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mljgoums 2020, 14(4): 13-19 |
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Ebrahimi A, Rabiaee S, Lotfalian S, Habibian S. Effect of Clove Essential Oil (Syzygium aromaticum) on Some Virulence Factors of Staphylococcus aureus. mljgoums 2020; 14 (4) :13-19
URL: http://mlj.goums.ac.ir/article-1-1112-en.html
URL: http://mlj.goums.ac.ir/article-1-1112-en.html
1- Department of Pathobiology, Veterinary College, , a_kahrizsangi@yahoo.com
2- Department of Pathobiology, Veterinary College,
3- Department of Pharmacology, Veterinary College,
2- Department of Pathobiology, Veterinary College,
3- Department of Pharmacology, Veterinary College,
Abstract: (3349 Views)
ABSTRACT
Background and objectives: Clove (Syzygium aromaticum) essential oil is a food additive with proven antimicrobial and antioxidant properties. Thus, it may be a good candidate for controlling foodborne pathogens, such as Staphylococcus aureus. The aim of the present study was to evaluate effects of sub–minimum inhibitory concentrations (MICs) of clove oil on some virulence factors of S. aureus.
Methods: The standard strain and 12 field isolates of S. aureus were obtained from our microbial collections. The broth tube dilution method was used to determine the MIC of clove oil against the isolates. Sterile 96-well flat bottom polystyrene microtiter plates were used for planktonic growth and biofilm formation assays. Slide coagulase test was used for assaying effect of clove oil on clumping factor production. Production of α- and β-hemolysins was assessed by culture on 5% bovine blood agar.
Results: The results showed that sub-MIC concentrations of clove oil inhibited α- and β-hemolysins and biofilm production and planktonic growth of the examined isolates. However, clumping factor was not affected by sub-MIC concentrations of clove oil.
Conclusion: Our results indicate the favorable inhibitory effects of sub-MIC concentrations of clove oil against growth and biofilm and hemolysins production of S. aureus isolates.
Background and objectives: Clove (Syzygium aromaticum) essential oil is a food additive with proven antimicrobial and antioxidant properties. Thus, it may be a good candidate for controlling foodborne pathogens, such as Staphylococcus aureus. The aim of the present study was to evaluate effects of sub–minimum inhibitory concentrations (MICs) of clove oil on some virulence factors of S. aureus.
Methods: The standard strain and 12 field isolates of S. aureus were obtained from our microbial collections. The broth tube dilution method was used to determine the MIC of clove oil against the isolates. Sterile 96-well flat bottom polystyrene microtiter plates were used for planktonic growth and biofilm formation assays. Slide coagulase test was used for assaying effect of clove oil on clumping factor production. Production of α- and β-hemolysins was assessed by culture on 5% bovine blood agar.
Results: The results showed that sub-MIC concentrations of clove oil inhibited α- and β-hemolysins and biofilm production and planktonic growth of the examined isolates. However, clumping factor was not affected by sub-MIC concentrations of clove oil.
Conclusion: Our results indicate the favorable inhibitory effects of sub-MIC concentrations of clove oil against growth and biofilm and hemolysins production of S. aureus isolates.
