Volume 14, Issue 2 (Mar-Apr 2020)                   mljgoums 2020, 14(2): 31-35 | Back to browse issues page

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tajaldini M, Samadi F, Khosravi A, Ghasemnejad A, Asadi J. Inhibition of Growth and Migration of Esophageal Squamous Cell Carcinoma Cells by Orange Peel Extract and Naringin. mljgoums. 2020; 14 (2) :31-35
URL: http://mlj.goums.ac.ir/article-1-1238-en.html
1- Department of Animal and Poultry Physiology, Faculty of Animal Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran , tajodini1363@yahoo.com
2- Department of Animal and Poultry Physiology, Faculty of Animal Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3- Department of Molecular Medicine, Faculty of Medical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
4- Department of horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
5- Metabolic Disorder Center, Golestan University of Medical Sciences, Gorgan, Iran
Abstract:   (1004 Views)
            Background and Objectives: Citrus fruits and their constituents especially naringin (NR), a natural predominant flavanone, have a wide range of pharmacological activities without toxicity against cancer cells. The aim of this study was to investigate the anticancer effects of orange peel extract (OPE) and naringin (NR) on esophageal squamous cell carcinoma (ESCC) cells.
            Methods: Amount of phenol, flavonoid and antioxidants in OPE was determined using Folin-Ciocalteu procedure, aluminum chloride colorimetric and DPPH assays, respectively. Effects of NR and OPE on viability, wound healing assay and DNA fragmentation using DAPI were investigated. Data were analyzed by ImageJ software and GraphPad Prism 6.0 at significance of 0.05.
            Results: Total amount of phenols, flavonoids and 1,1-diphenyl-2-picrylhydrazyl was 2.83, 2.143 and 60.76 g/100g of OPE. Amount of NR in the dried OPE was estimated to be 5.260 (µg/gr) using high-performance liquid chromatography. Treatment of ESCC cells with OPE or NR decreased viability y of cancer cells in a dose-dependent manner. In addition, both OPE and NR were able to decrease cell migration and increase DNA fragmentation.
            Conclusion: The findings of our study suggest that OPE and NR have anticancer effects on ESCC cells but the anticancer effects of OPE was better than that of NR alone.
            Keywords: Orange peel extract, Naringin, Migration, Esophageal squamous cell carcinoma.

Full-Text [PDF 657 kb]   (83 Downloads)    
Type of Study: Original Paper | Subject: Herbals
Received: 2019/08/7 | Accepted: 2019/08/10 | Published: 2020/03/12 | ePublished: 2020/03/12

