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Charmi far B, Mahdavi S. Frequency of Adherence Genes cna, fnbA and fnbB in Staphylococcus aureus Isolates from Traditional Cheese. mljgoums 2017; 11 (5) :11-15
URL: http://mlj.goums.ac.ir/article-1-1014-en.html
URL: http://mlj.goums.ac.ir/article-1-1014-en.html
1- Department of Microbiology, Urmia Branch, Islamic Azad University, Urmia, Iran
2- Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran ,S.mahdavi@iau-maragheh.ac.ir
2- Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran ,
Abstract: (8265 Views)
ABSTRACT
Background and Objectives: Several virulence factors are involved in the pathogenesis of Staphylococcus aureus. Surface proteins such as collagen binding proteins (Cna) and fibronectin-binding proteins (FnBP) are important factors in adhesion and invasion of S. aureus. The aim of this study was to evaluate the frequency of adherence genes cna, fnbA and fnbB S. aureus isolates from traditional cheese.
Methods: All 22 isolates tested were identified as S. aureus. The isolates were tested for the presence of adherence genes cna, fnbA and fnbB using specific primers in polymerase chain reaction assay.
Results: Six isolates (27.27%) were positive for the can gene. Of the 22 isolates studied, one isolate was positive for fnbA and one was positive for the fnbB. Co-presence of the genes examined was not observed in any of the isolates.
Conclusion: The results indicate the weak biofilm formation ability of the S. aureus isolates from traditional cheese.
Keywords: Staphylococcus aureus, Biofilm, Genes, Cheese.
Background and Objectives: Several virulence factors are involved in the pathogenesis of Staphylococcus aureus. Surface proteins such as collagen binding proteins (Cna) and fibronectin-binding proteins (FnBP) are important factors in adhesion and invasion of S. aureus. The aim of this study was to evaluate the frequency of adherence genes cna, fnbA and fnbB S. aureus isolates from traditional cheese.
Methods: All 22 isolates tested were identified as S. aureus. The isolates were tested for the presence of adherence genes cna, fnbA and fnbB using specific primers in polymerase chain reaction assay.
Results: Six isolates (27.27%) were positive for the can gene. Of the 22 isolates studied, one isolate was positive for fnbA and one was positive for the fnbB. Co-presence of the genes examined was not observed in any of the isolates.
Conclusion: The results indicate the weak biofilm formation ability of the S. aureus isolates from traditional cheese.
Keywords: Staphylococcus aureus, Biofilm, Genes, Cheese.
Research Article: Original Paper |
Received: 2017/11/15 | Accepted: 2017/11/15 | Published: 2017/11/15 | ePublished: 2017/11/15
Received: 2017/11/15 | Accepted: 2017/11/15 | Published: 2017/11/15 | ePublished: 2017/11/15
References
1. Kumar CG, Anand SK. Significance of microbial biofilms in food industry: a review. Int J Food Microbiol. 1998; 42(1-2): 9-27. [DOI:10.1016/S0168-1605(98)00060-9]
2. Wanasinghe DD. In vitro adherence of Staphylococcus aureus to bovine mammary gland epithelial cells. Acta Vet Scand. 1981; 22: 99-108. PMID: 7258035.
3. Gudding R, McDonald JS, Cheville NF. Pathogenesis of Staphylococcus aureus mastitis: bacteriologic, histologic, and ultrastructural pathologic findings. Am J Vet Res. 1984; 45: 2525-31.
4. Foster TJ, Hook M. Surface protein adhesins of Staphylococcus aureus. Trends Microbiol. 1998; 6(12): 484-8. PMID:10036727. [DOI:10.1016/S0966-842X(98)01400-0]
5. Hauck CR, Ohlsen K. Sticky connections: extracellular matrix protein recognition and integrin-mediated cellular invasion by Staphylococcus aureus. Curr Opin Microbiol. 2006; 9(1): 5-11. [DOI:10.1016/j.mib.2005.12.002]
6. Jett BD, Gilmore MS. Internalization of Staphylococcus aureus by human corneal epithelial cells: role of bacterial fibronectin binding protein and host cell factors. Infect Immun. 2002;70:4697-700. doi: 10.1128/IAI.70.8.4697-4700.2002. [DOI:10.1128/IAI.70.8.4697-4700.2002]
7. Smeltzer MS, Gillaspy AF. Molecular pathogenesis of staphylococcal osteomyelitis. Poultry Sci. 2000; 79(7): 1042-49. [DOI:10.1093/ps/79.7.1042]
8. Cheung AL, Fischetti VA. The role of fibrinogen in mediating staphylococcal adherence to fibers. J Surg Res. 1991; 50(2): 150-5. [DOI:10.1016/0022-4804(91)90239-I]
9. Patti JM, Bremell T, Krajewska-Pietrasik D, Abdelnour A, Tarkowski A, Ryden C, et al. The Staphylococcus aureus collagen adhesin is a virulence determinant in experimental septic arthritis. Infect Immun. 1994; 62(1): 152-61.
