Volume 13, Issue 1 (Jan-Feb 2019)                   mljgoums 2019, 13(1): 21-27 | Back to browse issues page


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Mohadjerani M, Damanjany M. Protective Effect of Ilex spinigera and Gleditsia caspica Extracts against Drug-Induced Hemolysis in Glucose-6-phosphate dehydrogenase-deficient Patients. mljgoums. 2019; 13 (1) :21-27
URL: http://mlj.goums.ac.ir/article-1-1155-en.html
1- Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Abstract:   (2223 Views)
ABSTRACT
           Background and Objectives: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common X-linked disorder of human erythrocytes in which cells are susceptible towards hemolytic changes and could be destroyed by peroxides. Extracts of Ilex spinigera and Gleditsia caspica leaves have excellent free radical scavenging activity. We investigated the protective effects of these extracts against hemolysis induced by some drugs in G6PD-deficient erythrocytes.
           Methods: Blood samples were collected from males with and without G6PD deficiency. Hemolysis induced by aspirin, phenylhydrazine hydrochloride and phenacetin was assessed in the presence and absence of the extracts. The amount of released hemoglobin was determined by reading absorbance at 540 nm.
           Results: The methanol extract of G. caspica had significant protective effects against phenacetin-induced hemolysis in G6PD-deficient human erythrocytes (P<0.05). However, the I. spinigera extract had no significant anti-hemolytic effects on these cells.
           Conclusion: Our findings suggest that the extract of G. caspica could be a potential drug with antioxidant and anti-hemolytic properties for patients with G6PD deficiency.
           Keywords: Antihemolytic activity, Medicinal plant, G6PD deficiency, Gleditsia caspica, Ilex spinigera.
Full-Text [PDF 682 kb]   (525 Downloads)    
Type of Study: Original Paper |
Received: 2018/12/15 | Accepted: 2018/12/15 | Published: 2018/12/15 | ePublished: 2018/12/15

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