Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1395
6
1
gregorian
2016
9
1
10
5
online
1
fulltext
en
Impact of Ionizing Radiation on the Expression of CDC25A Phosphatase (in vivo)
تحقيقي
Original Paper
<div>
<p><strong>Background and Objective: </strong>The cell division cycle 25 (CDC25)is a familyof highly conserved dual-specificity phosphatases that activate cyclin-dependent kinase complexes. These complexes are the main cell cycle regulators. Mammalian cells ,exposure to DNA damaging radiations such as ionizing radiation and ultraviolet light, prevent cell cycle progression by activation of checkpoint pathways and lead to cell death.</p>
<p><strong> Methods:</strong> In this study, mice were exposed to different doses of ionizing radiation. Their total cellular protein was extracted from the bone marrow. After determining and matching the protein concentrations, CDC25A phosphatase levels were measured by western blotting.</p>
<p><strong> Results:</strong> The results showed that exposure to different doses of ionizing radiation in vivo significantly increased the expression of CDC25A compared to control group (P <0.05).</p>
<p><strong> Conclusion:</strong> Exposure to ionizing radiation increases the expression of CDC25A phosphatase, which increases the possibility of tumorigenesis in that area by increasing bone marrow cell proliferation.</p>
<p><strong> Keywords:</strong> Cell Cycle, CDC25A, Ionizing Radiation, Cyclin-Dependent Kinase.</p>
</div>
Cell Cycle, CDC25A, Ionizing Radiation, Cyclin-Dependent Kinase
22
26
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-419&slc_lang=en&sid=1
Seyed Mostafa
Mir
100319475328460011503
100319475328460011503
No
Department of Clinical Biochemistry
Esmaeil
Samadian
100319475328460011504
100319475328460011504
No
Laboratory Sciences Research Center
Sahar
Alijanpour
100319475328460011505
100319475328460011505
No
Department of Medical Genetics
Alireza
Khoshbin Khoshnazar
100319475328460011506
100319475328460011506
No
Department of Clinical Biochemistry
Hamid
Haghighatfard
100319475328460011507
100319475328460011507
No
Department of Medical Biotechnology
Seyed Hossein
Sadeghi
Hsadeqi86@gmail.com
100319475328460011508
100319475328460011508
Yes
Department of Medical Genetics