10. Lammers A, Nuijten PJ, Smith HE. The fibronectin binding proteins of Staphylococcus aureus are required for adhesion to and invasion of bovine mammary gland cells. Fems Microbiol Lett. 1999; 180(1): 103-9. [DOI:10.1111/j.1574-6968.1999.tb08783.x]
11. Atashpaz S, Khani S, Barzegari A, Barar J, Vahed SZ, Azarbaijani R, et al. A robust universal method for extraction of genomic DNA from bacterial species. Mikrobiologiia. 2010; 79(4): 562-6. [DOI:10.1134/S0026261710040168]
12. Brakstad OG, Aasbakk K, Maeland JA. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J Clin Microbiol. 1992; 30(7): 1654-60.
13. Arciola CR, Campoccia D, Gamberini S, Baldassarri L, Montanaro L. Prevalence of cna, fnbA and fnbB adhesin genes among Staphylococcus aureus isolates from orthopedic infections associated to different types of implant. Fems Microbiol Lett. 2005; 246(1): 81-6. [DOI:10.1016/j.femsle.2005.03.035]
14. Montanaro L, Arciola CR, Baldassarri L, Borsetti E. Presence and expression of collagen adhesin gene (cna) and slime production in Staphylococcus aureus strains from orthopaedic prosthesis infections. Biomaterials. 1999; 20(20): 1945-49. [DOI:10.1016/S0142-9612(99)00099-X]
15. Montanaro L, Arciola CR, Borsetti E, Collamati S, Baldassarri L, Montanaro L. Detection of fibronectin-binding protein genes in staphylococcal strains from peri-prosthesis infections. New Microbiol. 1999; 22(4): 331-6.
16. Aarnisalo K, Lundén J, Korkeala H, Wirtanen G. Susceptibility of Listeria monocytogenes strains to disinfectants and chlorinated alkaline cleaners at cold temperatures. LWT - Food Science and Technology. 2007; 40(6): 1041-8. [DOI:10.1016/j.lwt.2006.07.009]
17. Melo PdC, Ferreira LM, Nader Filho A, Zafalon LF, Vicente HIG, Souza VD. Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis. Braz J Microbiol. 2013; 44(1): 119-24. doi: 10.1590/S1517-83822013005000031. [DOI:10.1590/S1517-83822013005000031]
18. Gotz F. Staphylococcus and biofilms. Mol Microbiol. 2002; 43(6): 1367-78. [DOI:10.1046/j.1365-2958.2002.02827.x]
19. Hudson MC, Ramp WK, Frankenburg KP. Staphylococcus aureus adhesion to bone matrix and bone-associated biomaterials. Fems Microbiol Lett. 1999; 173(2): 279-84. [DOI:10.1111/j.1574-6968.1999.tb13514.x]
20. Smeltzer MS, Gillaspy AF, Pratt FL, Thames MD, Iandolo JJ. Prevalence and chromosomal map location of Staphylococcus aureus adhesin genes. Gene. 1997; 196(1-1): 249-59.
21. Nashev D, Toshkova K, Salasia SI, Hassan AA, Lammler C, Zschock M. Distribution of virulence genes of Staphylococcus aureus isolated from stable nasal carriers. Fems Microbiol Lett. 2004; 233: 45-52. DOI:10.1016/j.femsle.2004.01.032. [DOI:10.1016/j.femsle.2004.01.032]
22. Reinoso EB, El-Sayed A, Lammler C, Bogni C, Zschock M. Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina. Microbiol Res. 2008; 163(3): 314-22. DOI:10.1016/j.micres.2006.05.013. [DOI:10.1016/j.micres.2006.05.013]
23. Elasri MO, Thomas JR, Skinner RA, Blevins JS, Beenken KE, Nelson CL, et al. Staphylococcus aureus collagen adhesion contributes to the pathogenesis of osteomyelitis. Bone. 2002; 30(1): 275-80. [DOI:10.1016/S8756-3282(01)00632-9]
24. Dastmalchi saei H, Aghdasi S, Mohamad zadeh H. Frequency analysis of adhesin genes cna, fnbA and fnbB in Staphylococcus aureus isolates from sheep mastitis. Iran Vet J. 2013; 9(1): 19-29. [Persian]
25. Thomas MG, Peacock S, Daenke S, Berendt AR. Adhesion of Staphylococcus aureus to collagen is not a major virulence determinant for septic arthritis, osteomyelitis, or endocarditis. J Infect Dis. 1999; 179(1): 291-3. [DOI:10.1086/314576]
26. Lammers A, Nuijten PJ, Kruijt E, Stockhofe-Zurwieden N, Vecht U, Smith HE, et al. Cell tropism of Staphylococcus aureus in bovine mammary gland cell cultures. Vet Microbiol. 1999; 67(2): 77-89. [DOI:10.1016/S0378-1135(99)00018-8]
27. Dziewanowska K, Patti JM, Deobald CF, Bayles KW, Trumble WR, Bohach GA. Fibronectin binding protein and host cell tyrosine kinase are required for internalization of Staphylococcus aureus by epithelial cells. Infect Immun. 1999; 67(9): 4673-78.
28. Duran N, Dogramaci Y, Ozer B, Demir C, Kalaci A. Detection of adhesin genes and slime production among Staphylococci in orthopaedic surgical wounds. Afr J Microbiol Res. 2010; 4(9): 708-15.
29. Hensen SM, Pavicic MJ, Lohuis JA, Poutrel B. Use of bovine primary mammary epithelial cells for the comparison of adherence and invasion ability of Staphylococcus aureus strains. J Dairy Sci. 2000; 83(3): 418-29. [DOI:10.3168/jds.S0022-0302(00)74898-3]
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