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mljgoums 2018, 12(5): 23-28 |
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Farghi Yamchi A, Dabirzadeh M, Maroufi Y. In Vitro Effect of Methanolic Extract of Quercus infectoria Galls on Promastigotes and Amastigotes of Leishmania major (MRHO/IR/75/ER). mljgoums 2018; 12 (5) :23-28
URL: http://mlj.goums.ac.ir/article-1-1118-en.html
URL: http://mlj.goums.ac.ir/article-1-1118-en.html
1- Department of Parasitology, Faculty of Medical Sciences, Zabol University of Medical Sciences, Zabol, Iran
2- Department of Parasitology & Mycology, Zabol University of Medical Sciences, Zabol, Iran ,dabirzadeh@zbmu.ic.ir
3- Department of Parasitology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Department of Parasitology & Mycology, Zabol University of Medical Sciences, Zabol, Iran ,
3- Department of Parasitology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
Abstract: (12003 Views)
ABSTRACT
Background and objectives: Leishmania major is a flagellated parasitic protozoan that causes cutaneous leishmaniasis. Pentavalent antimony compounds are considered the first-line drugs in the treatment of cutaneous leishmaniasis. However, the use of these drugs is associated with numerous limitations and side effects. Therefore, there is a need for herbal and natural alternatives for these compounds with fewer side effects. In this study, we evaluated the in vitro activity of methanol extract of Quercus infectoria (oak galls) against promastigotes and amastigotes of L. major.
Methods: In this experimental study, the effect of 10, 100, 500 and 1000 µg/ml of methanolic extract of oak galls and 100, 500, 1000 and 10000 µg/ml of Glucantime was evaluated against L. major promastigotes using direct cell counting and MTT assay. Moreover, the effect of different concentrations of the extract and Glucantime was investigated on the mean number of amastigotes in macrophages after 24 and 48 hours. Data were analyzed using SPSS 16 and one- way analysis of variance.
Results: The half-maximal inhibitory concentration of the oak gall extract and Glucantime was 75 µg/ml and 221 µg/ml after 24 hours, respectively. After 24 hours, the mean number of amastigotes per macrophage was lowest at concentrations of 1000 µg/ml of the extract (0.9) and 10000 µg/ml of Glucantime (0.85).
Conclusion: Considering the inhibition of intracellular and extracellular growth of L. major, the oak gall extract might be used as an efficient and safe agent for treatment of cutaneous leishmaniasis.
KEYWORDS: Leishmaniasis, Cutaneous, Quercus.
Background and objectives: Leishmania major is a flagellated parasitic protozoan that causes cutaneous leishmaniasis. Pentavalent antimony compounds are considered the first-line drugs in the treatment of cutaneous leishmaniasis. However, the use of these drugs is associated with numerous limitations and side effects. Therefore, there is a need for herbal and natural alternatives for these compounds with fewer side effects. In this study, we evaluated the in vitro activity of methanol extract of Quercus infectoria (oak galls) against promastigotes and amastigotes of L. major.
Methods: In this experimental study, the effect of 10, 100, 500 and 1000 µg/ml of methanolic extract of oak galls and 100, 500, 1000 and 10000 µg/ml of Glucantime was evaluated against L. major promastigotes using direct cell counting and MTT assay. Moreover, the effect of different concentrations of the extract and Glucantime was investigated on the mean number of amastigotes in macrophages after 24 and 48 hours. Data were analyzed using SPSS 16 and one- way analysis of variance.
Results: The half-maximal inhibitory concentration of the oak gall extract and Glucantime was 75 µg/ml and 221 µg/ml after 24 hours, respectively. After 24 hours, the mean number of amastigotes per macrophage was lowest at concentrations of 1000 µg/ml of the extract (0.9) and 10000 µg/ml of Glucantime (0.85).
Conclusion: Considering the inhibition of intracellular and extracellular growth of L. major, the oak gall extract might be used as an efficient and safe agent for treatment of cutaneous leishmaniasis.
KEYWORDS: Leishmaniasis, Cutaneous, Quercus.
