Volume 14, Issue 6 (Nov-Dec 2020)                   mljgoums 2020, 14(6): 34-40 | Back to browse issues page

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nasiraey M, Jafari chashmi A, Hashemvarzi S A. Effect of Adipose Tissue-Derived Stem Cell Transplantation and Six Weeks of Aerobic Exercise on FNDC5 and Irisin Levels in Streptozotocin-Induced Diabetic Rats. mljgoums. 2020; 14 (6) :34-40
URL: http://mlj.goums.ac.ir/article-1-1301-en.html
1- Department of Sport Physiology , mehdi.nasiraey@gmail.com
2- Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran
Abstract:   (381 Views)
Background and objectives: It has been suggested that irisin and fibronectin type III domain-containing protein 5 (FNDC5) can increase energy expenditure, promote weight loss and improve insulin resistance in diabetic patients by affecting white and brown adipose tissues. In this study, we investigate effect of adipose tissue-derived stem cell transplantation and six weeks of aerobic exercise on FNDC5 and irisin levels in streptozotocin-induced diabetic rats.
Methods: Forty-eight rats (weighing 220-240 g and aged nine weeks) were divided into six groups of control, sham, diabetes control, diabetes + exercise, diabetes + stem cell and diabetes+ exercise + stem cell. The exercise group ran on running wheel at intensity of 60-70% VO2max, five days a week for six weeks. Next, 1.56 × 106 stem cells extracted from human adipose tissue were injected into the tail vein of streptozotocin-induced diabetic rats. Finally, FNDC5 and irisin plasma levels of the mice were measured using enzyme-linked immunosorbent assay kits.  
Results: FNDC5 and irisin levels reduced significantly in the diabetes control group (P=0.0001). FNDC5 levels in the diabetes + exercise + stem cell and the exercise group increased significantly compared to the diabetes control group (P=0.0001). The irisin level in the diabetes + stem cell + exercise group, exercise group and stem cell group increased significantly compared to the diabetes control group (P=0.0001).  
Conclusion: The results indicate that aerobic exercise program and stem cell therapy alone and combined can significantly increase plasma irisin levels. Given the favorable effects of adipose tissue-derived stem cell injection and aerobic exercise on FNDC5 and irisin levels, this strategy could be further evaluated in coping with the adverse effects of diabetes on metabolism and aging
Full-Text [PDF 818 kb]   (84 Downloads)    
Research Article: Original Paper | Subject: Sport Physiology
Received: 2020/06/7 | Accepted: 2020/07/18 | Published: 2020/10/29 | ePublished: 2020/10/29

1. 1 - Havilah P, Pandit Vinodh B, Durga Prasad K .Adenosine Deaminase Activity in Type-2 Diabetes Mellitus - An Independent Marker of Glycemic Status and Stimulator of Lipid Peroxidation. Int J Chem and Life Sciences. 2013; 02(06): 1175-1178. doi: 10.1186/s12902-018-0284-9. [DOI:10.1186/s12902-018-0284-9] [Google Scholar]
2. 2 - Glovaci D, Fan W , Wong ND. Epidemiology in diabetes mellitus and cardiovascular disease. Curr Cardiol Rep. 2019; 21(4): 21. doi: 10.1007/s11886-019-1107-y. [DOI:10.1007/s11886-019-1107-y] [PubMed] [Google Scholar]
3. 3 - Meier JJ, Bhushan A, Butler AE, Rizza RA, Butler PC. Sustained beta cell apoptosis in patients with longstanding type 1 diabetes: indirect evidence for islet regeneration? Diabetologia. 2005 Nov;48(11): 2221-8. doi: 10.1007/s00125-005-1949-2. [DOI:10.1007/s00125-005-1949-2] [PubMed] [Google Scholar]
4. 4 - Butler AE, Janson J, Bonner-Weir S, Ritzel R, Rizza RA, Butler PC. Beta-cell deficit and increased beta-cell apoptosis in humans with type 2 diabetes. Diabetologia. 2005; 48(11): 2221-8.doi: 10.1007/s00125-005-1949-2. [DOI:10.1007/s00125-005-1949-2] [PubMed] [Google Scholar]
5. 5 - Roriz-Filho JS, Sá-Roriz TM, Rosset I, Camozzato AL, Santos AC, Chaves MLF, et al. (Pre)diabetes, brain aging, and cognition. Biochimica et Biophysica Acta. 2009; 1792: 432-443. [DOI:10.1016/j.bbadis.2008.12.003] [PubMed] [Google Scholar]
6. 6 - Jahangard A, Hamedinia M, Hosseini Kakhk A, Jafari A, Salehzadeh K. The effects of short-term supplementation of garlic extract on oxidative stress indicators in times of resting and exhaustive exercising on male football players. Iranian Journal Of Endocrinology And Metabolism (Ijem). 2013; 15 (1): 78-85. [Google Scholar]
7. 7 - Ebrahim Khosrow, Sajjad Ahmadizad, Reza Ghanimati, Abdullah Bagheri, Sirous Sheikhi, Mehdi Ghanimati. The effect of endurance training and garlic consumption on maximum oxygen consumption (VO2max) of inactive men. 2012, Applied Research in Management and Biological Sciences in Sports, No. 2, pp. 18-11.