Research Article: Original Paper |
Subject:
Microbiology
Received: 2018/08/12 | Accepted: 2020/04/13 | Published: 2020/06/30 | ePublished: 2020/06/30
Received: 2018/08/12 | Accepted: 2020/04/13 | Published: 2020/06/30 | ePublished: 2020/06/30
References
1. Priyanka B, Patil R-K, Dwarakanath S. A review on detection methods used for foodborne pathogens. Indian J Med Res. 2016; 144(3): 327-338. doi: 10.4103/0971-5916.198677. [DOI:10.4103/0971-5916.198677] [PubMed] [Google Scholar]
2. World Health Organization"WHO". Food safety and foodborne illness. Geneva: Switzerland. Fact sheet 237. 2002. [Google Scholar]
3. Bautista-Baños S, Hernandez-Lauzardo A-N, Velazquez-Del Valle M-G, Hernández-López M, Barka E-A, Bosquez-Molina E, et al. Chitosan as a potential natural compound to control pre and postharvest diseases of horticultural commodities. Crop Protection. 2016; 25(2): 108-118. DOI: 10.1016/j.cropro.2005.03.010. [DOI:10.1016/j.cropro.2005.03.010] [Google Scholar]
4. Matan N, Rimkeeree H, Mawson A-J, Chompreeda P, Haruthaithanasan V, Parker M. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. International journal of Food Microbiology. 2006; 107(2): 180-185. [DOI:10.1016/j.ijfoodmicro.2005.07.007] [PubMed] [Google Scholar]
5. Karapinar M, Aktug S-E. Inhibition of foodborne pathogens by thymol, eugenol, menthol and anethole. International Journal of Food Microbiology.1987; 4(2): 161-166. [DOI:10.1016/0168-1605(87)90023-7] [Google Scholar]
6. Majerczyk C-D, Sadykov M-R, Luong T-T, Lee C, Somerville G-A, Sonenshein A-L. Staphylococcus aureus CodY negatively regulates virulence gene expression. Journal of bacteriology. 2008; 190: 2257-2265. DOI:10.1128/JB.01545-07. [DOI:10.1128/JB.01545-07] [PubMed] [Google Scholar]
7. Ebrahimi A, Akhavan Taheri M. Characteristics of staphylococci isolated from clinical and subclinical mastitis cows in Shahrekord, Iran. Iranian journal of veterinary research. 2008; 10: 273-277. [Google Scholar]
8. Zell C, Resch M, Rosenstein R, Albrecht T, Hertel C, Götz F. Characterization of toxin production of coagulase-negative staphylococci isolated from food and starter cultures. International journal of food microbiology. 2008; 127(3): 246-51. [DOI:10.1016/j.ijfoodmicro.2008.07.016] [PubMed] [Google Scholar]
9. Qiu J, Wang D, Xiang H, Feng H, Jiang Y, Xia L, et al. Subinhibitory concentrations of thymol reduce enterotoxins A and B and α-hemolysin production in Staphylococcus aureus isolates. PLoS one. 2010; 5: e9736. [DOI:10.1371/journal.pone.0009736] [PubMed] [Google Scholar]
10. Otto M. Basis of virulence in community-associated methicillin-resistant Staphylococcus aureus. Annual review of microbiology. 2010; 64: 143-162. [DOI:10.1146/annurev.micro.112408.134309] [PubMed] [Google Scholar]
11. Hall-Stoodley L, Costerton J-W, Stoodley P. Bacterial biofilms: from the natural environment to infectious diseases. Nature reviews microbiology. 2004; 2(2): 95-108. [DOI:10.1038/nrmicro821] [PubMed] [Google Scholar]
12. Bhakdi S, Tranum-Jensen J. Alpha-toxin of Staphylococcus aureus. Microbiology and Molecular Biology Reviews. 1991; 55(4):733-751. [DOI:10.1128/MMBR.55.4.733-751.1991] [Google Scholar]
13. Huseby M, Shi K, Brown C-K, Digre J, Mengistu F, Seo K-S, et al. Structure and biological activities of beta toxin from Staphylococcus aureus. Journal of bacteriology. 2007; 189(23): 8719-8726. DOI: 10.1128/JB.00741-07. [DOI:10.1128/JB.00741-07] [PubMed] [Google Scholar]
14. Smith-Palmer A, Stewart J, Fyfe L. Influence of subinhibitory concentrations of plant essential oils on the production of enterotoxins A and B and α-toxin by Staphylococcus aureus. J Med Microbiol. 2004; 53(Pt 10): 1023-1027. doi: 10.1099/jmm.0.45567-0. [DOI:10.1099/jmm.0.45567-0] [PubMed] [Google Scholar]
15. Qiu J, Feng H, Lu J, Xiang H, Wang D, Dong J, Deng X, et al. Eugenol reduces the expression of virulence-related exoproteins in Staphylococcus aureus. Applied and environmental microbiology. 2010; 76: 5846-5851. DOI: 10.1128/AEM.00704-10. [DOI:10.1128/AEM.00704-10] [PubMed] [Google Scholar]