Research Article: Original Paper |
Received: 2018/08/15 | Accepted: 2018/08/15 | Published: 2018/08/15 | ePublished: 2018/08/15
Received: 2018/08/15 | Accepted: 2018/08/15 | Published: 2018/08/15 | ePublished: 2018/08/15
References
1. Kolodzieej H, Kiderlen AF. Anti leishmanial Activity and immune modulatory effects of tannnins and related compounds on Leishmania Parasitised RAW 264.7 Cells. Phytochemistry. 2005; 66(17): 2056-71.
https://doi.org/10.1016/j.phytochem.2005.01.011 [DOI:10.1016/j.phytochem.2005.01.011.]
2. Bermarn JD. Human leishmaniasis: clinical, diagnostic and chemotherapeutic developments in the last 10 years. Clin Infect Dis. 1997; 24(4): 684-703.
https://doi.org/10.1093/clind/24.4.684 [DOI:10.1093/clind/24.4.684.]
3. Kobets T, Grekov I, Lipoldova M. Leishmaniasis: prevention, parasite detection, and treatment. Curr Med Chem. 2012; 19(10):1443-74.
4. Postigo JA. Leishmaniasis in the World Health Organization Eastern Mediterranean Region. Int J Antimicrob Agents. 2010; 36(1): 62-65.
https://doi.org/10.1016/j.ijantimicag.2010.06.023 [DOI:10.1016/j.ijantimicag.2010.06.023.]
5. Bouvier J, Etges R, Bordier C. Identification of the promastigote surface protease in seven species of Leishmania. Mol Biochem Parasitol 1987; 24:73-79.
https://doi.org/10.1016/0166-6851(87)90117-4 [DOI:10.1016/0166-6851(87)90117-4.]
6. Zargaran MR, Safaralizadeh MH, Pourmirza AA, Valizadegan O. Effect of Cardinal Directions on Gall Morphology and Parasitization of the Gall Wasp, Cynips quercus folii. J Insect Sci. 2011; 11: 169.
https://doi.org/10.1673/031.011.16901 [DOI:10.1673/031.011.16901.]
7. Sakar MK, Şöhretoğlu D, Ozalp M, Ekizoğlu M, Placente S, Pizza C. Polyphenolic Compounds and Antimicrobial Activity of Quercus aucheri Leaves.Turk.j.chem.2005; 29: 555-9.
8. Kaur G, Hamid H, Ali A, Alam MS, Athar M. Anti-Inflammatory evaluation of alcoholic extract of galls of Quercus infectoria. J Ethnopharmacol. 2004; 90: 285-92.
https://doi.org/10.1016/j.jep.2003.10.009 [DOI:10.1016/j.jep.2003.10.009.]
9. Kaur G, Athar M, Alam MS. Quercus infectoria galls possess antioxidant activity and abrogate oxidative stress-induced functional alterations in murine macrophages. Elsevier: Chem-Biol Interact. 2008; 171: 272-280.
https://doi.org/10.1016/j.cbi.2007.10.002 [DOI:10.1016/j.cbi.2007.10.002.]
10. Umachigi SP, Jayaveera KN, Ashok Kumar CK, Kumar GS, Vrushabendra swamy BM, Kishore Kumar DV. Studies on Wound Healing Properties of Quercus Infectoria. Trop. J Pharm. Res. 2008; 7(1): 913-9.
https://doi.org/10.4314/tjpr.v7i1.14677 [DOI:10.4314/tjpr.v7i1.14677.]
11. Basri DF, Fan SH. The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents. Indian J Pharmacol. 2004; 37:26-29.
https://doi.org/10.4103/0253-7613.13851 [DOI:10.4103/0253-7613.13851.]
12. Nagesh L, Shayam S, Muralikrishna Ks, Kishore G. Antibacterial potential of gall extract Quercus infectoria against entrococcus faecalis an in vitro study. J Pharmacol. 2012; 30(9): 28-34.
13. Muskhazi M, Nurhafiza y, Nor Azwady AA, Nor Dalilah E. Comparative Study on the In vitro antibacterial efficacy of aqueous and methanolic extracts of Querus infectoria Gall against Cellulosimicrobium cellulanns. Int j Biol Sci. 2008; 8: 634-8.
https://doi.org/10.3923/jbs.2008.634.638 [DOI:10.3923/jbs.2008.634.638.]