8. 8-Johari Habibollah, Zahra Zamani, Mokhtar Mokhtari, Vahid Hemayatkhah, Houshang Jamali, Mohammad Yazdani. Evaluation of the effect of garlic extract on eliminating lead poisoning in all infants of rats. 2014, Journal of Fasa University of Medical Sciences, 4th year, No. 1, pp. 98-90.
9. 9- Shirzad H, Taji F, Pilehvarian AA, Hosseini SM, Rafieian Kopaei M. The preventive and therapeutic effects of fresh garlic extract and aerial parts on the growth of fibrosarcoma cells in mice. Journal of Shahid Sadoughi University of Medical Sciences and Health Services. 2012; 20(83): 133-141. [Google Scholar]
10. 10 - Finkelstein J, Joshi A, Hise MK. Association of physical activity and renal function in subjects with and without metabolic syndrome: a review of the Third National Health and Nutrition Examination Survey (NHANES III). Am J Kidney Dis. 2006; 48(3): 372-82. doi: 10.1053/j.ajkd.2006.05.013. [DOI:10.1053/j.ajkd.2006.05.013] [PubMed] [Google Scholar]
11. 11 - Johansson K, Neovius M, Hemmingsson E. Effects of anti-obesity drugs, diet, and exercise on weight-loss maintenance after a very-low-calorie diet or low-calorie diet: a systematic review and meta-analysis of randomized controlled trials. Am J Clin Nutr. 2014; 99(1): 14-23. doi: 10.3945/ajcn.113.070052 [DOI:10.3945/ajcn.113.070052] [PubMed] [Google Scholar]
12. 12 - Ormsbee MJ, Thyfault JP, Johnson EA, Kraus RM, Choi MD, Hickner RC. Fat metabolism and acute resistance exercise in trained men. J Appl Physiol (1985). 2007; 102(5): 1767-72. doi: 10.1152/japplphysiol.00704.2006. [DOI:10.1152/japplphysiol.00704.2006] [PubMed] [Google Scholar]
13. 13 - José María Moreno-Navarrete, Francisco Ortega, Marta Serrano, Ester Guerra, Gerard Pardo, Francisco Tinahones, et al. Irisin is expressed and produced by human muscle and adipose tissue in association with obesity and insulin resistance. J Clin Endocrinol Metab. 2013; 98(4): E769-78. doi: 10.1210/jc.2012-2749. [DOI:10.1210/jc.2012-2749] [PubMed] [Google Scholar]
14. 14 - Sanchis-Gomar F, Lippi G, Mayero S, Perez-Quilis C, García-Giménez JL. Irisin: a new potential hormonal target for the treatment of obesity and type 2 diabetes. J Diabetes. 2012; 4(3): 196.doi: 10.1111/j.1753-0407.2012.00194.x. [DOI:10.1111/j.1753-0407.2012.00194.x] [PubMed] [Google Scholar]
15. 15 - Polyzos SA, Kountouras J, Shields K, Mantzoros CS. Irisin: a renaissance in metabolism? Metabolism. 2013; 62(8): 1037-44.doi: 10.1016/j.metabol.2013.04.008. [DOI:10.1016/j.metabol.2013.04.008] [PubMed] [Google Scholar]
16. 16 - Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis.Nature. 2012; 481(7382): 463-8.doi: 10.1038/nature10777. [DOI:10.1038/nature10777] [PubMed] [Google Scholar]
17. 17 - Little JP, Safdar A, Wilkin GP, Tarnopolsky MA, Gibala MJ. A practical model of low-volume high-intensity interval training induces mitochondrial biogenesis in human skeletal muscle: potential mechanisms. The Journal of Physiology. 2010; 588(Pt 6): 1011-1022. [DOI:10.1113/jphysiol.2009.181743] [PubMed] [Google Scholar]
18. 18 - Handschin C, Spiegelman BM. The role of exercise and PGC-1a in inflammation and chronic disease. Nature. 2008; 454(7203): 463-469. [DOI:10.1038/nature07206] [Google Scholar]
19. 19 - Hamidi Perchikolaei. S. O, Falah Mohamadi. Z, Hajizadeh Moghadam. A. The effect of treadmill running with consumption of vitamin D3 on NGF levels in Parkinsonian rat's striatum. Sport Physiology. 2016; 8 (29): 91-102.