1. Available online: http://www.who.int/mediacentre/factshee ts/fs297/en/ (accessed on 1 February 2018) [press release]. 2018.
2. Pennathur A, Gibson MK, Jobe BA, Luketich JD. Oesophageal carcinoma. The Lancet. 2013; 381(9864): 400-12. [DOI:10.1016/S0140-6736(12)60643-6]
3. Atanasov AG, Waltenberger B, Pferschy-Wenzig E-M, Linder T, Wawrosch C, Uhrin P, et al. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnology advances. 2015; 33(8): 1582-614. [DOI:10.1016/j.biotechadv.2015.08.001]
4. Amaral R, dos Santos S, Andrade L, Severino P, Carvalho A. Natural Products as Treatment against Cancer: A Historical and Current Vision. Clin Oncol. 2019; 4: 1562.
5. Fimognari C, Hrelia P. Sulforaphane as a promising molecule for fighting cancer. Mutat Res. 2007; 635(2-3): 90-104. [DOI:10.1016/j.mrrev.2006.10.004]
6. Wang H, Oo Khor T, Shu L, Su Z-Y, Fuentes F, Lee J-H, et al. Plants vs. cancer: a review on natural phytochemicals in preventing and treating cancers and their druggability. Anticancer Agents Med Chem. 2012; 12(10): 1281-305. [DOI:10.2174/187152012803833026]
7. Rafiq S, Kaul R, Sofi S, Bashir N, Nazir F, Nayik GA. Citrus peel as a source of functional ingredient: A review. Journal of the Saudi Society of Agricultural Sciences. 2018; 17(4): 351-8. [DOI:10.1016/j.jssas.2016.07.006]
8. Liu X, Lin C, Ma X, Tan Y, Wang J, Zeng M. Functional characterization of a flavonoid glycosyltransferase in sweet orange (Citrus sinensis). Front Plant Sci. 2018; 9: 166. doi: 10.3389/fpls.2018.00166. [DOI:10.3389/fpls.2018.00166]
9. Murthy KC, Jayaprakasha G, Patil BS. Obacunone and obacunone glucoside inhibit human colon cancer (SW480) cells by the induction of apoptosis. Food Chem Toxicol. 2011; 49(7): 1616-25. doi: 10.1016/j.fct.2011.04.014. [DOI:10.1016/j.fct.2011.04.014]
10. Banjerdpongchai R, Wudtiwai B, Khaw-on P, Rachakhom W, Duangnil N, Kongtawelert P. Hesperidin from Citrus seed induces human hepatocellular carcinoma HepG2 cell apoptosis via both mitochondrial and death receptor pathways. Tumour Biol. 2016; 37(1): 227-37. doi: 10.1007/s13277-015-3774-7. [DOI:10.1007/s13277-015-3774-7]
11. Tajaldini M, Samadi F, Khosravi A, Ghasemnejad A, Asadi J. Protective and anticancer effects of orange peel extract and naringin in doxorubicin treated esophageal cancer stem cell xenograft tumor mouse model. Biomedicine & Pharmacotherapy. 2020; 121: 109594. [DOI:10.1016/j.biopha.2019.109594]
12. Wang R, Wang Y, Gao Z, Qu X. The comparative study of acetyl-11-keto-beta-boswellic acid (AKBA) and aspirin in the prevention of intestinal adenomatous polyposis in APCMin/+ mice. Drug Discov Ther. 2014; 8(1): 25-32. [DOI:10.5582/ddt.8.25]
13. Arawande J, Komolafe E. Antioxidative potential of banana and plantain peel extracts on crude palm oil. Ethnotanical Leaflet. 2010;14: 559-69.
14. Pothitirat W, Gritsanapan W. Assessment of the phenolic content and free radical scavenging capacity of extracts obtained from the pericarp of Garcinia mangostana L. Planta Medica. 2010; 76(12): SL_46. DOI: 10.1055/s-0030-1264284. [DOI:10.1055/s-0030-1264284]
15. Ayyoob K, Masoud K, Vahideh K, Jahanbakhsh A. Authentication of newly established human esophageal squamous cell carcinoma cell line (YM-1) using short tandem repeat (STR) profiling method. Tumour Biol. 2016; 37(3): 3197-204. doi: 10.1007/s13277-015-4133-4. [DOI:10.1007/s13277-015-4133-4]
16. Karakaş D, Ari F, Ulukaya E. The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. Turk J Biol. 2017; 41(6): 919-925. doi: 10.3906/biy-1703-104. [DOI:10.3906/biy-1703-104]
17. Poon PY, Yue PYK, Wong RNS. A device for performing cell migration/wound healing in a 96-well plate. J Vis Exp. 2017; 121: 55411. doi: 10.3791/55411. [DOI:10.3791/55411]
18. Yoo KM, Hwang IK, Moon B. Comparative Flavonoids Contents of Selected Herbs and Associations of Their Radical Scavenging Activity with Antiproliferative Actions in V79‐4 Cells. J Food Sci. 2009; 74(6): C419-25. doi: 10.1111/j.1750-3841.2009.01191.x. [DOI:10.1111/j.1750-3841.2009.01191.x]
19. Alam MA, Subhan N, Rahman MM, Uddin SJ, Reza HM, Sarker SD. Effect of citrus flavonoids, naringin and naringenin, on metabolic syndrome and their mechanisms of action. Adv Nutr. 2014; 5(4): 404-17. doi: 10.3945/an.113.005603. [DOI:10.3945/an.113.005603]
20. Gattuso G, Barreca D, Gargiulli C, Leuzzi U, Caristi C. Flavonoid composition of citrus juices. Molecules. 2007; 12(8): 1641-73. [DOI:10.3390/12081641]
21. Tripoli E, La Guardia M, Giammanco S, Di Majo D, Giammanco M. Citrus flavonoids: Molecular structure, biological activity and nutritional properties: A review. DOI: 10.1016/j.foodchem.2006.11.054. [DOI:10.1016/j.foodchem.2006.11.054]
22. Castellone RD, Leffler NR, Dong L, Yang LV. Inhibition of tumor cell migration and metastasis by the proton-sensing GPR4 receptor. Cancer Lett. 2011 Dec 22;312(2):197-208. doi: 10.1016/j.canlet.2011.08.013. [DOI:10.1016/j.canlet.2011.08.013]
23. Tsai H-C, Li Y-C, Hsu S-H, Young T-H, Chen M-H. Inhibition of growth and migration of oral and cervical cancer cells by citrus polyphenol. J Formos Med Assoc. 2016; 115(3): 171-85. doi: 10.1016/j.jfma.2015.01.020. [DOI:10.1016/j.jfma.2015.01.020]
24. Lewinska A, Siwak J, Rzeszutek I, Wnuk M. Diosmin induces genotoxicity and apoptosis in DU145 prostate cancer cell line. Toxicol In Vitro. 2015; 29(3): 417-25. doi: 10.1016/j.tiv.2014.12.005. [DOI:10.1016/j.tiv.2014.12.005]
25. Ramesh E, Alshatwi AA. Naringin induces death receptor and mitochondria-mediated apoptosis in human cervical cancer (SiHa) cells. Food Chem Toxicol. 2013; 51: 97-105. doi: 10.1016/j.fct.2012.07.033. [DOI:10.1016/j.fct.2012.07.033]

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