Volume 13, Issue 5 (Sep-Oct 2019)                   mljgoums 2019, 13(5): 26-31 | Back to browse issues page

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Kelishadi M, Hashemi P, Ashrafi G, Behnampour N, Tabarraei A. Presence of GB Virus C in Whole-Blood Derivatives: A Pilot Study. mljgoums. 2019; 13 (5) :26-31
URL: http://mlj.goums.ac.ir/article-1-1106-en.html
1- Laboratory Sciences Research Center, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran- Department of Virology, Pasteur Institute of Iran, Tehran, Iran
2- Infectious Diseases Research Center, Department of Microbiology، Golestan University of Medical Sciences, Gorgan, Iran
3- Kingston University London, Cancer Theme, School of Life Science, Pharmacy and Chemistry, SEC Faculty, Kingston upon Thames, KT12EE, London, UK
4- Department of Statistic, Paramedical School, Golestan University of Medical Sciences, Gorgan, Iran
5- Infectious diseases research centre, Department of microbiology, Golestan University of , alijant@yahoo.com
Abstract:   (324 Views)
ABSTRACT
              Background and Objectives: Red blood cell (RBC) transfusion is necessary for the prevention and treatment of a variety of life-threatening injuries and diseases. However, viral contamination of these products is a great threat to recipients. Screening donors for GB virus C by nucleic acid testing is not routinely implemented worldwide. The aim of the present study was to evaluate prevalence of GBV-C RNA in whole blood/red cell components.
              Methods: In this cross sectional pilot study, we collected 153 units of packed RBCs from blood banks of two public hospitals in Gorgan (northeast of Iran), between October and November 2014. The samples were screened for the presence of GBV-C RNA in plasma by nested RT-PCR using specific primers targeting highly conserved regions of 5' UTR of GBV-C. Data were analyzed using SPSS software (version 18).
              Results: Overall, 48 (31.37%) whole blood or red cell components were positive for GBV-C viremia. The GBV-C RNA was detected in 31/88 citrate phosphate dextrose-adenine 1 (CPDA1) RBC, 16/50 washed RBC and 1/13 reduced-leukocyte RBC. However, whole blood CPDA1 was negative for GBV-C viremia. Direct sequencing of PCR products confirmed GBV-C contamination.
              Conclusions: Transmission of GBV-C infection was observed in blood products. Thus, efforts should be made to develop new strategies for assuring blood transfusion safety.
              Keywords: Molecular testing, Epidemiology, Transfusion-transmissible infections, GB Virus C.
Full-Text [PDF 724 kb]   (49 Downloads)    
Subject: Microbiology
Received: 2018/07/17 | Accepted: 2019/06/1 | Published: 2019/09/2 | ePublished: 2019/09/2

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