Volume 15, Issue 1 (Jan-Feb 2021)                   mljgoums 2021, 15(1): 26-32 | Back to browse issues page


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Hanif H, Elikaei A, Vazini H, Mohammadi A. Anticancer and Antibacterial Effect of Eucalyptus Camaldulensis, in Vitro. mljgoums. 2021; 15 (1) :26-32
URL: http://mlj.goums.ac.ir/article-1-1307-en.html
1- Department of Microbiology, Faculty of Biological Scinces, Alzahra University, Vanak St., Tehran, Iran
2- Department of Microbiology, Faculty of Biological Scinces, Alzahra University, Vanak St., Tehran, Iran , a.elikaei@alzahra.ac.ir
3- Nursing Department, Basic Sciences Faculty, Hamedan Branch, Islamic Azad University, Tehran, Iran.
Abstract:   (718 Views)
Background and objectives: The spread of infectious diseases and malignant diseases has been increasing in the recent years. The use of chemical drugs, in addition to the development of drug resistance, also cause serious side effects. We conducted the present study to examine the antibacterial, antiviral, and anti-cancer effects of E. camaldulensis as a herbal remedy.
Methods: We extracted E. camaldulensis using a hydroalcoholic solution. The antiviral effect of the plant was investigated at the time of the Herpes simplex virus entry and once the virus entered the cell. Moreover, we evaluated MIC and MBC of E. camaldulensis on Klebsiella pneumonia, Staphylococcus aureus, Streptococcus pyrogens, Streptococcus agalactiae, Acinetobacter baumannii, and Corynebacterium glutamicum. For the evaluation of cell cytotoxicity, HFF-2 (NCBI: C163) and A549 )ATCC: CCL81) cell lines were utilized.
Results: The results of the cytotoxicity test indicated that both cell lines were sensitive to the hydroalcoholic extracts of E. camaldulensis. The MIC for A. baumannii, K. pneumonia, and C. glutamicum was 6.25 µg/ml, and the MIC for S. aureus, S. pyogenes, and S. agalactiae was 12.5 µg/ml. MBC was evaluated as 25 µg/ml for S. aureus, S. pyogenes, and S. Agalactiae. It was 12.5 µg/ml for A. baumannii, K. pneumonia, and S. Agalactiae. IC50 value on entering the virus into the cell was 40 µg/ml, and following the absorption of the virus, the IC50 value was 80 µg/ml.
Conclusion: The results of this study demonstrated that E. camaldulensis is of antibacterial, antiviral, and anti-cancer potentials and could be used as a candidate for the preparation of a new drug.
 
Full-Text [PDF 752 kb]   (176 Downloads)    
Research Article: Original Paper | Subject: Microbiology
Received: 2020/07/25 | Accepted: 2020/09/19 | Published: 2021/01/1 | ePublished: 2021/01/1

References
1. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA: a cancer journal for clinicians. 2011; 61(2): 69-90. [DOI:10.3322/caac.20107] [Google Scholar]
2. Stewart BW, Kleihues P. World cancer report. International Agency for research on Cancer. 2003; 1(1). [View at Publisher]
3. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA: a cancer journal for clinicians. 2016;66(1):7-30. [DOI:10.3322/caac.21332] [PubMed] [Google Scholar]
4. Collins LG, Haines C, Perkel R, Enck RE. Lung cancer: diagnosis and management. Am Fam Physician. 2007; 75(1): 56-63. [PubMed] [Google Scholar]
