Golestan University of Medical Sciences Medical Laboratory Journal 2538-4449 16 4 2022 7 1 Adverse Effects of Hypothyroidism on Fertility and Pregnancy: A Mini Review 1 9 EN Akhtar Seifi Department of Pharmacology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Negarsadat Taheri Department of Gynecology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Hanieh Kia Department of Clinical Research, Clinical Science Institute, Santa Monica, CA 90404, USA Hady Reza Mansourian Department of Emergency, Hakim Jorjani Hospital, Gorgan, Iran Azad Reza Mansourian Metabolic Disorders Research Center, Department of Biochemistry, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran The prevalence of hypothyroidism is much higher among women than in men. Hypothyroidism is also one of the most prevalent thyroid disorders among women of reproductive age. The disease exerts its effect on female sex hormones by manipulating the production of luteinizing hormone and follicle-stimulating hormone that are crucial for the production of estrogen by the ovaries. Various studies demonstrated the adverse effect of overt hypothyroidism on ovulation, menstrual cycle, and fertility. This review surveys the adverse effects of hypothyroidism on fertility and pregnancy.  

Golestan University of Medical Sciences Medical Laboratory Journal 2538-4449 16 4 2022 7 1 Utility of Fine Needle Aspiration Cytology in the Evaluation of Palpable Cervical Swelling: A Study of 100 Cases at a Tertiary Care Hospital 10 14 EN Usha Patel Department of Biochemistry, AMC MET Medical College, Ahmedabad, India Nanda Jagrit Department of Pathology, AMC MET Medical College, Ahmedabad, India Shubham Panchal Department of Pathology, AMC MET Medical College, Ahmedabad, India Ankita Kacha Department of Pathology, AMC MET Medical College, Ahmedabad, India Rujuta Ravat Department of Pathology, AMC MET Medical College, Ahmedabad, India Background and objectives: Lesions of the head and neck region are routinely encountered by clinicians, in patients across all age groups. Diagnoses range from reactive inflammatory conditions to malignancies. Cancer is among the leading causes of death in India. Head and neck cancers account for 23% of all cancer incidents in males and 6% of all incidents in females in India,. Fine needle aspiration cytology (FNAC) of cervical masses is an easy, effective, and relatively inexpensive technique. The aim of this study was to determine occurrence of various head and neck swellings and classify them under various categories. Methods: The present study included 100 cases of palpable cervical swellings who had been referred to the AMC MET Medical College, Ahmedabad (India) from June 2018 to October 2020. Results: The majority of cases were lymph node swellings (57%), followed by thyroid swellings (23%), salivary gland swellings (12%), and soft tissue swellings (8%). Among all cervical region swellings, tuberculous lymphadenitis and thyroid lesions were the most prevalent.  Conclusion: The findings suggest that lymph node swellings are most commonly observed in the cervical area, and that the majority of them are inflammatory, requiring medical treatment rather than surgery. Moreover, FNAC is a straightforward, quick, and cost-effective way to distinguish between non-neoplastic and metastatic malignant lesions, which could help timely surgical interventions.  

Golestan University of Medical Sciences Medical Laboratory Journal 2538-4449 16 4 2022 7 1 Effects of Bacterial Cellulose Nanofiber on Lead Concentration in Kidney and Liver Tissues of Wistar Rats 15 19 EN Nahid Valipoor Department of Environmental Sciences, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Iran. Somayeh Namroodi Department of Environmental Sciences, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran Shohreh Taziki Ischemic Disorders Research Center ٫ Golestan University of Medical Sciences, Gorgan, Iran Hassan Rezaei Department of Environmental Sciences, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Background and objectives: Lead (Pb) is among the most toxic pollutants that affect health of both humans and animals. Finding a way to prevent Pb accumulation in animals’ bodies seems necessary. Bacterial cellulose nanofiber (BCNF) can remove heavy metals from aqueous solutions. This study investigates effects of oral consumption of BCNF, as a chelator, on Pb concentration in the kidney and liver tissues of rats. Methods: Sixteen Wistar rats (aged 6-8 weeks) were divided into four groups: 1. control, 2. fed with Pb, 3. fed with Pb (50 μg/g) and BCNF (16 μg/g) simultaneously, and 4. fed with Pb and BCNF with 4 hours interval. The rats were euthanized, and the kidney and liver tissues were separated. After acidic digestion of the tissue samples, Pb concentration was measured by atomic absorption spectrometry. Results: The mean concentration of Pb in the kidney and liver tissues of rats fed with Pb and BCNF were significantly lower than that of rats fed only with Pb. In addition, the mean Pb concentration in rats of group 3 was lower than that of group 4. Conclusion: The results of this study showed the favorable effects of BCNF on prevention of Pb accumulation in the kidney and liver tissues of rats. Moreover, removal of Pb may be related to binding of BCNF with Pb in the gut or blood. More studies are necessary to determine the exact mechanisms through which BCNF can reduce Pb accumulation.  

