Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1395
10
1
gregorian
2017
1
1
11
1
online
1
fulltext
fa
Optimization of Extracellular Cellulase Production by Trichoderma harzianum
تحقيقي
Original Paper
<div><strong>ABSTRACT</strong><br>
<strong> Background and Objectives:</strong> Cellulose is a major component of plant biomass, which can be converted into biofuels and valuable chemicals. The key step in utilization of this organic material is its hydrolysis into soluble sugars. This study evaluated cellulase production by Trichoderma harzianum under different pH values, temperatures and incubation periods with the aim to increase enzyme production and decrease its costs.<br>
<strong>Methods:</strong> The amount of protein production and the hydrolytic activity of cellulase enzymes including exoglucanase, endoglucanase and β-glucosidase produced by T. harzianum were evaluated using various substrates such as avicel, carboxymethyl cellulose, cellobiose, Whatman grade 1 filter paper under different pH values (4, 4.8, 5.5 and 6.5), temperatures (25, 28 and 34 °C) and incubation times (48, 72, 96 and 120 h).<br>
<strong>Results:</strong> The optimum condition for cellulase production by T. harzianum is 120 hours of incubation at 25 °C and pH of 6.5.<br>
<strong>Conclusion:</strong> T. harzianum can be used for the production of all three classes of cellulase. This fungus is suitable for the efficient production of cellulolytic enzymes and reducing the cost of consumables.<br>
<strong>Keywords:</strong> Cellulose, Trichoderma harzianum, Hydrolytic enzymes, Optimization.</div>
Cellulose, Trichoderma harzianum, Hydrolytic enzymes, Optimization.
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32
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-445&slc_lang=fa&sid=1
Tabassom
Naseri Poor
naseripour1391@gmail.com
100319475328460012493
100319475328460012493
Yes
Department of plant protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran
saeed
Nasrollahnejad
100319475328460012494
100319475328460012494
No
Department of plant protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran
Samira
Shahbazi
100319475328460012495
100319475328460012495
No
Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Alborz, Iran
Kamran
Rahnama
100319475328460012496
100319475328460012496
No
Department of plant protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran