Volume 13, Issue 5 (Sep-Oct 2019)                   mljgoums 2019, 13(5): 13-18 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Elikaei A, Vazini H, Javani Jouni F, Zafari J. Investigating Cytotoxic Effects of Juniperus Excelsa Extract on Esophageal Cancer Cell Line KYSE-30 and Normal Fibroblast Cell Line HU02. mljgoums. 2019; 13 (5) :13-18
URL: http://mlj.goums.ac.ir/article-1-1243-en.html
1- Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran , arezoo_elikaei774@yahoo.com
2- Department of Nursing, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran
3- Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran
Abstract:   (52 Views)
ABSTRACT
             Background and objectives: Esophageal cancer is the eighth most common type of cancer in the world. Considering the adverse effects of anticancer drugs and the emergence of chemotherapy resistance, plant-derived extracts and their constituents could be a valuable source of novel anticancer drugs. In this study, we investigated cytotoxic effects of Juniperus excelsa leaf extract on esophageal cancer cell line KYSE-30 and healthy fibroblast cells (HU02 cells).
             Methods: KYSE-30 cells and HU02 cells were cultured in DMEM medium. The cells were treated with different concentrations (1, 10, 100, 500 μg/ml) of the J. excelsa leaf extract for 24 and 48 hours. The cytotoxic effects of the extract were assessed using the MTT assay. Data were analyzed using SPSS (version 19) and GraphPad Prism 5.
             Results: According to results of the MTT assay, the Juniperus excelsa’s leaf extract exerted significant cytotoxic effects on esophagus cancer cell line (KYSE-30) and healthy fibroblast cells (HU02) in a time- and dose-dependent manner (P<0.05).
             Conclusion: The J. excelsa leaf extract has cytotoxic effects against KYSE-30 esophageal cancer cells while causing lesser toxicity on healthy fibroblast cells. Our findings suggest that the potential anticancer effects of this extract should be further exploited in future studies.
             Keywords: Cytotoxic, MTT, Hu02, Kyse-30, Juniperus excelsa.
Full-Text [PDF 741 kb]   (21 Downloads)    
Type of Study: Original Paper | Subject: Sport Physiology
Received: 2019/08/21 | Accepted: 2019/08/21 | Published: 2019/08/21 | ePublished: 2019/08/21

References
1. Akbari MR, Malekzadeh R, Nasrollahzadeh D, Amanian D, Sun P, Islami F, et al. Familial risks of esophageal cancer among the Turkmen population of the Caspian littoral of Iran. International journal of cancer. 2006; 119(5): 1047-51. [DOI:10.1002/ijc.21906]
2. Stathopoulos G, Tsiaras N. Epidemiology and pathogenesis of esophageal cancer: management and its controversial results. Oncol Rep. 2003; 10(2): 449-54. [DOI:10.3892/or.10.2.449]
3. Sadjadi A, Nouraie M, Mohagheghi MA, Mousavi-Jarrahi A, Malekezadeh R, Parkin DM. Cancer occurrence in Iran in 2002, an international perspective. Asian Pac J Cancer Prev. 2005; 6(3): 359-63.
4. Zhang L, Wu YD, Li P, Tu J, Niu YL, Xu CM, Zhang ST. Effects of cyclooxygenase-2 on human esophageal squamous cell carcinoma.World J Gastroenterol. 2011; 17(41): 4572-80. doi: 10.3748/wjg.v17.i41.4572. [DOI:10.3748/wjg.v17.i41.4572]
5. Nouri Dalouyi M, Mahrerallnaghsh R, Sayyah MK. Molecular genetics and gene therapy in esophageal cancer: a review article. Tehran University Medical Journal. 2011; 69(6): 331-343. [Persian]
6. Pabla N, Murphy RF, Liu K, Dong Z. The copper transporter Ctr1 contributes to cisplatin uptake by renal tubular cells during cisplatin nephrotoxicity. Am J Physiol Renal Physiol. 2009; 296(3): F505-11. doi: 10.1152/ajprenal.90545.2008. [DOI:10.1152/ajprenal.90545.2008]
7. Rabik CA, Dolan ME. Molecular mechanisms of resistance and toxicity associated with platinating agents. Cancer treatment reviews. 2007; 33(1): 9-23. [DOI:10.1016/j.ctrv.2006.09.006]
8. Kawashty SA, Mosharrata SAM, El-Gibali M, Saleh NAM. The flavonoids of four Pistacia species in Egypt. Biochem Syst Ecol. 2000; 28(9): 915-7. [DOI:10.1016/S0305-1978(99)00113-1]
9. Emami S.A, Abedindoa B.F , Hassanzadeh-Khayya M, Antioxidant Activity of the Essential Oils of Different Parts of Juniperus excelsa M. Bieb. Subsp. excelsa and J. excelsa M. Bieb. Subsp. polycarpos (K. Koch) Takhtajan (Cupressaceae). Iranian Journal of Pharmaceutical Research. 2011; 10 (4): 799-810. DOI: 10.22037/ijpr.2011.985 .