20. 20 - Tang Z, Yuan L, Gu C, Liu Y, Zhu L. Effect of exercise on the expression of adiponectin mRNA and GLUT4 mRNA in type 2 diabetic rats. J Huazhong Univ Sci Technolog Med Sci 2005; 25(2): 191-193. [DOI:10.1007/BF02873574] [PubMed] [Google Scholar]
21. 21 - De Salles Bf, Simão R, Fleck SJ, Dias I, Kraemer-Aguiar LG, Bouskela E. Effects of resistance training on cytokines. Int J Sports Med. 2010; 31(7): 441-50. [DOI:10.1055/s-0030-1251994] [PubMed] [Google Scholar]
22. 22 - Sheu WH, Chang TM, Lee WJ, Ou HC, Wu CM, Tseng LN, et al. Effect of weight loss on proinflammatory state of mononuclear cells in obese women. Obesity (Silver Spring). 2008; 16(5): 1033-8. [DOI:10.1038/oby.2008.37] [PubMed] [Google Scholar]
23. 23 - Boulé NG, Haddad E, Kenny GP, Wells GA, Sigal RJ. Effects of exercise on glycemic control and body mass in type 2 diabetes mellitus: a meta-analysis of controlled clinical trials. JAMA 2001; 286(10): 1218-27. [DOI:10.1001/jama.286.10.1218] [PubMed] [Google Scholar]
24. 24 - Tokmakidis SP, Zois CE, Volaklis KA, Kotsa K, Touvra AM. The effects of a combined strength and aerobic exercise program on glucose control and insulin action in women with type 2 diabetes. Eur J Appl Physiol. 2004; 92(4-5): 437-42. [DOI:10.1007/s00421-004-1174-6] [PubMed] [Google Scholar]
25. 25 - Eizadi Mojtaba, Laleh Behboudi. Effect of Acute and Chronic Exercise on Beta-Cell Function in Diabetic Patients. Knowledge & Health. 2012; 6(4): 15-19. [Google Scholar]
26. 26 - Huh JY, Mougios V, Kabasakalis A, Fatouros I, Siopi A, Douroudos, II, et al. Exercise-induced irisin secretion is independent of age or fitness level and increased irisin may directly modulate muscle metabolism through AMPK activation. J Clin Endocrinal Metab. 2014; 99(11): E2154-61. [DOI:10.1210/jc.2014-1437] [PubMed] [Google Scholar]
27. 27 - Tofighi A, Mohammadi Topuzabadi B. The effect of eight weeks high intensity interval training on plasma irisin and subcutaneous adipose tissue UCP-1in male obese rat. Journal of Sport in Biomotor Sciences. 2016; 8(16): 62-69. [Google Scholar]
28. 28 - Pourheydar Bagher, Maryam Shahi, Gholam Hossein Farjah. Evaluation of Apoptosis In Hippocampal Cells of Rat Following Intravenous Injection of Bone Marrow Stromal Cells In Ischemia-Reperfusion Model. The Journal of Urmia University of Medical Sciences. 2014; 25(7): 597 ISSN: 1027-3727. [Google Scholar]
29. 29 - Guo F, Lv S, Lou Y, Tu W, Liao W, Wang Y, et al. Bone marrow stromal cells enhance the angiogenesis in ischemic cortex after stroke: involvement of notch signalling. Cell Biol Int. 2012; 36(11): 997-1004. doi: 10.1042/CBI20110596. [DOI:10.1042/CBI20110596] [PubMed] [Google Scholar]
30. 30 - Moradi A, Mohammadi S, Hamidi Alamdari D. Effect of adipose tissue-derived stem cells on the control of the blood glucose level in diabetic rats. J Shahid Sadoughi Univ Med Sci. 2015; 23(8): 717-26. [Google Scholar]