5. Esghaei M, Ghaffari H, Esboei BR, Tapeh ZE, Salim FB, Motevalian M. Evaluation of anticancer activity of Camellia sinensis in the Caco-2 colorectal cancer cell line. Asian Pacific journal of cancer prevention: APJCP. 2018;19(6):1697. [DOI] [PubMed] [Google Scholar]
6. Murray PR, Rosenthal KS, Pfaller MA. Medical microbiology: Elsevier Health Sciences; 2015. [View at Publisher] [Google Scholar]
7. Drancourt M, Michel-Lepage A, Boyer S, Raoult D. The point-of-care laboratory in clinical microbiology. Clinical microbiology reviews. 2016;29(3):429-47. [DOI:10.1128/CMR.00090-15] [PubMed] [Google Scholar]
8. Su C, Zhan G, Zheng C. Evasion of host antiviral innate immunity by HSV-1, an update. Virology journal. 2016; 13(1): 38. [DOI:10.1186/s12985-016-0495-5] [PubMed] [Google Scholar]
9. Dimmock NJ, Easton AJ, Leppard KN. Introduction to modern virology: John Wiley & Sons; 2016. [PubMed] [Google Scholar]
10. Miller KD, Siegel RL, Lin CC, Mariotto AB, Kramer JL, Rowland JH, et al. Cancer treatment and survivorship statistics, 2016. CA: a cancer journal for clinicians. 2016; 66(4): 271-89. [DOI:10.3322/caac.21349] [PubMed] [Google Scholar]
11. Ginovyan M. Effect of heat treatment on antimicrobial activity of crude extracts of some Armenian herbs. Chemistry and Biology. 2017; 51(2): 113-7. [Google Scholar]
12. Luís Â, Duarte A, Gominho J, Domingues F, Duarte AP. Chemical composition, antioxidant, antibacterial and anti-quorum sensing activities of Eucalyptus globulus and Eucalyptus radiata essential oils. Industrial Crops and Products. 2016; 79: 274-82. [DOI:10.1016/j.indcrop.2015.10.055] [Google Scholar]
13. Rodrigues TO, Rousset PLA. Effects of torrefaction on energy properties of Eucalyptus grandis wood. Cerne. 2015; 15(4): 446-52. [Google Scholar]
14. Cook RL, Binkley D, Stape JL. Eucalyptus plantation effects on soil carbon after 20 years and three rotations in Brazil. Forest Ecology and Management. 2016; 359: 92-8. [DOI:10.1016/j.foreco.2015.09.035] [Google Scholar]
15. Chabra A, Rahimi-Esboei B, Habibi E, Monadi T, Azadbakht M, Elmi T, et al. Effects of some natural products from fungal and herbal sources on Giardia lamblia in vivo. Parasitology. 2019; 146(9): 1188-98. [DOI:10.1017/S0031182019000325] [PubMed] [Google Scholar]
16. Elmi T, Esboei BR, Sadeghi F, Zamani Z, Didehdar M, Fakhar M, et al. In Vitro Antiprotozoal Effects of Nano-chitosan on Plasmodium falciparum, Giardia lamblia and Trichomonas vaginalis. Acta Parasitologica. 2020: 1-14. [DOI:10.1007/s11686-020-00255-6] [PubMed] [Google Scholar]
17. Pourhajibagher M, Rahimi-Esboei B, Hodjat M, Bahador A. Sonodynamic excitation of nanomicelle curcumin for eradication of Streptococcus mutans under sonodynamic antimicrobial chemotherapy: Enhanced anti-caries activity of nanomicelle curcumin. Photodiagnosis and Photodynamic Therapy. 2020:101780. [DOI:10.1016/j.pdpdt.2020.101780] [PubMed] [Google Scholar]
18. Rahimi-Esboei B, Ebrahimzadeh M, Fathi H, Rezaei Anzahaei F. Scolicidal effect of Allium sativum flowers on hydatid cyst protoscolices. Eur Rev Med Pharmacol Sci. 2016; 20(1): 129-32.