16. Yadav M-K, Chae S-W, Im G-J, Chung J-W, Song J-J. Eugenol: a phyto-compound effective against methicillin-resistant and methicillin-sensitive Staphylococcus aureus clinical strain biofilms. PLoS One. 2015; 10: e0119564. [DOI:10.1371/journal.pone.0119564] [PubMed] [Google Scholar]
17. Husain F-M, Ahmad I, Asif M, Tahseen Q. Influence of clove oil on certain quorum-sensing-regulated functions and biofilm of Pseudomonas aeruginosa and Aeromonas hydrophila. Journal of biosciences. 2013; 38(5): 835-844. [DOI:10.1007/s12038-013-9385-9] [PubMed] [Google Scholar]
18. Khan M-S-A, Zahin M, Hasan S, Husain F-M, Ahmad I.Inhibition of quorum sensing regulated bacterial functions by plant essential oils with special reference to clove oil . Letters in applied microbiology. 2009; 49: 354-360. DOI: 10.1111/j.1472-765X.2009.02666.x. [DOI:10.1111/j.1472-765X.2009.02666.x] [PubMed] [Google Scholar]
19. Adams R-P. Identification of essential oil components by gas chromatography/mass spectroscopy. Journal of the American Society for Mass Spectrometry. 1997; 6: 671-672. [DOI:10.1016/S1044-0305(97)00026-3] [Google Scholar]
20. Olajuyigbe O-O, Afolayan A-J. In vitro antibacterial and time-kill evaluation of the Erythrina caffra Thunb. extract against bacteria associated with diarrhoea. The Scientific World Journal. 2012. [DOI:10.1100/2012/738314] [PubMed] [Google Scholar]
21. Ebrahimi A, Ghasemi M, Ghasemi B. Some virulence factors of staphylococci isolated from wound and skin infections in Shahrekord, IR Iran. Jundishapur J Microbiol. 2014; 7(4): e9225. doi: 10.5812/jjm.9225. [DOI:10.5812/jjm.9225] [PubMed] [Google Scholar]
22. Houari A, Di Martino P. Effect of chlorhexidine and benzalkonium chloride on bacterial biofilm formation. Letters in applied microbiology. 2007; 45(6): 652-6. [DOI:10.1111/j.1472-765X.2007.02249.x] [PubMed] [Google Scholar]
23. Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I, et al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS. 2007; 115: 891-899. [DOI:10.1111/j.1600-0463.2007.apm_630.x] [PubMed] [Google Scholar]
24. Moon S-E, Kim H-Y, Cha J-D. Synergistic effect between clove oil and its major compounds and antibiotics against oral bacteria. Arch Oral Biol. 2011; 56(9): 907-16. doi: 10.1016/j.archoralbio.2011.02.005. [DOI:10.1016/j.archoralbio.2011.02.005] [PubMed] [Google Scholar]
25. Selestino Neta M-C, Vittorazzi C, Guimarães A-C, Martins J-D-L, Fronza M, Endringer D-C, et al. Effects of β-caryophyllene and Murraya paniculata essential oil in the murine hepatoma cells and in the bacteria and fungi 24-h time-kill curve studies. Pharmaceutical biology. 2017; 55(1): 190-7. [DOI:10.1080/13880209.2016.1254251] [PubMed] [Google Scholar]
26. Kalemba D-A-A-K, Kunicka A. Antibacterial and antifungal properties of essential oils. Current medicinal chemistry. 2003; 10(10): 813-829. [DOI:10.2174/0929867033457719] [PubMed] [Google Scholar]
27. Espina L, Pagán R, López D, García-Gonzalo D. Individual constituents from essential oils inhibit biofilm mass production by multi-drug resistant Staphylococcus aureus. Molecules. 2015; 20(6): 11357-72. doi: 10.3390/molecules200611357. [DOI:10.3390/molecules200611357] [PubMed] [Google Scholar]
28. Hammer K-A, Riley T-V, Carson C-F. Effects of tea tree oil on Staphylococcus aureus virulence factors: A report for the rural industries research and development corporation. Rural Industries Research and Development Corporation. 2005; Barton, A.C.T : Rural Industries Research. [Google Scholar]
29. Gemmell C-G, Ford C-W. Virulence factor expression by Gram-positive cocci exposed to subinhibitory concentrations of linezolid. Journal of Antimicrobial Chemotherapy. 2002; 50(5): 665-672. [DOI:10.1093/jac/dkf192] [PubMed] [Google Scholar]
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