31. 31 - Lock LT, Tzanakakis ES. Stem/Progenitor cell sources of insulin-producing cells for the treatment of diabetes. Tissue engineering. 2007; 13(7): 1399-412. [DOI:10.1089/ten.2007.0047] [PubMed] [Google Scholar]
32. 32 - Krishna KA, Rao GV, Rao KS. Stem cell-based therapy for the treatment of Type 1 diabetes mellitus. Regenerative med. 2007; 2(2): 171-77. [DOI:10.2217/17460751.2.2.171] [PubMed] [Google Scholar]
33. 33 - Taha MF, Hedayati V. Isolation, identification and multipotential differentiation of mouse adipose tissue derived stem cells. Tissue & cell. 2010; 42(4): 211-16. [DOI:10.1016/j.tice.2010.04.003] [PubMed] [Google Scholar]
34. 34 - Chae CH, Jung SL, An SH, Park BY, Wang SW, Cho IH, et al. Treadmill exercise improves cognitive function and facilitates nerve growth factor signaling by activating mitogen-activated protein kinase/extracellular signal regulated kinase1/2 in the streptozotocin-induced diabetic rat hippocampus. Neuroscience. 2009; 164: 1665-1673. [DOI:10.1016/j.neuroscience.2009.09.075] [PubMed] [Google Scholar]
35. 35 - Arturo Roca-Rivada , Cecilia Castelao, Lucía L Senin, María O Landrove, Javier Baltar, Ana Belén Crujeiras, et al. FNDC5/irisin is not only a myokine but also an adipokine. PLoS One. 2013; 8(4): e60563.doi: 10.1371/journal.pone.0060563. [DOI:10.1371/journal.pone.0060563] [PubMed] [Google Scholar]
36. 36 - Sanchis-Gomar F, Lippi G, Mayero S, Perez-Quilis C, García-Giménez JL. Irisin: a new potential hormonal target for the treatment of obesity and type 2 diabetes. J Diabetes 2012; 4: 196. J Diabetes. 2012; 4(3): 196.doi: 10.1111/j.1753-0407.2012.00194.x. [DOI:10.1111/j.1753-0407.2012.00194.x] [PubMed] [Google Scholar]
37. 37 - Wilson B, Liotta LA, Petricoiniii E. Dynamic protein pathway activation mapping of adipose-derived stem cell differentiation implicates novel regulators of adipocyte differentiation. Molecular & cellular proteomics: MCP. 2013; 12(9): 2522-35. [DOI:10.1074/mcp.M112.025346] [PubMed] [Google Scholar]
38. 38 - Garcia Villar-Cheda B, Sousa-Ribeiro D, Rodriguez-Pallares J, Rodriguez-Perez AI, Guerra MJ, Labandeira- JL. Aging and sedentarism decrease vascularization and VEGF levels in the rat substantia nigra. Implications for Parkinson's disease. Journal of Cerebral Blood Flow & Metabolism. 2010; 29(2): 230-234. [DOI:10.1038/jcbfm.2008.127] [PubMed] [Google Scholar]
39. 39 - Zhao Y, Mazzone T. Human cord blood stem cells and the journey to a cure for type 1 diabetes. Autoimmunity reviews. 2010; 10(2): 103-07. [DOI:10.1016/j.autrev.2010.08.011] [PubMed] [Google Scholar]
40. 40 - Kang X, Huadong X, Sasa T, Xiaoyu Z, Zijun D, Li Z. Dopamine release from transplanted neural stem cells in Parkinsonian rat striatum in vivo. Pnas. 2014; 111(44): 15804-15809. [DOI:10.1073/pnas.1408484111] [PubMed] [Google Scholar]
41. 41 - Hashemvarzi SA, Heidarianpour A. Preconditioning Effect of Aerobic Exercise with D3 Vitamin Consumption on VEGF Levels in the 6-OHDA Lesioned Parkinson's disease Rat Model. Sport Physiology. 2016; 8(30): 129-42. [Persian] [Google Scholar]

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