19. Golami S, Rahimi-Esboei B, Mousavi P, Marhaba Z, Youssefi MR, Rahimi MT. Survey on efficacy of chloroformic extract of Artemisia annua against Giardia lamblia trophozoite and cyst in vitro. Journal of parasitic diseases. 2016; 40(1): 88-92. [DOI:10.1007/s12639-014-0453-3] [PubMed] [Google Scholar]
20. Chabra A, Rahimi-Esboei B, Habibi E, Monadi T, Azadbakht M, Elmi T, et al. Effects of some natural products from fungal and herbal sources on Giardia lamblia in vivo. Parasitology. 2019; 1-11. [DOI:10.1017/S0031182019000325] [PubMed] [Google Scholar]
21. Soković M, Glamočlija J, Marin PD, Brkić D, van Griensven LJ. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules. 2010; 15(11): 7532-46. [DOI:10.3390/molecules15117532] [PubMed] [Google Scholar]
22. Lowe H, Toyang NJ. Therapeutic antiviral agents containing cannabis cannabinoid derivatives. Google Patents; 2018. [Google Scholar]
23. Vuong QV, Hirun S, Chuen TL, Goldsmith CD, Munro B, Bowyer MC, et al. Physicochemical, antioxidant and anti-cancer activity of a Eucalyptus robusta (Sm.) leaf aqueous extract. Industrial Crops and Products. 2015; 64: 167-74. [DOI:10.1016/j.indcrop.2014.10.061] [Google Scholar]
24. Bhagat M, Sharma V, Saxena AK. Anti-proliferative effect of leaf extracts of Eucalyptus citriodora against human cancer cells in vitro and in vivo. 2012. [PubMed] [Google Scholar]
25. Döll-Boscardin PM, Sartoratto A, Maia S, de Noronha BHL, Padilha de Paula J, Nakashima T, et al. In vitro cytotoxic potential of essential oils of Eucalyptus benthamii and its related terpenes on tumor cell lines. Evidence-Based Complementary and Alternative Medicine. 2012; 2012. [DOI:10.1155/2012/342652] [PubMed] [Google Scholar]
26. D Esmaeili AMM, and A Tohidpour. Anti-Helicobacter Pylori Activities of Shoya Powder and Essential Oils of Thymus Vulgaris and Eucalyptus Globulus. Open Microbiology Journal. 2012; 6(11): 65-9. [DOI:10.2174/1874285801206010065] [PubMed] [Google Scholar]
27. Tameshkel S. The antimicrobial effect of methanol extracts of Eucalyptus, Satureia hortensis and Heracleum glabrescens on Giardia cysts. Med Lab J. 2012; 6(2): 21-6. [Google Scholar]
28. Elaissi A, Rouis Z, Salem NAB, Mabrouk S, ben Salem Y, Salah KBH, et al. Chemical composition of 8 eucalyptus species' essential oils and the evaluation of their antibacterial, antifungal and antiviral activities. BMC complementary and alternative medicine. 2012; 12(1): 81. [DOI:10.1186/1472-6882-12-81] [PubMed] [Google Scholar]
29. Ashour HM. Antibacterial, antifungal, and anticancer activities of volatile oils and extracts from stems, leaves, and flowers of Eucalyptus sideroxylon and Eucalyptus torquata. Cancer biology & therapy. 2008; 7(3): 399-403. [DOI:10.4161/cbt.7.3.5367] [PubMed] [Google Scholar]
30. Ghalem BR, Mohamed B. Antibacterial activity of leaf essential oils of Eucalyptus globulus and Eucalyptus camaldulensis. African journal of Pharmacy and pharmacology. 2008; 2(10): 211-5. [Google Scholar]
31. Elaissi A, Salah KH, Mabrouk S, Larbi KM, Chemli R, Harzallah-Skhiri F. Antibacterial activity and chemical composition of 20 Eucalyptus species' essential oils. Food Chemistry. 2011; 129(4): 1427-34. [DOI:10.1016/j.foodchem.2011.05.100] [Google Scholar]
32. El-Baz FK, Mahmoud K, El-Senousy WM, Darwesh O, ElGohary A. Antiviral-antimicrobial and schistosomicidal activities of Eucalyptus camaldulensis essential oils. Int J Pharm Sci Rev Res. 2015; 31(1): 262-8. [Google Scholar]
33. Cermelli C, Fabio A, Fabio G, Quaglio P. Effect of eucalyptus essential oil on respiratory bacteria and viruses. Current microbiology. 2008; 56(1): 89-92. [DOI:10.1007/s00284-007-9045-0] [PubMed] [Google Scholar]
34. Inouye S, Takizawa T, Yamaguchi H. Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. Journal of antimicrobial chemotherapy. 2001; 47(5): 565-73. [DOI:10.1093/jac/47.5.565] [PubMed] [Google Scholar]
35. Singh R, Chandra R, Bose M, Luthra PM. Antibacterial activity of Curcuma longa rhizome extract on pathogenic bacteria. Current Science. 2002: 737-40. [PubMed] [Google Scholar]

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