Volume 13, Issue 6 (Nov-Dec 2019)                   mljgoums 2019, 13(6): 36-43 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirghani S J, Azarbayjani M A, Peeri M, keshtkar A. Investigating Effects of Vitamin D Injection during a Course of Endurance Training On Anthropometrical Parameters of Wistar Rats with High-Fat Diet-Induced Obesity. mljgoums. 2019; 13 (6) :36-43
URL: http://mlj.goums.ac.ir/article-1-1123-en.html
1- Department of Exercise Physiology, Faculty of Physical Education and Sports Science, Islamic Azad University, Central Tehran Branch, Tehran, Iran1
2- Department of Exercise Physiology, Faculty of Physical Education and Sports Science, Islamic Azad University, Central Tehran Branch, Tehran, Iran1 , ali.azarbayjani@gmail.com
3- Department of Health Sciences Education Development, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2
Abstract:   (99 Views)
           Background and Objectives: The purpose of this research was to determine effects of vitamin D supplementation during a course of endurance training on anthropometrical parameters of Wistar rats exposed to a high-fat diet.
Methods: In this experimental study, 38 Wistar rats aged 5-6 weeks were assigned to five groups: 1. Control with normal diet (n=5), 2. Control with high-fat diet (n=5), 3. High-fat diet and vitamin D supplementation (n=10), 4. High-fat diet and endurance training (n=10), and 5. High-fat diet, endurance training and vitamin D supplementation (n=10). The animals were subjected to a high-fat diet (40%) for 13 weeks, followed by 12 weeks of exercise and vitamin D supplementation.
Results: Weight (P=0.02), BMI (P=0.001), Lee index (P=0.01) and energy efficiency (P=0.001) differed significantly between groups 1 and 2. Feed efficiency (P=0.03) and energy efficiency (P=0.01) of groups 2, 3 and 4 were significantly different from that of the control group.
Conclusion: The results of the present study indicate that endurance training and vitamin D supplementation could significantly decrease some anthropometric indices.
Keywords: Endurance Training, Vitamin D, High Fat Diet, Anthropometric Indices.
Full-Text [PDF 736 kb]   (59 Downloads)    
Type of Study: Original Paper |
Received: 2018/09/10 | Accepted: 2019/09/28 | Published: 2019/10/30 | ePublished: 2019/10/30

1. World Health Organization. Comprehensive implementation plan on maternal, infant and young child nutrition. No. WHO/NMH/NHD/14.1. World Health Organization, 2014.‏
2. Slevin E, Truesdale Kennedy M, McConkey R, Livingstone B, Fleming P. Obesity and overweight in intellectual and non-intellectually disabled children. J Intellect Disabil Res. 2014; 58(3): 211-20. doi: 10.1111/j.1365-2788.2012.01615.x. [DOI:10.1111/j.1365-2788.2012.01615.x]
3. Talaei A, Mohamadi kelishadi M, Adgi Z. the effect of vitamin D on insulin resistance in type II diabetic patients. Diabetol Metab Syndr. 2011; 14 (5): 79-84. doi: 10.1186/1758-5996-5-8. [persian] [DOI:10.1186/1758-5996-5-8]
4. Bian S, Gao Y, Zhang M, Wang X, Liu W, Zhang D, et al. Dietary nutrient intake and metabolic syndrome risk in Chinese adults: a case-control study. Nutr J. 2013; 12: 106. doi: 10.1186/1475-2891-12-106. [DOI:10.1186/1475-2891-12-106]
5. Brenner DR, Arora P, Garcia-Bailo B, Wolever TM, Morrison H, El-Sohemy A, et al. Plasma vitamin D levels and risk of metabolic syndrome in Canadians. Clin Invest Med. 2011; 34(6):E377. [DOI:10.25011/cim.v34i6.15899]
6. Mohammadian S, Mortezazadeh R, Zaeri H, Vakili MA. Relationship between 25-hydroxy vitamin-D and obesity in 2-7 years old children referred to a paediatric hospital in Iran. J Clin Diagn Res. 2014; 8(9): PC06-8. doi: 10.7860/JCDR/2014/8282.4810. [DOI:10.7860/JCDR/2014/8282.4810]
7. Shin JY, Xun P, Nakamura Y, He K. Egg consumption in relation to risk of cardiovascular disease and diabetes: a systematic review and meta-analysis. Am J Clin Nutr. 2013; 98(1): 146-59. doi: 10.3945/ajcn.112.051318. [DOI:10.3945/ajcn.112.051318]
8. Swithers SE, Ogden SB, Davidson TL. Fat substitutes promote weight gain in rats consuming high-fat diets. Behav Neurosci. 2011; 125(4): 512-8. doi: 10.1037/a0024404. [DOI:10.1037/a0024404]
9. Williams NH. Promoting physical activity in primary care. BMJ. 2011; 343: d6615. doi: 10.1136/bmj.d6615. [DOI:10.1136/bmj.d6615]
10. Bradley RL, Jeon JY, Liu FF, Maratos-Flier E. Voluntary exercise improves insulin sensitivity and adipose tissue inflammation in diet-induced obese mice. Am J Physiol Endocrinol Metab. 2008; 295(3): E586-94. doi: 10.1152/ajpendo.00309.2007. [DOI:10.1152/ajpendo.00309.2007]
11. Huang P, Li S, Shao M, Qi Q, Zhao F, You J, et al. Calorie restriction and endurance exercise share potent anti-inflammatory function in adipose tissues in ameliorating diet-induced obesity and insulin resistance in mice. Nutr Metab (Lond). 2010; 7: 59. doi: 10.1186/1743-7075-7-59. [DOI:10.1186/1743-7075-7-59]
12. Lin S, Thomas TC, Storlien LH, Huang XF. Development of high fat diet-induced obesity and leptin resistance in C57Bl/6J mice. Int J Obes Relat Metab Disord. 2000; 24(5): 639-46. [DOI:10.1038/sj.ijo.0801209]
13. Pierard M, Conotte S, Tassin A, Boutry S, Uzureau P, Boudjeltia KZ, et al. A Interactions of exercise training and high-fat diet on adiponectin forms and muscle receptors in mice. Nutr Metab (Lond). 2016; 13: 75. doi: 10.1186/s12986-016-0138-2. [DOI:10.1186/s12986-016-0138-2]
14. Ferrante AW Jr. Obesity‐induced inflammation: a metabolic dialogue in the language of inflammation. J Intern Med. 2007; 262(4): 408-14. [DOI:10.1111/j.1365-2796.2007.01852.x]
15. Huang BW, Chiang MT, Yao HT, Chiang W. The effect of high‐fat and high‐fructose diets on glucose tolerance and plasma lipid and leptin levels in rats. Diabetes Obes Metab. 2004; 6(2): 120-6. [DOI:10.1111/j.1462-8902.2004.00323.x]
16. Fontana L, Meyer TE, Klein S, Holloszy JO. Long-term calorie restriction is highly effective in reducing the risk for atherosclerosis in humans. Proc Natl Acad Sci U S A. 2004; 101(17): 6659-63. [DOI:10.1073/pnas.0308291101]
17. Shen Y, Xu X, Yue K, Xu G. Effect of different exercise protocols on metabolic profiles and fatty acid metabolism in skeletal muscle in high‐fat diet‐fed rats. Obesity. 2015; 23(5): 1000-1006. [DOI:10.1002/oby.21056]
18. Mirghani SJ, Peeri M, Yaghoobpour Yekani O, Zamani M, Feizolahi F, Nikbin S, et al. Role or Synergistic Interaction of Adenosine and Vitamin D3 Alongside High-Intensity Interval Training and Isocaloric Moderate Intensity Training on Metabolic Parameters: Protocol for an Experimental Study. JMIR Res Protoc. 2019; 8(1): e10753. doi: 10.2196/10753. [DOI:10.2196/10753]
19. Pereira MG, Ferreira JC, Bueno CR Jr, Mattos KC, Rosa KT, Irigoyen MC, et al. Exercise training reduces cardiac angiotensin II levels and prevents cardiac dysfunction in a genetic model of sympathetic hyperactivity-induced heart failure in mice. Eur J Appl Physiol. 2009; 105(6): 843-50. doi: 10.1007/s00421-008-0967-4. ‏ [DOI:10.1007/s00421-008-0967-4]
20. Haghshenas R, Jafari M, Ravasi A, Kordi M, Gilani N, Shariatzadeh M, et al. The effect of eight weeks endurance training and high-fat diet on appetite-regulating hormones in rat plasma. Iran J Basic Med Sci. 2014; 17(4): 237-43.
21. Elj NE, Lac G, Tabka Z, Gharbi N, Fezaa SE. Effect of Physical Exercise on Reducing Food Intake and Weight Gain. Procedia - Social and Behavioral Sciences. 2011; 30: 2027-31. [DOI:10.1016/j.sbspro.2011.10.392]
22. Mirghani SJ, Alinejad HA, Azarbayjani MA, Mazidi A, Mirghani SA. Influence of strength, endurance and concurrent training on the lipid profile and blood testosterone and cortisol response in young male wrestlers. Baltic Journal Of Health And Physical Activity. 2014; 6(1): 7-16. DOI: 10.2478/bjha-2014-0001. [DOI:10.2478/bjha-2014-0001]
23. Mirghani SJ, Yousefi MS. The effect of interval recovery periods during HIIT on liver enzymes and lipid profile in overweight women. Science & Sports. 2015; 30: 147-154. [DOI:10.1016/j.scispo.2014.09.002]
24. Mirghani SJ, Yousefi MS, Pekkala S, Sharifian S, Beyshami G. Shorter recovery time following high-intensity interval training induced higher body fat loss among overweight women. Sport Sciences for Health. 2019; 15: 157-165. [DOI:10.1007/s11332-018-0505-7]
25. Bi S, Scott KA, Hyun J, Ladenheim EE, Moran TH. Running wheel activity prevents hyperphagia and obesity in Otsuka long-evans Tokushima Fatty rats: role of hypothalamic signaling. Endocrinology. 2005;146(4):1676-85. [DOI:10.1210/en.2004-1441]
26. Chaolu H, Asakawa A, Ushikai M, Li YX, Cheng KC, Li JB, et al. Effect of exercise and high-fat diet on plasma adiponectin and nesfatin levels in mice. Exp Ther Med. 2011; 2(2): 369-73. [DOI:10.3892/etm.2011.199]
27. Rocha-Rodrigues S, Goncalves IO, Beleza J, Ascensao A, Magalhaes J. Physical exercise mitigates high-fat diet-induced adiposopathy and related endocrine alterations in an animal model of obesity. J Physiol Biochem. 2018; 74(2): 235-246. doi: 10.1007/s13105-018-0609-1. [DOI:10.1007/s13105-018-0609-1]
28. Florentin M, Elisaf MS, Mikhailidis DP, Liberopoulos EN. Vitamin D and metabolic syndrome: is there a link? Curr Pharm Des. 2010; 16(30): 3417-34. [DOI:10.2174/138161210793563509]
29. Wimalawansa SJ. Associations of vitamin D with insulin resistance, obesity, type 2 diabetes, and metabolic syndrome. J Steroid Biochem Mol Biol. 2018; 175: 177-189. doi: 10.1016/j.jsbmb.2016.09.017. [DOI:10.1016/j.jsbmb.2016.09.017]
30. Vimaleswaran KS, Berry DJ, Lu C, Tikkanen E, Pilz S, Hiraki LT, et al. Causal relationship between obesity and vitamin D status: bi-directional Mendelian randomization analysis of multiple cohorts. PLoS Med. 2013; 10(2): e1001383. doi: 10.1371/journal.pmed.1001383. [DOI:10.1371/journal.pmed.1001383]
31. Ruiz-Ojeda FJ, Anguita-Ruiz A, Leis R, Aguilera CM. Genetic Factors and Molecular Mechanisms of Vitamin D and Obesity Relationship. Ann Nutr Metab. 2018; 73(2): 89-99. doi: 10.1159/000490669. [DOI:10.1159/000490669]
32. Kayaniyil S, Vieth R, Harris SB, Retnakaran R, Knight JA, Gerstein HC, et al. Association of 25(OH)D and PTH with metabolic syndrome and its traditional and nontraditional components. J Clin Endocrinol Metab. 2011; 96(1): 168-75. doi: 10.1210/jc.2010-1439. [DOI:10.1210/jc.2010-1439]
33. Pereira-Santos M, Costa PR, Assis AM, Santos CA, Santos DB. Obesity and vitamin D deficiency: a systematic review and meta-analysis. Obes Rev. 2015; 16(4): 341-9. doi: 10.1111/obr.12239. [DOI:10.1111/obr.12239]
34. Yao Y, Zhu L, He L, Duan Y, Liang W, Nie Z, et al. A meta-analysis of the relationship between vitamin D deficiency and obesity. Int J Clin Exp Med. 2015; 8(9): 14977-14984.