14. Sharifi A, Gorjipoura A, Sardsiri M, Mohammadi R, Jabarneyad A. Antifungal effect of Quereus infectoria gall on saprolegnia fungi. Armaghan Danesh. 2012; 17(1): 78-84. [Persian]
15. Nur Saedia B, Hasmah A, Wan Nur Amilah WAW. Anti-Candidia activity of Quercus infectoria gall extracts against Candidia Species. j Pharmacy &Biol Allied Sciences. 2015; 7(1): 15-20.
16. Maroufi y, Ghaffarifar F, Dalimi A, sharifi Z, Hasan Z. Cantharidin –induced apoptosis in Leishmania major Promastigote and macrophages infected by Leishmania major amastigote in vitro.J Mazandaran univ med Sci. 2012; 22(89): 32-40.
17. Chang K T, Dwyer D. Leishmania donovani. Hamster macrophage interactions in vitro: cell entry, intracellular survival, and multiplication of amastigotes. The Journal of experimental medicine. 1978; 147(2): 515-30. PMID:564391.
https://doi.org/10.1084/jem.147.2.515 [DOI:10.1084/jem.147.2.515.]
18. Shirani-Bidabadi L, Mahmoudi M, Sarabi S, Zolfaghari- Baghbaderani A, Nilforoushzadeh MA, Abdoli H. Moatar The effectiveness of mix extracts of Thyme, yarrow, and propolis on cutaneous Leishmaniasis: a comparative study in an animal model (Balb/c). J Tehran Univ Med Sci. 2009; 66(11): 785-790 [Persian].
19. Barati M, Sharifi I, Sharififar F. Antileishmanial activity of Artimisia aucheri, Ferula asa foetid and Gossypium hirsutum extracts on Leishmania Major Promastigotes in vitro. JAUMS. 2010: 8(3): 166-172. [Persian]
20. de Moraes Alves MM, Brito LM, Souza AC, Queiroz BC, de Carvalho TP, Batista JF, et al. Gallic and ellagic acids: two natural immunomodulator compounds solve infection of macrophages by Leishmania major. Naunyn-Schmiedeberg's archives of pharmacology. 2017; 390(9): 893-903.
https://doi.org/10.1007/s00210-017-1387-y [DOI:10.1007/s00210-017-1387-y.]
21. Sadeghi-Nejad B, Saki j, Azish M. Effect of Allium cepa and Ixora brachiata Root Extract on Leishmania major Promastigotes. Jundishapur J Nat Pharm Prod. 2014; 9(2):1-3.
https://doi.org/10.17795/jjnpp-15442 [DOI:10.17795/jjnpp-15442.]
22. Shemshadi B, Ranjbar-Bahadory Sh, Ahmadi H. Effect of Caparis spinosa root extract on promastigotes and amastigotes of Leishmania major. Jof Paramedical Sci. 2015; 6(1):18-23. http://dx.doi.org/10.22037/jps.v6i1.8053.
23. Yosefi R, Ghaffarifar F, Dalimi Asl A. The Effect of Alkanna tincturia and Peganum harmala Extracts on Leishmania major (MRHO/IR/75/ER) in vitro. Iranian J Parasitol. 2009; 4(1): 40-47.
24. Esmaeili J, Mohebali M, Edrissian GH, Rezayat SM, Ghazi-Khansari M, Charehdar S. Evaluation of Miltefosine against Leishmania major(MRHO/IR/75/ER) in vitro and in vivo Studies. Acta Medica Iranica. 2008; 46(3):191-196.
25. Kheirandish F, Delfan B, Mahmoudvand H, Moradi N, Ezatpour B, Ebrahimzadeh F, Rashidipour M. Antileishmanial, antioxidant, and cytotoxic activities of Quercus infectoria Olivier extract. Biomedicine & Pharmacotherapy. 2016; 82: 208-15.
https://doi.org/10.1016/j.biopha.2016.04.040 [DOI:10.1016/j.biopha.2016.04.040.]
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.