10. Yesilada E, Honda G, Sezik E, Tabata M, Fujita T, et al. Traditional medicine in Turkey. V. Folk medicine in the inner Taurus Mountains. J. Ethnopharmacol. 1995; 46(3): 133-152. [DOI:10.1016/0378-8741(95)01241-5]
11. Fujita T, Sezik E, Tabata M, Yesilada E, Honda G, Takeda Y. Traditional medicine in Turkey VIIl. Folk medicine in middle and west black sea regions. Econ. Bot. 1995; 49: 406-422. [DOI:10.1007/BF02863092]
12. Forouzandeh F, Salimi S, Naghsh N, Zamani N, Jahani S. Evaluation of anti-cancer effect of Peganum harmala L hydroalcholic extract on human cervical carcinoma epithelial cell line (HeLa) Journal of Shahrekord University of Medical Sciences. 2014; 16(4): 1-8
13. Moheghi1 N, Tavakkol Afshari J, Brook A. The Cytotoxic Effect of Zingiber Afficinale in Breast Cancer Cell Line (MCF7) Ofogh-e-Danesh; Journal of Gonabad University of Medical Sciences. 2011; 17(4): 28-34. [Persian]
14. Meimandi K, yaghoobi MM. Effects of aqueous and ethanolic extract of Rosa damascena mill L.against human gastric Cancer cells, Journal of Molecular and Cellular Research (Iranian Journal of Biology). 2015; 28(2): 299-309. [Persian]
15. Behdarvand Shoushtar A, Sazgar H, Ghasemi Pirbaloti A. Investigating cytotoxic effect of hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge on Hela cancer cells line. Medical Sciences. 2017; 27(3): 178-186. [Persian]
16. Nejad Shahrokhabadi K, Tavakkol Afshari J, Rakhshandeh H, Barouk A. Study Of cytotoxicity effect of total saffron extract on hepatocarcinoma cell line (HepG2). Medical Sciences. 2009; 19(3): 154-159.[Persian]
17. Seyedalipour B, Pourakbar E, Taravati A. The Cytotoxic Effect of Ethanolic Extract of Pistacia Khinjuk Leaf on HeLa and MCF-7 Cancerous Cell Lines. J RafsanjanUniv Med Sci. 2016; 14(11): 939-52. [Persian]
18. Zamanian Azodi M, Heydari Keshel S, Azizi Jalilian F. Evaluation of the Ctytotoxic Effect of Cuminum cyminum essential oils on Human Colon Cancer cell line SW742. sjimu. 2013; 20(5): 138-148.[Persian]
19. Bahluli Sh, Rostami R, Jahed Sh, Hossein Zadeh Sh. Investigating the antinociceptive effect of Ecballium Elaterium on the esophageal cell carcinoma cell line (KYSE-30). 11th Annual Conference of Iranian Pathology Society and 5th annual Cancer Society Congress, Tehran, Iran. October 2009; 28-30.
20. Vahedi Larijani L, Ghasemi M, Abediankenari S, Naghshvar F, Azadbakht M, Yazdani Cherati J, et al. The Study of the Effects of Four Avocado Extracts in Esophagus Squamous Cell Carcinoma. J Mazand Univ Med Sci. 2013; 23(2): 48-54. [Persian]
21. Khabbaz Azar S, Moeen MR, Niknahad H. Cytotoxicity of Juniperus excels and Salvia mirzayanii Extracts against HepG2 Cells. Iranian Journal of Pharmaceutical Sciences Autumn. 2012; 8(4): 241-247.
22. Topcu G, Erenler R, Cakmak O, Johansson CB, Celik C, Chai HB, Pezzuto JM. Diterpenes from the berries of Juniperus excelsa. Phytochemistry. 1999; 50(7):1195-9. [DOI:10.1016/S0031-9422(98)00675-X]
23. Sadeghi-aliabadi H, Emami A, Saidi M, Sadeghi B, Jafarian A. Evaluation of In Vitro Cytotoxic Effects of Juniperus foetidissima and Juniperus sabina Extracts Against a Panel of Cancer Cells. Iranian Journal of Pharmaceutical Research. 2009; 8(4): 281-286. DOI: 10.22037/ijpr.2010.823.
24. Muto N, Tomokuni T, Haramoto M, Tatemoto H,Nakanishi T, Inatomi Y, et al. Isolation of apoptosis- and differentiation-inducing substances toward human promyelocytic leukemia HL-60 cells from leaves of Juniperus taxifolia. Biosci Biotechnol Biochem. 2008; 72(2):477-84. [DOI:10.1271/bbb.70570]
25. Emami SA, Asili J, Mohagheghi Z, Hassanzadeh MK. Antioxidant activity of leaves and fruits of Iranian conifers. Ecam. 2007; 4(3): 313-9. [DOI:10.1093/ecam/nem011]
26. Hotta M, Nakata R, Katsukawa M, Hori K, Takahashi S, Hiroyasu I. Carvacrol, a component of thyme oil, activates PPARα and γ and suppresses cox- 2 expression. J Lipid Res. 2010; 51(1): 132-9. doi: 10.1194/jlr.M900255-JLR200. [DOI:10.1194/jlr.M900255-JLR200]
27. Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF. Bioactive compounds in foods:their role in the prevention of cardiovascular disease and cancer. AM J MED. 2002; 113(Suppl 9): 71-88. [DOI:10.1016/S0002-9343(01)00995-0]
28. Kumaran A, Karunakaran RJ. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem. 2006; 97(1): 109-114. [DOI:10.1016/j.foodchem.2005.03.032]
29. Pokorny J. Are Natural Antioxidants etter and Safer than Synthetic Antioxidant Components. Eur J Lipid Sci Technol. 2007; 109(9): 629-42. DOI: 10.1002/ejlt.200700064. [DOI:10.1002/ejlt.200700064]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2007 All Rights Reserved | Medical Laboratory Journal