Volume 14, Issue 4 (Jul-Aug 2020)                   mljgoums 2020, 14(4): 46-52 | Back to browse issues page

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Raeisi M, Mirkarimi K, Jannat B, Rahimi Esboei B, Pagheh A S, Mehrbakhsh Z, et al . In Vitro Effect of Some Medicinal Plants on Leishmania major Strain MRHO/IR/75/ER. mljgoums. 2020; 14 (4) :46-52
URL: http://mlj.goums.ac.ir/article-1-1270-en.html
1- Assistant Professor of Food Hygiene and Quality Control, Department of Nutrition, Faculty of Health, Golestan University of Medical Sciences
2- Health Management and Social Development Research Center, Department of Public Health, School of Health, Golestan University of Medical Sciences, Gorgan, Iran
3- Halal Research Center of IRI, Fda, Tehran, Iran
4- Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical, Tehran, Iran
5- Infectious Disease Research Center, Birjand University of Medical Sciences, Birjand, Iran
6- Department of Biostatistics, Faculty of Health, Golestan University of Medical Sciences, Gorgan, Iran
7- Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
8- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Abstract:   (1135 Views)
      Background and objectives: Leishmaniasis is a tropical disease caused by protozoan parasites from the genus Leishmania. In this study, we aimed at investigating the in vitro anti-leishmanial effect of essential oils of Rosmarinus officinalis, Mentha pulegium, Foeniculum vulgare, Lippia citriodora and Pelargonium graveolens.
       Methods: The essential oils were prepared from freshly dried and powdered plants with steam-distilled water. Iranian strain of Leishmania promastigotes was cultured in RPMI medium and the inhibitory effects of different concentrations (25, 32, 62.5, 125, 250, 500 and 1000 μg/ml) of the essential oils were investigated at 24, 48 and 72 hours. The number of live parasites before and after treatment with the essential oils was counted by trypan blue 10% staining and using neobar lam.
      Results: The essential oils significantly decreased the number of promastigotes in a dose-dependent manner (P<0.05). However, the inhibitory effects of F. vulgare and R. officinalis essential oils were more profound compared to other essential oils. Moreover, concentrations of 500 and 1000 μg/ml of these two essential oils exerted equal and more anti-leishmanial potency compared to glucantime, the first-line drug used for treatment of leishmaniasis.
       Conclusion: Based on the results, it is recommended to evaluate the in vivo anti-leishmanial effects of the tested essential oils, particularly F. vulgare and R. officinalis.
Full-Text [PDF 1135 kb]   (202 Downloads)    
Research Article: Original Paper | Subject: Mycology
Received: 2019/12/4 | Accepted: 2019/12/11 | Published: 2020/07/28 | ePublished: 2020/07/28

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