Volume 13, Issue 3 (May-Jun 2019)                   mljgoums 2019, 13(3): 31-34 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jomehpour N, Ghazvini K, Jomehpour M. Antibacterial Activity of Aqueous and Methanolic Extracts of Crocus sativus Stigma and Cinnamomum cassia against Clinical Isolates of some Gram-Positive and Gram-Negative Pathogenic Bacteria. mljgoums. 2019; 13 (3) :31-34
URL: http://mlj.goums.ac.ir/article-1-1209-en.html
1- Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , Jomehpourn961@mum.ac.ir
2- Antimicrobial Resistance Research Center, Buali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran, and Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3- Lab Sciences, Shahrood University of Medical Sciences
Abstract:   (1484 Views)
ABSTRACT
            Background and Objectives: Medicinal and aromatic plants are sources of natural antimicrobial compounds that could be useful replacements for antibiotics. The aim of this study was to assess antimicrobial activity of Crocus sativus stigma and Cinnamomum cassia extracts against some Gram-positive and Gram-negative bacteria.
            Methods: Antimicrobial activity of methanolic and aqueous extracts of the plants was tested against clinical isolates of Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Enterococcus using the microdilution method. Minimal inhibitory concentration (MIC) and minimum bactericidal concentration of each extract against the mentioned bacteria were also determined.
            Results: The MIC of the methanolic extract of C. cassia was 80 µg/ml against Enterococcus, K. pneumonia and E. coli. The MIC of the methanolic extract of C. sativus was 160 µg/ml against Enterococcus and S. aureus. The minimum bactericidal concentration of the methanolic extracts of C. sativus and C. cassia was 320 µg/ml against K. pneumonia and 160 µg/ml against Enterococcus.
            Conclusion: The extracts of C. sativus and C. cassia exhibit promising antibacterial activities against clinical isolates of the tested bacteria. Our results suggest that the extract of these plants can be further exploited as potential antibacterial agents against multi-drug resistant bacteria.
            Keywords: Cinnamomum aromaticum, Crocus, Anti-Bacterial Agents.
Full-Text [PDF 629 kb]   (168 Downloads)    
Type of Study: Original Paper | Subject: Sport Physiology
Received: 2019/04/23 | Accepted: 2019/04/23 | Published: 2019/04/23 | ePublished: 2019/04/23

References
1. Soniya M, Kuberan T, Anitha S, Sankareswari P. In vitro antibacterial activity of plant extracts against Gram positive and Gram negative pathogenic bacteria. International Journal of Microbiology and Immunology Research. 2013; 2(1): 1-5.
2. Bidlack WR, Omaye ST, Meskin MS, Topham DK. Phytochemicals as bioactive agents: CRC press. 2000. [DOI:10.1201/9781482278880]
3. Djeussi DE, Noumedem JA, Seukep JA, Fankam AG, Voukeng IK, Tankeo SB, et al. Antibacterial activities of selected edible plants extracts against multidrug-resistant Gram-negative bacteria. BMC Complement Altern Med. 2013; 13: 164. doi: 10.1186/1472-6882-13-164. [DOI:10.1186/1472-6882-13-164]
4. Al-Mariri A, Safi M. In vitro antibacterial activity of several plant extracts and oils against some gram-negative bacteria. Iran J Med Sci. 2014; 39(1) :36-39.
5. Sumitra C, Yogesh B. Extraction of active compounds of some medical plants. African journal of Biotechnology. 2010;9:3210-7.
6. Vahidi H, Kamalinejad M, Sedaghati N. Antimicrobial properties of Croccus sativus L. Iranian Journal of Pharmaceutical Research. 2002; 1(2): 33-5.
7. Okmen G, Kardas S, Bayrak D, Arslan A, Cakar h. the antibacterial activities of crocus sativus against mastitis pathogens and its antioxidant activities. world journal of pharmacy and pharmaceutical sciences. 2016; 5(3):146-156
8. Zhang Y, Liu X, Wang Y, Jiang P, Quek S. Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus. Food Control. 2016; 59: 282-9. [DOI:10.1016/j.foodcont.2015.05.032]
9. Buru AS, Pichika MR, Neela V, Mohandas K. In vitro antibacterial effects of Cinnamomum extracts on common bacteria found in wound infections with emphasis on methicillin-resistant Staphylococcus aureus. Journal of ethnopharmacology. 2014; 153(3): 587-95. [DOI:10.1016/j.jep.2014.02.044]
10. Li Y-q, Kong D-x, Wu H. Analysis and evaluation of essential oil components of cinnamon barks using GC-MS and FTIR spectroscopy. Industrial Crops and Products. 2013; 41: 269-78. [DOI:10.1016/j.indcrop.2012.04.056]
11. Clinical and Laboratory Standard Institute(CLSI). Performance Standards for Antimicrobial Susceptibility Testing: Twenty First Informational Supplement. CLSI document M100-S21. 2011.
12. Sette L, Passarini MR, Delarmelina C, Salati F, Duarte MC. Molecular characterization and antimicrobial activity of endophytic fungi from coffee plants. World Journal of Microbiology and Biotechnology. 2006; 22(11): 1185-95. [DOI:10.1007/s11274-006-9160-2]
13. Asgarpanah J, Darabi-Mahboub E, Mahboubi A, Mehrab R, Hakemivala M. In-Vitro Evaluation of Crocus Sativus L. Petals and Stamens as Natural Antibacterial Agents Against Food-Borne Bacterial Strains. Iranian Journal of Pharmaceutical Sciences. 2013; 9(4): 69-82.
14. Parray JA, Kamili AN, Hamid R, Reshi ZA, Qadri RA. Antibacterial and antioxidant activity of methanol extracts of Crocus sativus L. cv. Kashmirianus. Frontiers in Life Science. 2015; 8(1): 40-6. [DOI:10.1080/21553769.2014.951774]
15. Pintado C, de Miguel A, Acevedo O, Nozal L, Novella JL, Rotger R. Bactericidal effect of saffron (Crocus sativus L.) on Salmonella enterica during storage. Food Control. 2011; 22(3): 638-42. [DOI:10.1016/j.foodcont.2010.09.031]
16. Acar G, Dogan NM, Duru ME, Kıvrak I. Phenolic profiles antimicrobial and antioxidant activity of the various extracts of Crocus species in Anatolia. African Journal of Microbiology Research. 2010; 4(11): 1154-61.
17. Carmona M, Zalacain A, Salinas M, Alonso G. A new approach to saffron aroma. Critical Reviews in Food Science and Nutrition. 2007; 47(2): 145-59. [DOI:10.1080/10408390600626511]
18. Gupta C, Garg AP, Uniyal RC, Kumari A. Comparative analysis of the antimicrobial activity of cinnamon oil and cinnamon extract on some food-borne microbes. African Journal of Microbiology Research. 2008; 2(9): 247-51.
19. Mukhtar S, Ghori I. Antibacterial activity of aqueous and ethanolic extracts of garlic, cinnamon and turmeric against escherichia coli ATCC 25922 and bacillus subtilis DSM 3256. International Journal of Applied Biology and Pharmaceutical Technology. 2012; 3(2): 131-136.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2007 All Rights Reserved | Medical Laboratory Journal