Z. Transcriptome Analysis May Be Beneficial for Identification of Specific Pathways in Host Cell-Leishmania major Interactions(letter to editor). mljgoums 2022; 16 (5) :1-2
URL: http://mlj.goums.ac.ir/article-1-1337-en.html
URL: http://mlj.goums.ac.ir/article-1-1337-en.html
1- Laboratory Science Research Center, Faculty of Paramedicine, Golestan University of Medical Sciences, Gorgan, Iran , kalavi@goums.ac.ir
2- Department of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, Iran, and Department of Psychiatry and Behavioral Sciences, Miami, USA
3- Laboratory Science Research Center, Faculty of Paramedicine, Golestan University of Medical Sciences, Gorgan, Iran
4- DarouPakhsh Distribution Vet. Company, Iran
2- Department of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, Iran, and Department of Psychiatry and Behavioral Sciences, Miami, USA
3- Laboratory Science Research Center, Faculty of Paramedicine, Golestan University of Medical Sciences, Gorgan, Iran
4- DarouPakhsh Distribution Vet. Company, Iran
Abstract: (1792 Views)
Dear Editor,
There are several reports about the modulatory effects of intracellular pathogens, such as Leishmania spp., to help survival and replication inside host cells. They alter host cells' defense and offence mechanisms, and in case of leishmaniasis, the pathogen creates a hostile environment inside macrophages. We performed an RNA sequencing analysis of transcriptome changes in Leishmania major-infected human macrophages at four hour post infection. In this study, we investigated gene expression pattern of the infected macrophages against microbead (4.16 µm) polystyrene particles phagocytized and non-polarized macrophages as controls. Monocytes with high purity were isolated from healthy donors by magnetic-activated cell sorting and then differentiated into macrophages after 6-9 days of incubation at 37 ˚C.
In this study, we focused on some key interaction events between host cell and the pathogen; so some feature steps resulting from dictation strategies of the pathogen are presented below.
There are several reports about the modulatory effects of intracellular pathogens, such as Leishmania spp., to help survival and replication inside host cells. They alter host cells' defense and offence mechanisms, and in case of leishmaniasis, the pathogen creates a hostile environment inside macrophages. We performed an RNA sequencing analysis of transcriptome changes in Leishmania major-infected human macrophages at four hour post infection. In this study, we investigated gene expression pattern of the infected macrophages against microbead (4.16 µm) polystyrene particles phagocytized and non-polarized macrophages as controls. Monocytes with high purity were isolated from healthy donors by magnetic-activated cell sorting and then differentiated into macrophages after 6-9 days of incubation at 37 ˚C.
In this study, we focused on some key interaction events between host cell and the pathogen; so some feature steps resulting from dictation strategies of the pathogen are presented below.
Research Article: Letter to Editor |
Subject:
Human Genetics
Received: 2020/10/5 | Accepted: 2020/12/15 | Published: 2022/07/16 | ePublished: 2022/07/16
Received: 2020/10/5 | Accepted: 2020/12/15 | Published: 2022/07/16 | ePublished: 2022/07/16
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.