Golestan University of Medical Sciences Medical Laboratory Journal 2538-4449 16 4 2022 7 1 Detoxification of AFB1 by Yeasts Isolates from Kefir and Traditional Kefir-Like Products 20 25 EN Abdoljalil Eiri Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Hami kaboosi Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Farhad Niknejad Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran and Department of Clinical Laboratory Sciences, Faculty of Para Medicine, Golestan University of Medical Sciences, Gorgan, Iran Abdollah Ardebili Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran and Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Hamid Reza joshaghani Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran and Department of Clinical Laboratory Sciences, Faculty of Para Medicine, Golestan University of Medical Sciences, Gorgan, Iran Background and objectives: Aflatoxin B1 (AFB1) is the most toxic aflatoxin produced by a large number of Aspergillus species. Successful detoxification of this toxin is an important attempt to improve community health. The aim of this study was to evaluate reducing effects of yeasts isolates from kefir and traditional kefir-like fermented beverages on AFB1 in a broth medium. Methods: Polymerase chain reaction-sequencing was carried out to identify the yeast isolates from kefir and kefir-like beverages. Effects of the isolates on AFB1 adsorption and biotransformation in peptone dexterose broth medium were evaluated by using high performance liquid chromatography. Results: Saccharomyces cerevisiae and Kluyveromyces marxianus were isolated from kefir and kefir-like beverages and resulted in 46% and 53% AFB1 adsorption, respectively. The isolates 27Y and 2Y caused 7% toxin biotransformation, while 10% toxin biotransformation was achieved by the isolate 18Y.  Conclusion: Our results indicate that the yeast isolates from kefir and traditional kefir-like products can bind to and detoxify AFB1, thereby reducing its harmful effects.

Golestan University of Medical Sciences Medical Laboratory Journal 2538-4449 16 4 2022 7 1 In Vitro Protoscolicidal Activity of Pomegranate (Punica Granatum) Rind and Barberry (Berberis Vulgaris) Alcoholic Extracts against Hydatid Cysts Caused by Echinococcus granulosus 26 31 EN Shahab Shiri Hamedani Student Research Committee, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran Mohsen Mansouri Department of Persian Traditional Medicine, AJA University of Medical Sciences, Tehran, Iran Sina Shiri Hamedani Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran Parham Tadayon Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran Peyman Aslani Department of Parasitology and Mycology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran Mohammad Mohsen Homayouni Department of Parasitology and Mycology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran Background and objectives: Echinococcosis is a global cosmopolitan zoonotic disease and a major veterinary and public health issue. In humans, echinococcosis usually develops following close contact with infected dogs or ingestion of the parasite eggs. Until now, no effective vaccine has been commercially developed, and treatment is only focused on controlling hydatidosis. This study was conducted to evaluate the protoscolicidal activity of alcoholic extracts of pomegranate rind and barberry. Methods: The alcoholic extracts of pomegranate rind and barberry were prepared by mixing 330 g of powdered plants with 1,000 ml of 70% ethanol. A concentrate of viable protoscolices (PCSs) was obtained from hydatid cysts found in the lungs and liver of sheep. Next, PCSs were treated with four different concentrations (5, 10, 20, 30, and mg/ml) of each extract for 10, 20, 30, and 60 minutes. The eosin exclusion test was performed to assess viability of the PCSs. Results: The mortality rate caused by treatment with the extracts ranged between 25% and 100%. Complete inactivation of PCSs was achieved after 60 minutes of exposure to 15 mg/ml of the pomegranate rind extract and 30 mg/ml of the barberry extract. Conclusion: Given their favorable anti-PCSs activity, combination of conventional synthetic albendazole with the alcoholic extracts of pomegranate rind and barberry might induce higher anti-PCS activity with lower side effects. It is recommended to evaluate the anti-PCSs activities of the pomegranate rind and barberry alcoholic extracts in vivo and ex vivo.

Golestan University of Medical Sciences Medical Laboratory Journal 2538-4449 16 4 2022 7 1 Evaluation of Gastroprotective Activity of Licorice and Turmeric Rhizome Aqueous Extract against Ethanol-Induced Gastric Injury in Male Wistar Rats 32 38 EN Mohammad Panji Research Center for Life & Health Sciences & Biotechnology of the Police, Directorate of Health, Rescue & Treatment, Police Headquarter, Tehran, Iran Marzieh Ghafouri Department of Cell and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran Background and objectives: Gastric ulcers occur when the mucous layer covering the gastric wall is damaged. Curcuma and licorice are famous ancient herbs that have been widely used as food additives and medicinal herbs. The present study aimed to investigate gastroprotective activity of Curcuma and licorice extract (CLE) against ethanol-induced gastric ulcers in male Wistar rats. Methods: Fifteen male Wistar rats were randomly divided into five groups and fasted for 24 hours with free access to water. The rats received different doses of CLE (200 and 500 mg/kg) or omeprazole (20 mg/kg) by oral gavage. One hour after the induction of gastric ulcer via absolute ethanol administration (5 ml/kg), the rats were sacrificed, and the stomach was opened along the greater curvature. Ulcerative lesions were observed and counted. Total juice acidity was evaluated. Finally, mRNA level of Bax and COX-2 was measured. Results: The oral administration of CLE (200 and 500 mg/kg) and omeprazole (positive control, 20 mg/kg) to rats remarkably attenuated gastric acidity and the number of ethanol-induced gastric lesions. Further examination of gastric mucosal homogenate revealed significant downregulation of Bax and COX-2 in the CLE-treated groups compared with the lesion control group.  Conclusion: The findings of this study confirm the gastroprotective activity of CLE against ethanol-induced gastric injury.

Golestan University of Medical Sciences Medical Laboratory Journal 2538-4449 16 4 2022 7 1 Effects of Resveratrol Supplementation and Exercise on Apoptosis, Lipid Profile, and Expression of Farnesoid X Receptor, Liver X Receptor and Sirtuin 1 Genes in the Liver of Type 1 Diabetic Rats 39 46 EN Ali Nouri Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran Parvin Farzanegi Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran Mohammad Ali Azarbayjani Department of Exercise Physiology, Islamic Azad University, Central Tehran Branch Background and objectives: Diabetes mellitus is the most common metabolic disorder in the world. Here, we evaluated effects of resveratrol supplementation alone and combined with exercise on blood glucose, insulin, lipid profile, apoptosis biomarkers, and expression of farnesoid X receptor (Fxr), liver X receptor (Lxr), and sirtuin 1 (Sirt 1) genes in the liver of type 1 diabetic rats. Methods: Streptozotocin was used to induce type 1 diabetes in Wistar rats. The rats were randomly assigned into seven groups. After treatment with resveratrol alone or combined with exercise training, the animals were sacrificed and lipid profile and levels of blood glucose and insulin were measured. Hepatocyte apoptosis was assessed by measuring the level of Bax and Bcl2 proteins using enzyme-linked immunosorbent assay kits. Expression of Fxr, Lxr, and Sirt1 was evaluated using real-time polymerase chain reaction. Comparison of the mean levels of all variables between different groups was performed using one-way analysis of variance, at statistical significance level of 0.05. Results: Resveratrol significantly reduced the level of blood glucose and insulin compared with the control groups (p<0.001). It also significantly affected the lipid profile (p<0.001). Diabetes was significantly associated with decreased expression of Sirt1, Lxr, and Fxr and increased hepatocyte apoptosis. Resveratrol significantly improved the expression of all three genes (p<0.01). Overall, resveratrol supplementation combined with exercise was more effective than other methods. Conclusion: The results indicate that that combination of resveratrol therapy with exercise could be beneficial for diabetic patients. However, more studies are needed to confirm this finding.  

Golestan University of Medical Sciences Medical Laboratory Journal 2538-4449 16 4 2022 7 1 Development of a Validation Protocol for Laboratory Personnel Training in Sperm Analysis and Cryopreservation(brief report) 47 53 EN Karolyn Sassi Ogliari Hemocord Biotechnology, São Leopoldo, Brazil Monica Luiza Immig Hemocord Biotechnology, São Leopoldo, Brazil Maria Laura Halon Hemocord Biotechnology, São Leopoldo, Brazil Fabrízio Loth Hemocord Biotechnology, São Leopoldo, Brazil Luiza Boni University of Vale do Rio dos Sinos, São Leopoldo, Brazil Patrícia Grudzinski Hemocord Biotechnology, São Leopoldo, Brazil Liziane Beckenkamp Hemocord Biotechnology, São Leopoldo, Brazil Martina Fritsch Hemocord Biotechnology, São Leopoldo, Brazil Background and objectives: Semen cryopreservation is widely used in assisted reproduction techniques, and reliable semen analysis is essential to define the clinical practice. However, many parameters used for semen evaluation have high variability among technicians. Here, we describe a method of validating semen analysis prior to cryopreservation, comparing each operator’s results with an expert, and also analyzing inter-operator variability. As a second endpoint, we compare this method by analyzing semen parameters before and after cryopreservation. Methods: Four professional trainees studied and practiced semen analysis according to the World Health Organization guidelines for one month, under supervision of an expert in the field. Next, microscopic results (sperm concentration, motility, vitality, and morphology) obtained by each team member were compared with the findings obtained by the expert.. Finally, analyzes of inter-operators were evaluated for the same parameters. Results: The findings obtained by the operators and the expert did not differ significantly. Furthermore, in the inter-operator analysis, the morphology parameter differed significantly in the fresh semen sample, which was not observed in the post-thaw sample. Conclusion: Our results indicated that the laboratory staff training for semen analysis was effective, ensuring the assessment of individual performance and uniformity among operators in sperm count parameters, producing consistent results.