35. Wortsman J, Matsuoka LY, Chen TC, Lu Z, Holick MF. Decreased bioavailability of vitamin D in obesity. Am J Clin Nutr. 2000; 72(3): 690-3. [DOI:10.1093/ajcn/72.3.690]
36. Wong KE, Kong J, Zhang W, Szeto FL, Ye H, Deb DK, et al. Targeted expression of human vitamin D receptor in adipocytes decreases energy expenditure and induces obesity in mice. J Biol Chem. 2011; 286(39): 33804-10. doi: 10.1074/jbc.M111.257568. [DOI:10.1074/jbc.M111.257568]
37. Hjelmesaeth J, Hofso D, Aasheim ET, Jenssen T, Moan J, Hager H, et al. Parathyroid hormone, but not vitamin D, is associated with the metabolic syndrome in morbidly obese women and men: a cross-sectional study. Cardiovasc Diabetol. 2009; 8: 7. doi: 10.1186/1475-2840-8-7. [DOI:10.1186/1475-2840-8-7]
38. Karefylakis C, Sarnblad S, Ariander A, Ehlersson G, Rask E, Rask P. Effect of Vitamin D supplementation on body composition and cardiorespiratory fitness in overweight men-a randomized controlled trial. Endocrine. 2018; 61(3): 388-397. doi: 10.1007/s12020-018-1665-6. [DOI:10.1007/s12020-018-1665-6]
39. Ping-Delfos WC, Soares M. Diet induced thermogenesis, fat oxidation and food intake following sequential meals: influence of calcium and vitamin D. Clin Nutr. 2011; 30(3): 376-83. doi: 10.1016/j.clnu.2010.11.006. [DOI:10.1016/j.clnu.2010.11.006]
40. Mason C, Xiao L, Imayama I, Duggan C, Wang CY, Korde L, et al. Vitamin D3 supplementation during weight loss: a double-blind randomized controlled trial. Am J Clin Nutr. 2014; 99(5): 1015-25. doi: 10.3945/ajcn.113.073734. [DOI:10.3945/ajcn.113.073734]
41. Zittermann A, Frisch S, Berthold HK, Gotting C, Kuhn J, Kleesiek K, et al. Vitamin D supplementation enhances the beneficial effects of weight loss on cardiovascular disease risk markers. Am J Clin Nutr. 2009 May;89(5):1321-7. doi: 10.3945/ajcn.2008.27004. [DOI:10.3945/ajcn.2008.27004]
42. Fassina G, Maragno I, Dorigo P, Contessa AR. Effect of vitamin D2 on hormone-stimulated lipolysis in vitro. Eur J Pharmacol. 1969; 5(3): 286-90. [DOI:10.1016/0014-2999(69)90150-2]
43. Pourshahidi LK. Vitamin D and obesity: current perspectives and future directions. Proc Nutr Soc. 2015; 74(2): 115-24. doi: 10.1017/S0029665114001578. [DOI:10.1017/S0029665114001578]

Add your comments about this article : Your username or Email:

Send email to the article author

© 2007 All Rights Reserved | Medical Laboratory Journal