Volume 12, Issue 5 (Sep-Oct 2018)                   mljgoums 2018, 12(5): 10-15 | Back to browse issues page

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Yazdi M, Bouzari M, Ghaemi E A. Detection of fim, pap, sfa and afa Adhesin-Encoding Operons in Escherichia coli Strains Isolated from Urinary Tract Infections. mljgoums. 2018; 12 (5) :10-15
URL: http://mlj.goums.ac.ir/article-1-1114-en.html
1- Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
2- Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran , bouzari@sci.ui.ac.ir
3- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Abstract:   (6909 Views)
             Background and objectives: Urinary tract infections (UTIs) are one of the most common infectious diseases caused by bacteria. The primary etiologic agent of UTIs is Escherichia coli. Uropathogenic E.coli (UPEC) strains have a number of specific virulence factors, which can worsen UTIs. This study was performed to detect fim, pap, sfa and afa genes among E.coli strains isolated from UTIs.
             Methods: A total of 100 E. coli isolates from patients with UTI was collected between June and December 2015 from Mosavi and Sayyad Shirazi hospitals in Gorgan, Iran. All bacterial isolates were identified via standard biochemical testing and Gram straining. Presence of the genes was assessed by polymerase chain reaction.
             Results: The frequency of the fim, pap, sfa and afa genes was 100%, 79%, 69% and 8%, respectively. All isolates contained at least one virulence gene. Prevalence of multiple adhesion genes was 6% for all genes and 65% for three genes (fim, pap and sfa) together. In addition, the frequency of the fim gene was significantly higher than that of the other genes (P<0.0001).
             Conclusion: The results of this study indicate the high prevalence of virulence factors that can enhance pathogenicity of E. coli. Therefore, these factors could be used as diagnostic markers or vaccine targets.
             Keywords: Virulence factors, Urinary tract infection, Uropathogenic Escherichia coli.
Full-Text [PDF 561 kb]   (961 Downloads)    
Type of Study: Review Article |
Received: 2018/08/13 | Accepted: 2018/08/13 | Published: 2018/08/13 | ePublished: 2018/08/13

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