Volume 13, Issue 5 (Sep-Oct 2019)                   mljgoums 2019, 13(5): 32-37 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Keyghobadi F, Bahramifar N, Gharekhani E, Kia M. Optimization of Low Amounts of Selenium Ions in Water and Blood Samples by Solid Phase Extraction Using Thiolated Nanosilica. mljgoums. 2019; 13 (5) :32-37
URL: http://mlj.goums.ac.ir/article-1-1131-en.html
1- (MSc) Department of Analytical Chemistry, Golestan University of Medical Sciences, Gorgan, Iran , fereshteh_keyghobadi@yahoo.com
2- (PhD) Department of Analytical Chemistry, Tarbiat Modares University, Noor, Iran
3- (MSc) Department of Toxicology, Golestan University of Medical Sciences, Gorgan, Iran
4- (MSc) Department of Food Sciences, Golestan University of Medical Sciences, Gorgan, Iran
Abstract:   (342 Views)
             Background and Objectives: In this study, nanosilica modified with HS-SiO2 thiol groups was utilized as adsorbent for solid phase extraction, as a fast and reliable method of preconcentration and separation of very small quantities of selenium ions from water and blood samples.
             Methods: The samples included four natural water samples and one biological sample (blood serum) prepared in volumes of 25, 100, 200, 300, 400 and 500 ml. The samples were analyzed by solid phase microextraction, using thiolated-nanosilica (as adsorbent), ultraviolet-visible spectrophotometry and atomic absorption spectroscopy.
             Results: Optimized conditions for preconcentration of a 25 ml 0.2 mg/l selenium solution were pH 5, 40 mg of adsorbent, sample-adsorbent mixing time of 15 minutes and 5 ml of 2N sulfuric acid as detergent. The volume limit and concentration factor were 400 and 80, respectively. Limit of detection and relative standard deviation of the method were 0.46 μg/l and 0.9%, respectively.
             Conclusion: This study is the first to successfully utilize thiolated nanosilica for measuring low selenium levels. Thiolation of the absorbent increases selenium adsorption by thiolated-silica compared to SiO2.
             Keywords: Solid phase extraction, Selenium, Preconcentration, Nano, UV-visible spectrophotometry.
Full-Text [PDF 595 kb]   (48 Downloads)    
Type of Study: Original Paper | Subject: Biochemistry
Received: 2018/10/31 | Accepted: 2019/01/13 | Published: 2019/09/2 | ePublished: 2019/09/2

1. Li T, Yu L-J, Li M-T, Li WJMa. A new approach to the standard addition method for the analysis of F, Al and K content in green tea. 2006;153(1-2):109-14. [DOI:10.1007/s00604-005-0454-0]
2. Sunde RAJAron. Molecular biology of selenoproteins. 1990;10(1):451-74. [DOI:10.1146/annurev.nutr.10.1.451]
3. Hamilton SJJSotTE. Review of selenium toxicity in the aquatic food chain. 2004;326(1-3):1-31. [DOI:10.1016/j.scitotenv.2004.01.019]
4. Yang G, Zhou RJJote, health ei, disease. Further observations on the human maximum safe dietary selenium intake in a seleniferous area of China. 1994;8(3-4):159-65.
5. Grant K, Goldizen FC, Sly PD, Brune M-N, Neira M, van den Berg M, et al. Health consequences of exposure to e-waste: a systematic review. 2013;1(6):e350-e61. [DOI:10.1016/S2214-109X(13)70101-3]
6. Rahman I, Vejayakumaran P, Sipaut C, Ismail J, Bakar MA, Adnan R, et al. An optimized sol-gel synthesis of stable primary equivalent silica particles. 2007;294(1-3):102-10. [DOI:10.1016/j.colsurfa.2006.08.001]
7. Dalvie MA, Sinanovic E, London L, Cairncross E, Solomon A, Adam HJEr. Cost analysis of ELISA, solid-phase extraction, and solid-phase microextraction for the monitoring of pesticides in water. 2005;98(1):143-50. [DOI:10.1016/j.envres.2004.09.002]
8. McKittrick MW, Jones CWJCom. Toward single-site functional materials preparation of amine-functionalized surfaces exhibiting site-isolated behavior. 2003;15(5):1132-9. [DOI:10.1021/cm020952z]
9. Sabik H, Jeannot R, Rondeau BJJocA. Multiresidue methods using solid-phase extraction techniques for monitoring priority pesticides, including triazines and degradation products, in ground and surface waters. 2000;885(1-2):217-36. [DOI:10.1016/S0021-9673(99)01084-5]
10. Najafi M, Rostamian R, Rafati AJCej. Chemically modified silica gel with thiol group as an adsorbent for retention of some toxic soft metal ions from water and industrial effluent. 2011;168(1):426-32. [DOI:10.1016/j.cej.2010.12.064]
11. Hui K, Chao CYHJJoHM. Synthesis of MCM-41 from coal fly ash by a green approach: Influence of synthesis pH. 2006;137(2):1135-48. [DOI:10.1016/j.jhazmat.2006.03.050]

Add your comments about this article : Your username or Email:

Send email to the article author

© 2007 All Rights Reserved | Medical Laboratory Journal